LOCUS       pF_AA124        4728 bp DNA     circular SYN 30-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             450..452
                     /label=Start
     CDS             456..2306
                     /codon_start=1
                     /label=p300_v1.2
                     /translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
                     VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
                     EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
                     ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
                     FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
                     SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
                     PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
                     IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
                     GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
                     RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
                     MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
     CDS             2307..2546
                     /label=Linker_v3.1
     CDS             2547..2567
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40NLS_v1.6
                     /translation="PKKKRKV"
     misc_feature    2568..2580
                     /label=GG-3R
     CDS             2568..2573
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(3040..3628)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3806..4615)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagccacca tggccatttt caagcctgaa gagctgagac
      481 aagccctgat gcccaccctg gaggccctgt atcggcagga ccctgagagc ctgcccttca
      541 gacagcctgt ggatcctcag ctgctgggaa tccctgatta cttcgacatc gttaagtctc
      601 ctatggatct gtctaccatc aaacgcaagc tggacaccgg ccagtaccag gagccttggc
      661 agtacgtgga cgacatctgg ctgatgttca acaacgcctg gctgtacaac agaaagacca
      721 gcagagtgta caagtactgc agcaagctga gcgaggtctt tgagcaagag atcgaccctg
      781 tgatgcagtc tctgggctat tgttgcggca gaaagctgga attcagccct caaacactgt
      841 gctgctacgg caagcagctg tgcaccatcc cacgggatgc tacatactac agctaccaga
      901 atagatacca cttctgcgag aagtgcttta acgagattca gggcgagtcc gtgtccctgg
      961 gcgacgaccc cagccagccc cagaccacca tcaacaaaga gcagttcagc aagcgcaaga
     1021 acgacaccct ggatcccgag ctttttgtgg aatgtaccga gtgcggcagg aagatgcacc
     1081 agatctgcgt gctgcaccac gagatcatct ggcccgccgg cttcgtgtgc gacggctgtc
     1141 tgaagaagag cgccagaacc cggaaagaga acaagttcag tgctaagaga ctgcctagca
     1201 ccagactggg aacattcctg gaaaataggg tgaacgactt cctgcggcgg caaaaccacc
     1261 cggagtctgg agaagtcacc gtgcgggtgg tgcacgccag cgacaagacc gtggaagtga
     1321 aacctggcat gaaggccaga ttcgtggaca gcggagagat ggccgagagc ttcccctaca
     1381 gaacaaaggc cctgttcgcc ttcgaggaaa tcgacggtgt tgatctgtgt tttttcggca
     1441 tgcatgtgca ggagtacggc tctgactgcc ctccaccaaa ccagagaaga gtctatatca
     1501 gctacctgga ctctgtgcat ttcttccggc ccaaatgcct gagaacagcc gtgtaccacg
     1561 agatcctcat cggctacctc gagtacgtga agaaactggg ctacaccaca ggccacatct
     1621 gggcctgtcc tccatctgaa ggcgacgact acatcttcca ctgccaccct cctgaccaga
     1681 aaatccctaa gcccaagaga ctgcaggagt ggtacaaaaa aatgctggac aaggctgtga
     1741 gcgaaagaat cgtgcacgac tacaaggaca tctttaagca ggccactgaa gatagactga
     1801 cctccgccaa ggagctgcct tacttcgagg gcgatttctg gcctaatgtg ctggaggaat
     1861 ctatcaagga actggagcag gaggaggaag agcggaagcg ggaagaaaat acctccaacg
     1921 agtctacaga cgtgaccaag ggcgacagca agaacgctaa gaagaaaaac aacaagaaaa
     1981 ccagcaaaaa caagagcagc ctgtccagag gcaacaaaaa aaagcctgga atgcctaacg
     2041 tcagcaacga cctgagccag aagctgtacg ccacaatgga aaagcacaag gaagtgttct
     2101 ttgtgatcag actgattgcc ggacctgccg ccaatagcct gcctcccatc gtcgatccag
     2161 accccctgat cccctgtgat ctgatggacg gcagagatgc tttcctgacc ctggccagag
     2221 ataagcacct ggaattcagc agcctgagaa gagcccagtg gagcaccatg tgcatgctgg
     2281 tggaactgca cacacagagc caggacggag ggccgagctc tggcgcaccc ccaccaagtg
     2341 gagggtctcc tgccgggtcc ccaacatcta ctgaagaagg caccagcgaa tccgcaacgc
     2401 ccgagtcagg ccctggtacc tccacagaac catctgaagg tagtgcgcct ggttccccag
     2461 ctggaagccc tacttccacc gaagaaggca cgtcaaccga accaagtgaa ggatctgccc
     2521 ctgggaccag cactgaacca tctgagccaa aaaagaagag aaaggtaggc agcagtcttc
     2581 atcggatccc gggcccgtcg actgcagagg cctgcatgca agcttggtgt aatcatggtc
     2641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaagcccaa tctgaataat
     3781 gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca
     3841 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     3901 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     3961 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     4021 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     4081 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     4141 ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa
     4201 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     4261 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt tttccgggga
     4321 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     4381 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     4441 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaagcgat
     4501 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     4561 catccatgtt ggaatttaat cgcggcctcg acgtttcccg ttgaatatgg ctcataacac
     4621 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     4681 cttgtgcaat gtaacatcag agattttgag acacgggcca gagctgca
//
