LOCUS       pUC57-Kan        2639 bp DNA     circular SYN 12-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pUC57-Kan
FEATURES             Location/Qualifiers
     source          1..2639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             452..454
                     /label=Start
     CDS             458..478
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40NLS_v1.19
                     /translation="PKKKRKV"
     misc_feature    479..491
                     /label=GG-3R
     CDS             479..484
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(951..1539)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1717..2526)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggaccgccac catggtgcct aagaagaaga gaaaggtggg
      481 cagcagtctt catcggatcc cgggcccgtc gactgcagag gcctgcatgc aagcttggtg
      541 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      601 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      661 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaagccca
     1681 atctgaataa tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
     1741 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     1801 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     1861 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     1921 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     1981 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     2041 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     2101 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     2161 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     2221 ttttccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     2281 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     2341 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     2401 atacaagcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     2461 atataaatca gcatccatgt tggaatttaa tcgcggcctc gacgtttccc gttgaatatg
     2521 gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga
     2581 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgca
//
