LOCUS       pUC57-Kan        2693 bp DNA     circular SYN 16-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pUC57-Kan
FEATURES             Location/Qualifiers
     source          1..2693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             454..456
                     /label=Start
     CDS             457..528
                     /codon_start=1
                     /label=u(S)ORF_v1.1
                     /translation="VSKGEELFTGVVPILVELDGSR*"
     CDS             526..528
                     /codon_start=1
                     /label=Stop
                     /translation=""
     CDS             530..532
                     /label=Start
     misc_feature    533..545
                     /label=GG-3R
     CDS             533..538
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1005..1593)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1771..2580)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagaattcc aacatggtga gcaagggcga ggagctgttc
      481 accggggtgg tgcccatcct ggtcgagctg gacggctcta gataataaca tgggcagcag
      541 tcttcatcgg atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggtgtaatca
      601 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      661 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      721 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      781 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      841 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      901 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      961 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1021 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1081 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1141 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1201 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1261 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1321 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1381 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1441 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1501 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1561 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1621 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1681 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaag cccaatctga
     1741 ataatgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     1801 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     1861 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     1921 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     1981 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     2041 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     2101 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     2161 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     2221 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     2281 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     2341 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     2401 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     2461 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     2521 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat
     2581 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     2641 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gca
//
