LOCUS       pUC57-Kan        2750 bp DNA     circular SYN 16-OCT-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pUC57-Kan
FEATURES             Location/Qualifiers
     source          1..2750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             454..456
                     /label=Start
     CDS             457..528
                     /codon_start=1
                     /label=u(S)ORF_v1.1
                     /translation="VSKGEELFTGVVPILVELDGSR*"
     CDS             526..528
                     /label=Stop
     CDS             530..532
                     /label=Start
     CDS             536..589
                     /codon_start=1
                     /label=BPNLS_v1.1
                     /translation="KRTADGSEFESPKKKRKV"
     misc_feature    590..602
                     /label=GG-3R
     CDS             590..595
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1062..1650)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1828..2637)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=aph(3')-Ia
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagaattcc aacatggtga gcaagggcga ggagctgttc
      481 accggggtgg tgcccatcct ggtcgagctg gacggctcta gataataaca tggccaaacg
      541 gacagccgac ggaagcgagt tcgagtcacc aaagaagaag cggaaagtcg gcagcagtct
      601 tcatcggatc ccgggcccgt cgactgcaga ggcctgcatg caagcttggt gtaatcatgg
      661 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
      721 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
      781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
      841 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
      901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      961 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1021 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1081 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1141 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1201 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1261 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1321 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1381 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1441 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1501 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1561 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1621 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1681 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1741 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaagccc aatctgaata
     1801 atgttacaac caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg
     1861 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     1921 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     1981 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     2041 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     2101 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     2161 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     2221 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     2281 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg tttttccggg
     2341 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     2401 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc
     2461 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaagcg
     2521 atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     2581 agcatccatg ttggaattta atcgcggcct cgacgtttcc cgttgaatat ggctcataac
     2641 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt
     2701 atcttgtgca atgtaacatc agagattttg agacacgggc cagagctgca
//
