LOCUS       pUC57-Kan        3208 bp DNA     circular SYN 25-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pUC57-Kan
FEATURES             Location/Qualifiers
     source          1..3208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             450..452
                     /label=Start
     CDS             453..1046
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR_v1.2
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    1047..1060
                     /label=GG-5R
     CDS             1047..1052
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1520..2108)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2286..3095)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagccacca tgaccgagta caagcccacg gtgcgcctcg
      481 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
      541 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
      601 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
      661 ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
      721 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
      781 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggagtct
      841 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
      901 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
      961 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     1021 ggtgcatgac ccgcaagccc ggtgccggaa gcacgtcttc atcggatccc gggcccgtcg
     1081 actgcagagg cctgcatgca agcttggtgt aatcatggtc atagctgttt cctgtgtgaa
     1141 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1201 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1261 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1321 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1381 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1441 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1501 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1561 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1621 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1681 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1741 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1801 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1861 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     1921 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     1981 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2041 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2101 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2161 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2221 attaaaaatg aagttttaaa tcaagcccaa tctgaataat gttacaacca attaaccaat
     2281 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     2341 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     2401 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     2461 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     2521 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     2581 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     2641 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     2701 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     2761 ggatattctt ctaatacctg gaatgctgtt tttccgggga tcgcagtggt gagtaaccat
     2821 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     2881 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     2941 agaaacaact ctggcgcatc gggcttccca tacaagcgat agattgtcgc acctgattgc
     3001 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     3061 cgcggcctcg acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     3121 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     3181 agattttgag acacgggcca gagctgca
//
