LOCUS       pUC57-Kan        3328 bp DNA     circular SYN 10-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pUC57-Kan
FEATURES             Location/Qualifiers
     source          1..3328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             450..452
                     /label=Start
     CDS             453..1166
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=VexGFP_v1.1
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLFIQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    1167..1180
                     /label=GG-5R
     CDS             1167..1172
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1640..2228)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2406..3215)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagccacca tggtgagcaa gggcgaggag ctgttcaccg
      481 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      541 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      601 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcagctac ggcgtgcagt
      661 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      721 aaggctacgt ccaggagcgc accatcagct tcaaggacga cggcaactac aagacccgcg
      781 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      841 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      901 tctatatcac ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca
      961 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1021 acggccccgt gctgctgccc gacaaccact acctgttcat ccagtccgcc ctgagcaaag
     1081 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1141 ctcacggcat ggacgagctg tacaagggaa gcacgtcttc atcggatccc gggcccgtcg
     1201 actgcagagg cctgcatgca agcttggtgt aatcatggtc atagctgttt cctgtgtgaa
     1261 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1321 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1381 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1441 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1501 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1561 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1621 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1681 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1741 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     1801 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     1861 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     1921 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     1981 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2041 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2101 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2161 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2221 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2281 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2341 attaaaaatg aagttttaaa tcaagcccaa tctgaataat gttacaacca attaaccaat
     2401 tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta
     2461 tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag
     2521 ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata
     2581 caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg
     2641 acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca
     2701 ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt
     2761 gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga
     2821 atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca
     2881 ggatattctt ctaatacctg gaatgctgtt tttccgggga tcgcagtggt gagtaaccat
     2941 gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc
     3001 cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc
     3061 agaaacaact ctggcgcatc gggcttccca tacaagcgat agattgtcgc acctgattgc
     3121 ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat
     3181 cgcggcctcg acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     3241 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     3301 agattttgag acacgggcca gagctgca
//
