LOCUS       pUC57-Kan        3322 bp DNA     circular SYN 02-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   GenScript
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     Sequence Label: pUC57-Kan
FEATURES             Location/Qualifiers
     source          1..3322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-2F
     CDS             450..452
                     /label=Start
     CDS             453..1160
                     /codon_start=1
                     /label=TagRFP_v1.1
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     misc_feature    1161..1174
                     /label=GG-5R
     CDS             1161..1166
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1634..2222)
                     /direction=LEFT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2400..3209)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgta ggagccacca tggtgtctaa gggcgaagag ctgattaagg
      481 agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac ttcaagtgca
      541 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
      601 agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg tacggcagca
      661 gaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc ttccctgagg
      721 gcttcacatg ggagagagtc accacatacg aggacggggg cgtgctgacc gctacccagg
      781 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttcc
      841 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac accgagatgc
      901 tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag ctcgtgggcg
      961 ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc gctaagaacc
     1021 tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag gaggccgaca
     1081 aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac ctccctagca
     1141 aactggggca caaacttaat ggaagcacgt cttcatcgga tcccgggccc gtcgactgca
     1201 gaggcctgca tgcaagcttg gtgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1261 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1321 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1381 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1441 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2041 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2341 aatgaagttt taaatcaagc ccaatctgaa taatgttaca accaattaac caattctgat
     2401 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     2461 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     2521 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     2581 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     2641 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
     2701 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     2761 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     2821 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     2881 tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa ccatgcatca
     2941 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     3001 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     3061 aactctggcg catcgggctt cccatacaag cgatagattg tcgcacctga ttgcccgaca
     3121 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     3181 ctcgacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     3241 gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt
     3301 tgagacacgg gccagagctg ca
//
