LOCUS       pF_AA199        3205 bp DNA     circular SYN 10-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-4F
     CDS             438..443
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     CDS             444..986
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65_v1.1
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             996..1043
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=NpNLS_v1.1
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    1044..1057
                     /label=GG-5R
     CDS             1044..1049
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1517..2105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2283..3092)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacggg tccccttcag ggcagatcag caaccaggcc ctggctctgg
      481 cccctagctc cgctccagtg ctggcccaga ctatggtgcc ctctagtgct atggtgcctc
      541 tggcccagcc acctgctcca gcccctgtgc tgaccccagg accaccccag tcactgagcg
      601 ctccagtgcc caagtctaca caggccggcg aggggactct gagtgaagct ctgctgcacc
      661 tgcagttcga cgctgatgag gacctgggag ctctgctggg gaacagcacc gatcccggag
      721 tgttcacaga tctggcctcc gtggacaact ctgagtttca gcagctgctg aatcagggcg
      781 tgtccatgtc tcatagtaca gccgaaccaa tgctgatgga gtaccccgaa gccattaccc
      841 ggctggtgac cggcagccag cggccccccg accccgctcc aactcccctg ggaaccagcg
      901 gcctgcctaa tgggctgtcc ggagatgagg acttctcaag catcgctgat atggacttta
      961 gtgccctgct gtcacagatt tcctctggca ccagcaaaag gccggcggcc acgaaaaagg
     1021 ccggccaggc aaaaaagaaa aagggaagca cgtcttcatc ggatcccggg cccgtcgact
     1081 gcagaggcct gcatgcaagc ttggtgtaat catggtcata gctgtttcct gtgtgaaatt
     1141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1261 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1321 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1921 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2101 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2221 aaaaatgaag ttttaaatca agcccaatct gaataatgtt acaaccaatt aaccaattct
     2281 gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
     2341 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
     2401 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
     2461 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
     2521 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
     2581 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
     2641 tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
     2701 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
     2761 tattcttcta atacctggaa tgctgttttt ccggggatcg cagtggtgag taaccatgca
     2821 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
     2881 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
     2941 aacaactctg gcgcatcggg cttcccatac aagcgataga ttgtcgcacc tgattgcccg
     3001 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
     3061 ggcctcgacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     3121 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     3181 ttttgagaca cgggccagag ctgca
//
