LOCUS       pF_AA200        3601 bp DNA     circular SYN 10-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-4F
     CDS             438..443
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     CDS             444..986
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65_v1.2
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             1011..1382
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1_v1.1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             1392..1439
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=NpNLS_v1.1
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    1440..1453
                     /label=GG-5R
     CDS             1440..1445
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1913..2501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2679..3488)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacggg tccccttcag ggcagatcag caaccaggcc ctggctctgg
      481 cccctagctc cgctccagtg ctggcccaga ctatggtgcc ctctagtgct atggtgcctc
      541 tggcccagcc acctgctcca gcccctgtgc tgaccccagg accaccccag tcactgagcg
      601 ctccagtgcc caagtctaca caggccggcg aggggactct gagtgaagct ctgctgcacc
      661 tgcagttcga cgctgatgag gacctgggag ctctgctggg gaacagcacc gatcccggag
      721 tgttcacaga tctggcctcc gtggacaact ctgagtttca gcagctgctg aatcagggcg
      781 tgtccatgtc tcatagtaca gccgaaccaa tgctgatgga gtaccccgaa gccattaccc
      841 ggctggtgac cggcagccag cggccccccg accccgctcc aactcccctg ggaaccagcg
      901 gcctgcctaa tgggctgtcc ggagatgaag atttctcaag catcgctgat atggacttta
      961 gtgccctgct gtcacagatt tcctctagtg ggcagggagg aggtggaagc ggcttcagcg
     1021 tggacaccag tgccctgctg gacctgttca gcccctcggt gaccgtgccc gacatgagcc
     1081 tgcctgacct tgacagcagc ctggccagta tccaagagct cctgtctccc caggagcccc
     1141 ccaggcctcc cgaggcagag aacagcagcc cggattcagg gaagcagctg gtgcactaca
     1201 cagcgcagcc gctgttcctg ctggaccccg gctccgtgga caccgggagc aacgacctgc
     1261 cggtgctgtt tgagctggga gagggctcct acttctccga aggggacggc ttcgccgagg
     1321 accccaccat ctccctgctg acaggctcgg agcctcccaa agccaaggac cccactgtct
     1381 ccggcaccag caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     1441 gaagcacgtc ttcatcggat cccgggcccg tcgactgcag aggcctgcat gcaagcttgg
     1501 tgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1561 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1621 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1681 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     1741 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1801 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1861 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1921 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1981 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2041 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2101 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2161 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2221 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2281 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2341 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2401 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2461 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2521 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2581 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaagcc
     2641 caatctgaat aatgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
     2701 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     2761 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     2821 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     2881 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     2941 agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     3001 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     3061 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     3121 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     3181 gtttttccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     3241 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     3301 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     3361 ccatacaagc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     3421 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgacgtttc ccgttgaata
     3481 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgat
     3541 gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacggg ccagagctgc
     3601 a
//
