LOCUS       pF_AA302        2704 bp DNA     circular SYN 09-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2704
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-4F
     CDS             438..443
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     CDS             444..491
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=NpNLS_v1.2
                     /translation="KRPAATKKAGQAKKKK"
     CDS             492..494
                     /codon_start=1
                     /label=Stop
                     /translation=""
     polyA_signal    495..542
                     /label=polyA_v1
                     /note="Platt et al Cell. 2014 Sep 24. pii: 
                     S0092-8674(14)01163-5. doi: 10.1016/j.cell.2014.09.014."
     misc_feature    543..556
                     /label=GG-5R
     CDS             543..548
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1016..1604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1782..2591)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacggg tccaagcggc ccgcagcaac caagaaggca ggacaggcca
      481 agaagaagaa gtaaaataaa agatctttat tttcattaga tctgtgtgtt ggttttttgt
      541 gtggaagcac gtcttcatcg gatcccgggc ccgtcgactg cagaggcctg catgcaagct
      601 tggtgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      661 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      721 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      781 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      841 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      901 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      961 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1021 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1081 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1141 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1201 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1261 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1321 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1381 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1441 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1501 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1561 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1621 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1681 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1741 gcccaatctg aataatgtta caaccaatta accaattctg attagaaaaa ctcatcgagc
     1801 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     1861 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     1921 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     1981 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     2041 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     2101 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     2161 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     2221 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     2281 gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     2341 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     2401 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     2461 ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     2521 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttga
     2581 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     2641 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc
     2701 tgca
//
