LOCUS       pF_AA428        2812 bp DNA     circular SYN 07-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-4F
     CDS             438..443
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     CDS             444..491
                     /codon_start=1
                     /product="bipartite nuclear localization signal 
                     fromnucleoplasmin"
                     /label=NpNLS_v1.1
                     /translation="KRPAATKKAGQAKKKK"
     CDS             498..578
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA_v1.1
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             579..605
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-mycNLS_v1.2
                     /translation="PAAKRVKLD"
     CDS             624..650
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-mycNLS_v1.3
                     /translation="PAAKRVKLD"
     misc_feature    651..664
                     /label=GG-5R
     CDS             651..656
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     rep_origin      complement(1124..1712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1890..2699)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacggg tccaaaaggc cggcggccac gaaaaaggcc ggccaggcaa
      481 aaaagaaaaa gggtagctac ccatacgatg ttccagatta cgcttatccc tacgacgtgc
      541 ctgattatgc atacccatat gatgtccccg actatgcccc ggcagctaag agggtgaaac
      601 tggatggcgg tagcggcgga ggccccgccg ccaagcgcgt gaagctggac ggaagcacgt
      661 cttcatcgga tcccgggccc gtcgactgca gaggcctgca tgcaagcttg gtgtaatcat
      721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1561 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaagc ccaatctgaa
     1861 taatgttaca accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac
     1921 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1981 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     2041 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     2101 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     2161 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     2221 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     2281 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     2341 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgtttttccg
     2401 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     2461 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     2521 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaag
     2581 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     2641 tcagcatcca tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata
     2701 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     2761 ttatcttgtg caatgtaaca tcagagattt tgagacacgg gccagagctg ca
//
