LOCUS       pF_AA163        2633 bp DNA     circular SYN 09-MAY-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-5F
     CDS             438..443
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     CDS             444..446
                     /label=Stop
     misc_feature    466..477
                     /label=GG-6R
     rep_origin      complement(945..1533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1711..2520)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgga agctaatagg actcagttac gatgcattcc agtcttcgga
      481 accaaatcgg atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggtgtaatca
      541 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      601 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      661 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      721 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
      781 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      841 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      901 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      961 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1021 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1081 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1141 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1201 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1261 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1321 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1381 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1441 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1501 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1561 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1621 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaag cccaatctga
     1681 ataatgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     1741 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     1801 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     1861 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     1921 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     1981 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     2041 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     2101 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     2161 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     2221 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     2281 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     2341 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     2401 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     2461 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat
     2521 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     2581 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gca
//
