LOCUS       pF_AA303        2993 bp DNA     circular SYN 09-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    432..443
                     /label=GG-5F
     CDS             438..443
                     /codon_start=1
                     /label=GS
                     /translation="GS"
     promoter        444..692
                     /label=U6_v1
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    689..699
                     /label=BsmBI_v0
     misc_feature    701..706
                     /label=6T
     misc_feature    707..717
                     /label=BsmBI_v6
     misc_feature    714..810
                     /label=trRNA_v1 [Nme2]
     misc_feature    811..825
                     /label=terminator_v1
     misc_feature    826..837
                     /label=GG-9R
     rep_origin      complement(1305..1893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2071..2880)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagagaatt cgagctcggt acctcgcgaa
      421 tacatctaga tgaagacgga agcgagggcc tatttcccat gattccttca tatttgcata
      481 tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
      541 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      601 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      661 ctttatatat cttgtggaaa ggacgaaaca ccggagacgc ttttttcgtc tcagttgtag
      721 ctcccgaaac gttgctacaa taaggccgtc tgaaaagatg tgccgcaacg ctctgcccct
      781 taaagcttct gctttaaggg gcatcgttta tttttttgtt taaaccgtcc agtcttcgga
      841 accaaatcgg atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggtgtaatca
      901 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      961 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     1021 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     1081 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1141 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1201 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1261 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1321 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1381 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1441 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1501 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1561 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1621 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1681 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1741 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1801 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1861 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1921 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1981 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaag cccaatctga
     2041 ataatgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa
     2101 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     2161 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     2221 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     2281 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg
     2341 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     2401 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     2461 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     2521 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgtttttcc
     2581 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     2641 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     2701 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     2761 gcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     2821 atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt tcccgttgaa tatggctcat
     2881 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     2941 tttatcttgt gcaatgtaac atcagagatt ttgagacacg ggccagagct gca
//
