LOCUS       pF_AA269        4165 bp DNA     circular SYN 12-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 21, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    11..22
                     /label=GG-6F
     misc_feature    23..611
                     /label=WPRE_v1
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_difference 561
                     /label=Silent
     misc_difference 655
                     /label=Silent
     promoter        710..958
                     /label=U6_v1
     misc_feature    955..965
                     /label=BsmBI_v0
     misc_feature    967..972
                     /label=6T
     misc_feature    973..983
                     /label=BsmBI_v1
     misc_feature    980..1065
                     /label=trRNA_v4 [Sp]
     LTR             1118..1298
                     /label=5' LTR (truncated)
     polyA_signal    1330..1554
                     /label=bGHpolyA_v1
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1577..1588
                     /label=GG-9R
     rep_origin      complement(2056..2644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2822..3631)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 acatctagat gaagacggat tcaatcaacc tctggattac aaaatttgtg aaagattgac
       61 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
      121 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
      181 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
      241 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
      301 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
      361 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
      421 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
      481 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
      541 tctgcggcct cttccgcgtc ctcgccttcg ccctcagacg agtcggatct ccctttgggc
      601 cgcctccccg cgtcggctag cacactggaa gggctaattc actcccaacg aagataagat
      661 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt
      721 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      781 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      841 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      901 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      961 agacgctttt ttcgtctcag tttaagagct atgctggaaa cagcatagca agtttaaata
     1021 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt taagcttggc
     1081 gtaactagat cttgagacac tgctttttgc ttgtactggg tctctctggt tagaccagat
     1141 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1201 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1261 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     1321 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     1381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     1441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     1501 gggaggattg ggaagataat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     1561 aggcggaaag aaccagcgtc cagtcttcgg aaccaaatcg gatcccgggc ccgtcgactg
     1621 cagaggcctg catgcaagct tggtgtaatc atggtcatag ctgtttcctg tgtgaaattg
     1681 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     1741 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     1801 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     1861 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     1921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     1981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     2041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     2101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     2161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     2221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     2281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     2341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     2401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     2461 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     2521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     2581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     2641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     2701 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     2761 aaaatgaagt tttaaatcaa gcccaatctg aataatgtta caaccaatta accaattctg
     2821 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     2881 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     2941 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     3001 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     3061 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     3121 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     3181 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     3241 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     3301 attcttctaa tacctggaat gctgtttttc cggggatcgc agtggtgagt aaccatgcat
     3361 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     3421 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     3481 acaactctgg cgcatcgggc ttcccataca agcgatagat tgtcgcacct gattgcccga
     3541 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     3601 gcctcgacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     3661 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
     3721 tttgagacac gggccagagc tgcatcgcgc gtttcggtga tgacggtgaa aacctctgac
     3781 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     3841 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     3901 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     3961 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     4021 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     4081 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagag
     4141 aattcgagct cggtacctcg cgaat
//
