LOCUS       pRDA_575        4668 bp DNA     circular SYN 28-MAR-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   jdoench
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4668
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          537..539
                     /label=chr 5
     misc_feature    540..1023
                     /label=Piggbac 3' cassette (w insulator)
     repeat_region   546..608
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1031..1042
                     /label=GG-1R
     promoter        1048..1272
                     /label=EFS/SCP3 promoter
     promoter        1048..1191
                     /label=EFS partial
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    1192..1272
                     /label=SCP3
     misc_feature    1197..1204
                     /label=TATA
     misc_feature    1226..1231
                     /label=INR
     misc_structure  1228
                     /label=TSS
     misc_feature    1245..1260
                     /label=MTE/DPE
     5'UTR           1274..1320
                     /label=alphaGlobin-MO
     regulatory      1321..1330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1327..2001
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     misc_feature    2011..2022
                     /label=GG_6F
     polyA_signal    2047..2168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2169..2481
                     /label=Piggbac 5' cassette (w insulator)
     repeat_region   2440..2474
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(2941..3529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3700..4491,4492..4560))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4561..4665)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
       61 taaaaatagg cgtatcacga ggccctttcg tcttgcgcgt ttcggtgatg acggtgaaaa
      121 cctctgacac atgcagctcc cggaaacggt cacagcttgt ctgtaagcgg atgccgggag
      181 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      241 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
      301 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
      361 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      421 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      481 ggccagtgaa ttcgagctcg gtacctcgcg aatgcatcta gatatcgacg gccagtcccg
      541 attaacccta gaaagataat catattgtga cgtacgttaa agataatcat gtgtaaaatt
      601 gacgcatgtg ttttatcggt ctgtatatcg aggtttattt attaatttga atagatatta
      661 agttttatta tatttacact tacatactaa taataaattc aacaaacaat ttatttatgt
      721 ttatttattt attaaaaaaa acaaaaactc aaaatttctt ctataaagta acaaaacttt
      781 tatgagggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag
      841 ggggcagcag cgagccgccc ccggctccgc tccggtccgg cgctcccccc gcatccccga
      901 gccggcagcg tgcggggaca gcccgcgcac ggggaaggtg gcacgggatc gctttcctct
      961 gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaggcct
     1021 ccaggtacct acacgcgtct tccatgcggg cagagcgcac atcgcccaca gtccccgaga
     1081 agttgggggg aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact
     1141 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaggtctata
     1201 taagcagagc tcgtttagtg aaccgtcagt ccgcctggag acctcgagcc gagtggtcgt
     1261 gcctccatag aaagaataaa ctagtattct tctggtcccc acagactcag agagaacccc
     1321 gccaccatgg atagcactga gaacgtcatc aagcccttca tgcgcttcaa ggtgcacatg
     1381 gagggctccg tgaacggcca cgagttcgag atcgagggcg tgggcgaggg caagccctac
     1441 gagggcaccc agaccgccaa gctgcaagtg accaagggcg gccccctgcc cttcgcctgg
     1501 gacatcctgt ccccccagtt cttctacggc tccaaggcgt acatcaagca ccccgccgac
     1561 atccccgact acctcaagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     1621 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcaccctc
     1681 atctaccacg tgaagttcat cggcgtgaac ttcccctccg acggccccgt aatgcagaaa
     1741 aagactctgg gctgggagcc ctccactgag cgcaactacc cccgcgacgg cgtgctgaag
     1801 ggcgagaacc acatggcgct gaagctgaag ggcggcggcc actacctgtg tgagttcaag
     1861 tccatctaca tggccaagaa gcccgtgaag ctgcccggct accactacgt ggactacaag
     1921 ctcgacatca cctcccacaa cgaggactac accgtggtgg agcagtacga gcgcgccgag
     1981 gcccgccacc acctgttcca gtaaatgcat gaagacgcat tcgaattcca tgcctttgta
     2041 tcagttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     2101 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     2161 gtatcttaat ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg
     2221 aaataacaat ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg
     2281 cgtcattttg actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca
     2341 agcacgcctc acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat
     2401 tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga
     2461 ctatctttct agggttaatc tatcggatcc cgggcccgtc gactgcagag gcctgcatgc
     2521 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2581 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2641 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2701 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2761 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3361 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3601 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3661 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3721 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3781 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3841 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3901 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3961 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4021 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4081 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4141 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4201 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4261 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4321 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4381 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4441 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4501 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4561 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4621 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcac
//
