LOCUS       pRDA_791        1877 bp DNA     circular SYN 07-JUN-2022
DEFINITION  McGee_LO_13_pRDA_791     coverage: 68x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    50..61
                     /label=GG_0R
     misc_feature    74..85
                     /label=GG_6F
     promoter        114..218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(219..287,288..1079)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1250..1837
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtc agaacgtctt
       61 ctgactcgag gacgaagacg cattctttag gtggcacttt tcggggaaat gtgcgcggaa
      121 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      181 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      241 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      301 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      361 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
      421 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
      481 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
      541 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
      601 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
      661 cttttttgca caacatgggg gaccatgtaa ctcgccttga tcgttgggaa ccggagctga
      721 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
      781 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
      841 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
      901 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
      961 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1021 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1081 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1141 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1201 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1261 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1321 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1381 agatacaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1441 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1501 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1561 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1621 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1681 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1741 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1801 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1861 acggttcctg gcctttt
//
