LOCUS       pRDB_051        3516 bp DNA     circular SYN 31-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     328..344
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(381..422)
                     /label=LacZalpha
                     /note="LacZalpha"
     protein_bind    384..417
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     misc_feature    423..865
                     /label=GPP destination cassette flanked by BbsI sites
     misc_feature    423..434
                     /label=GG_0R
     misc_feature    854..865
                     /label=GG_6F
     misc_feature    complement(866..1237)
                     /label=LacZalpha
                     /note="LacZalpha"
     terminator      886..1050
                     /label=SV40 polyA
                     /note="SV40 polyA"
     misc_feature    897..916
                     /label=EBVrev primer
                     /note="EBVrev primer"
     misc_feature    1198..1237
                     /label=attB40
                     /note="attB40"
     protein_bind    1201..1234
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     complement(1295..1311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1319..1335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1343..1349,1350..1367,1368..1373))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1388..1409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1697..2285)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(2456..3247,3248..3316))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3317..3421)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
       61 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
      121 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
      181 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      241 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      301 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta
      361 cctcgcgaat gcatctagat cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac
      421 ttcagaacgt cttcaccagt aatttacgtg ggaaaggtgc ttgtgtccca acaggctagg
      481 atataatgct gaaaccgtcc cccaagcgtt cagggtgggg tttgctacaa cttccgagtc
      541 caatgtgtcc gtgttcatga tatatatgct caagggcgag aattggacct agctttcgtg
      601 ttagtacgta gcatggtgac acaagcacag tagatcctgc ccgccgatcg tatatattaa
      661 gttaattctt atggaatata ataacatgtg gatggccagt ggtcggttgt tacacgccta
      721 ccgcgatgct gaatgacccg gactagagtg gcgaaattta tggcgtgtga cccgttatgc
      781 tccatttcgg tcagtgggtc attgctagta gtcgattgca ttgccattct ccgagtgatt
      841 tatggacccc agtgaagacg cattcggatc cgaattcaga catgataaga tacattgatg
      901 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
      961 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     1021 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     1081 acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt gagagccttc
     1141 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatggta
     1201 cgcgcccggg gagcccaagg gcacgccctg gcacccgatc ggatcccggg cccgtcgact
     1261 gcagaggcct gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     1321 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     1381 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1441 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1501 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1561 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1621 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1681 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1741 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1801 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1861 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1921 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1981 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2041 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2101 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     2161 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2221 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2281 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2341 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2401 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2461 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2521 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2581 ctgcaatgat accgcgagat ccacgctcac cggctccaga tttatcagca ataaaccagc
     2641 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2701 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2761 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2821 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2881 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2941 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3001 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3061 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3121 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3181 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3241 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3301 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3361 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3421 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3481 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
