LOCUS       pRDB_053        9652 bp DNA     circular SYN 31-JAN-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 20, 2023 from SnapGene 7.1.1
            https://www.snapgene.com
COMMENT     LOCUS       GU931385                9842 bp    DNA     circular SYN 
            20-JUN-2012
            DEFINITION  Overexpression vector pWALIUM10-moe, complete sequence.
            ACCESSION   GU931385
            VERSION     GU931385.2  GI:392347481
            KEYWORDS    .
            SOURCE      Overexpression vector pWALIUM10-moe
              ORGANISM  Overexpression vector pWALIUM10-moe
                        other sequences; artificial sequences; vectors.
            REFERENCE   1  (bases 1 to 9842)
              AUTHORS   Ni,J.-Q., Liu,L.-P., Perkins,L.A. and Perrimon,N.
              TITLE     Vectors for transgenic flies
              JOURNAL   Unpublished
            REFERENCE   2  (bases 1 to 9842)
              AUTHORS   Ni,J.-Q., Liu,L.-P., Perkins,L.A. and Perrimon,N.
              TITLE     Direct Submission
              JOURNAL   Submitted (22-FEB-2010) Genetics, Harvard Medical 
            School, 77 Avenue
                        Louis Pasteur, Boston, MA 02115, USA
            REFERENCE   3  (bases 1 to 9842)
              AUTHORS   Hu,Y. and Perkins,L.A.
              TITLE     Direct Submission
              JOURNAL   Submitted (20-JUN-2012) Genetics, Harvard Medical 
            School, 77 Avenue
                        Louis Pasteur, Boston, MA 02115, USA
              REMARK    Sequence update by submitter
            COMMENT     On Jun 20, 2012 this sequence version replaced 
            gi:291501163.
FEATURES             Location/Qualifiers
     source          1..9652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          5344..5348
                     /label=Overexpression vector pWALIUM10-moe
     source          8575..8581
                     /label=Overexpression vector pWALIUM10-moe
     misc_feature    126
                     /label=T->G Fix mut in attB site
     misc_feature    229..659
                     /label=gypsy insulator
                     /note="gypsy insulator"
     misc_feature    656..659
                     /label=reverse
                     /note="reverse"
     misc_feature    660..1102
                     /label=GPP destination cassette flanked by BbsI sites
     misc_feature    660..671
                     /label=GG_0R
     misc_feature    1091..1102
                     /label=GG_6F
     intron          1149..1295
                     /label=ftz intron
                     /note="ftz intron"
     misc_feature    1319..2018
                     /label=SV40 polyA region
                     /note="SV40 polyA region"
     misc_feature    complement(1611..1630)
                     /label=sv40R
                     /note="sv40R"
     misc_feature    1612
                     /label=T->A BbsI mut
     misc_feature    2025..2455
                     /label=gypsy insulator
                     /note="gypsy insulator"
     misc_feature    3677..4336
                     /label=AmpR
                     /note="AmpR"
     misc_feature    5349..9455
                     /label=white
                     /note="white"
     misc_feature    5654
                     /label=T->A BsmBI mut
     misc_feature    5929
                     /label=C->T BbsI mut
     CDS             complement(join(6111..6725,6796..6927,7131..7446,7508..8162,
                     8237..8510,8912..8983))
                     /codon_start=1
                     /label=white
                     /note="selectable marker"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    6231
                     /label=G->A BsmBI mut
     misc_feature    6603
                     /label=C->T BbsI mut
     misc_feature    7606
                     /label=T->C BbsI mut
     intron          8163..8236
                     /label=white intron
                     /note="white intron"
     misc_feature    8575..8581
                     /label=white
                     /note="white"
     misc_feature    9232
                     /label=T->A BsmBI mut
     misc_recomb     join(9502..9652,1..217)
                     /label=attB docking site
                     /note="attB docking site"
ORIGIN
        1 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
       61 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
      121 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
      181 tcgacaagcc gaattgatcc actagaaggc ctaattcggt acactagttg gccacgtaat
      241 aagtgtgcgt tgaatttatt cgcaaaaaca ttgcatattt tcggcaaagt aaaattttgt
      301 tgcatacctt atcaaaaaat aagtgctgca tactttttag agaaaccaaa taatttttta
      361 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
      421 actttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacccg
      481 tttttaataa aatacattgc ataccctctt ttaataaaga atattgcata cgttgacgaa
      541 acaaattttc gttgcatacc caataaaaga ttattatatt gcataccttt tcttgccata
      601 ccatttagcc gatcaattgt gctcggcaac agtatatttg tggtgtgcca accaacaacc
      661 agaacgtctt caccagtaat ttacgtggga aaggtgcttg tgtcccaaca ggctaggata
      721 taatgctgaa accgtccccc aagcgttcag ggtggggttt gctacaactt ccgagtccaa
      781 tgtgtccgtg ttcatgatat atatgctcaa gggcgagaat tggacctagc tttcgtgtta
      841 gtacgtagca tggtgacaca agcacagtag atcctgcccg ccgatcgtat atattaagtt
      901 aattcttatg gaatataata acatgtggat ggccagtggt cggttgttac acgcctaccg
      961 cgatgctgaa tgacccggac tagagtggcg aaatttatgg cgtgtgaccc gttatgctcc
     1021 atttcggtca gtgggtcatt gctagtagtc gattgcattg ccattctccg agtgatttat
     1081 ggaccccagt gaagacgcat tctctagagc aaactagttc tgatctgcta gacaattgtt
     1141 ggcatcaggt aggcatcaca cacgattaac aacccctaaa aatacacttt gaaaatattg
     1201 aaaatatgtt tttgtataca tttttgatat tttcaaataa tacgcagtta taaaactcat
     1261 tagctaaccc attttttctt tgcttatgct tacagattgc aaagaactag agccgcggga
     1321 tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga
     1381 tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc
     1441 taattgtttg tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga
     1501 atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg
     1561 ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta gtagacccca
     1621 aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc
     1681 ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta
     1741 tggaaaaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     1801 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1861 atgaatggaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1921 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1981 tccaaactca tcaatgtatc ttatcatgtc tggttccaga gctctggcca cgtaataagt
     2041 gtgcgttgaa tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca
     2101 taccttatca aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc
     2161 atacccgttt ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt
     2221 tgacgaaaca aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt
     2281 taataaaata cattgcatac cctcttttaa taaagaatat tgcatacgtt gacgaaacaa
     2341 attttcgttg catacccaat aaaagattat tatattgcat accttttctt gccataccat
     2401 ttagccgatc aattgtgctc ggcaacagta tatttgtggt gtgccaacca acaacgagct
     2461 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     2521 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     2581 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     2641 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     2701 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2761 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2821 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2881 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     2941 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3001 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3061 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3121 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3181 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3241 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3301 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     3361 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3421 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3481 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3541 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3601 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3661 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3721 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3781 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3841 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3901 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     3961 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4021 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4081 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4141 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4201 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4261 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4321 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4381 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4441 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4501 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4561 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4621 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     4681 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4741 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4801 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4861 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4921 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4981 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     5041 tttcggccta ttggttaaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     5101 aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc
     5161 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     5221 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     5281 agcgcgcgta atacgactca ctatagggcg aattgggtac aagcttcacg acctaggcgc
     5341 gccctagttc cagtgaaatc caagcatttt ctaaattaaa tgtattctta ttattatagt
     5401 tgttattttt gatatatata aacaacacta ttatgcccac catttttttg agatgcatct
     5461 acacaaggaa caaacactgg atgtcacttt cagttcaaat tgtaacgcta atcactccga
     5521 acaggtcaca aaaaattacc ttaaaaagtc ataatattaa attagaataa atatagctgt
     5581 gagggaaata tatacaaata tattggagca aataaattgt acatacaaat atttattact
     5641 aatttctatt gagtcgaaat gaaccactcg gaaccatttg agcgaaccga atcgcgcgga
     5701 actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg gagagcgggt
     5761 gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt atgaagaggg
     5821 cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg tagcgataaa
     5881 cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaaa caaatccaaa
     5941 gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca tatcttgttt
     6001 ttattggcac aaatataatt acattagctt tttgaggggg caataaacag taaacacgat
     6061 ggtaataatg gtaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
     6121 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
     6181 gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt ttccaggatg
     6241 accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc
     6301 acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac
     6361 gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaagcc gccaaagagc
     6421 aggaatggta tgataaccgg cggacccaca gacagcgcca tcgaggtcga ggagctggcg
     6481 caggatatta gatatccgaa ggacgttgac acattggcca ccagagtgac cagcgccagg
     6541 cagttgaaga agtgcagcac tccggcccgc agtccgatca tcggataggc aatcgccgtg
     6601 aaaaccagtg gcactgtgag aaaaagcggc aattcggcaa tcgttttgcc cagaaagtat
     6661 gtgtcacagc gataaagtcg acttcgggcc tccctcataa aaactggcag ctctgaggtg
     6721 aacacctaaa tcgaatcgat tcattagaaa gttagtaaat tattgaaatg caaatgtatt
     6781 ctaaacatga cttacattta tcgtggcaaa gacgttttga aaggtcatgt tggtcaggaa
     6841 gaggaagatg gctccgttga tattcatcac acccacttgc gtgagttgtt ggcccaaaaa
     6901 gatgaggcca atcaagatgg caaccatctg caaattaaaa tgttactcgc atctcattaa
     6961 tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa ctataaacta tacatctcat
     7021 tgaaaaaaac taagaagggt gtggaatcag gcaattctat ctaaaatcta gcgaatttgt
     7081 ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac cgttgtctga
     7141 ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga tcgccacagg
     7201 accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc cggctgctcc
     7261 agtggcttct ccagattttt ggtggccaac aactgctcca tatcccgggc tactttgcta
     7321 atggcaaaat tgtcgcatat cttggcgatc cgatcacggg actcgatctc ccgtccgggc
     7381 acaacggcca acacctgtac gtaaaagtcc gccggattgt agttggtagg acactgggca
     7441 cccacgctgg ataggagttg agatgttatg taatactaga tacccttaat aaacacatcg
     7501 aactcactag gaaaagaagt cgacggcttc gctgggagtg cccaagaaag ctaccctgcc
     7561 ctcggccatc agaaggatct tgtcaaagag ctcaaacagc tcggaggacg gctgatgaat
     7621 ggtcaggatg acggtcttgc ccttctgcga cagcttcttc agcacctgga cgacgctgtg
     7681 ggcggtaaag gagtccagtc cggaggtggg ctcatcgcag atcagaagcg gcggatcggt
     7741 tagagcctcg gaggcgaatg ccagacgctt cctttctccg ccggacagac ctttcaccct
     7801 gccgggcaca ccgatgatcg tgtgctgaca tttgctgagc gaaagctcct ggatcacctg
     7861 atccacgcgg gccactcgct gccgataggt cagatgtcgt ggcatccgca ccatggcttg
     7921 gaaaatcagg tgttccctgg ccgttaggga gccgataaag aggtcatcct gctggacata
     7981 ggcgcacctg gcctgcatct ccttggcgtc cacaggttgg ccattgagca gtcgcatccc
     8041 ggatggcgat acttggatgc cctgcggcga tcgaaaggca agggcattca gcagggtcgt
     8101 ctttccggca ccggaactgc ccatcacggc caaaagttcg cccggatagg ccacgccgca
     8161 aactgagttt caaattggta attggaccct ttattaagat ttcacacaga tcagccgact
     8221 gcgaatagaa actcaccgtt cttgagcaaa tgtttcctgg gcgccggtat gtgtcgctcg
     8281 ttgcagaata gtccgcgtgt ccggttgacc agctgccgcc atccggagcc cggctgattg
     8341 accgccccaa agatgtccat attgtgccag gcataggtga ggttctcggc tagttggccg
     8401 ctccctgaac cggagtcctc cggcggactg ggtggccgga gcgtgccgta gtttttggcc
     8461 tgcccgaagc cctggttaat gcagctctgc gaagccgctc cgctgtcacc ctgcaatgat
     8521 aggggatctc aaatatcaac tacaagcgtt atgctcatct aaccccgaac aaaaagtacc
     8581 ccgaagtatc ctacgaagta ggtttatact tttatttatt ttttgtgcat ctaggatcag
     8641 cttaaaatat ctggttgtta tattttttgt aaaaaagaat atagtcgaaa atgaatgcct
     8701 ttagatgtct tgatcatgat atgatctcaa aaattgtctt atatagcgag aacagctacc
     8761 agaataatct gtttcgtgtc actatttgtt tgtgcaattg cggtttggga tttttgtggg
     8821 tcgcagttct cacgccgcag acaatttgat gttgcaatcg cagttcctat agatcaagtg
     8881 aacttaagat gtatgcacat gtactactca cattgttcag atgctcggca gatgggtgtt
     8941 tgctgcctcc gcgaattaat agctcctgat cctcttggcc cattgccggg atttttcaca
     9001 ctttcccctg cttacccacc caaaaccaat caccacccca atcactcaaa aaacaaacaa
     9061 aaataagaag cgagaggagt tttggcacag cactttgtgt ttaattgatg gcgtaaaccg
     9121 cttggagctt cgtcacgaaa ccgctgacaa aatgcaactg aaggcggaca ttgacgctac
     9181 gtaacgctac aaacggtggc gaaagagata gcggacgcag cggcgaaaga gtcggcgata
     9241 tttctgtgga cagagaagga ggcaaacagc gctgactttg agtggaatgt cattttgagt
     9301 gagaggtaat cgaaagaacc tggtacatca aatacccttg gatcgaagta aatttaaaac
     9361 tgatcagata agttcaatga tatccagtgc agtaaaaaaa aaatgttttt tttatctact
     9421 ttccgcaaaa atgggtttta ttaacttaca tacatactag gcgcgccaga tcgcaagaag
     9481 cttgatatca tcgatctcga ggctgcatcc aacgcgttgg gagctctccg gatcaattcg
     9541 gcttcaggta ccgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg ggtgccaggg
     9601 cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca tc
//
