LOCUS       pMV_AA090              13337 bp ds-DNA     circular SYN 15-DEC-2023
DEFINITION  Modular plasmid designed using Fragmid, a plasmid design tool of
            the Genetic Perturbation Platform (GPP), Broad Institute
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13337)
  AUTHORS   Genetic Perturbation Platform (GPP), Broad Institute
COMMENT     Fragmid source plasmids: pRDA_722, pF_AA179, pF_AA065, pF_AA012,
            pF_AA008, pF_AA009, pF_AA006
FEATURES             Location/Qualifiers
     enhancer        238..617
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        619..817
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     LTR             835..1015
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    1062..1187
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Psi"
     misc_feature    1680..1913
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     misc_feature    2440..2557
                     /note="CPPT"
                     /label="CPPT"
     misc_feature    2615..2635
                     /label="Filler"
     promoter        2640..3818
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
                     /label="EF1a_v1"
     CDS             3829..3831
                     /codon_start=1
                     /transl_table=1
                     /label="Start"
     CDS             3835..3888
                     /codon_start=1
                     /transl_table=1
                     /translation="KRTADGSEFESPKKKRKV"
                     /label="BPNLS_v1.1"
     CDS             3895..7812
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /transl_table=1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYR
                     NAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRS
                     FDKFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLR
                     EHFENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGL
                     NEVLNLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCK
                     YKTLLRNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERR
                     ISELTGKITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALD
                     QPLPTTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEP
                     SLSFYNKARNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGI
                     MPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTP
                     ILLSNNFIEPLEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDF
                     LSKYTKTTSIDLSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLY
                     LFQIYNKDFAKGHHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMA
                     HRLGEKMLNKKLKDQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHE
                     IIKDRRFTSDKFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLI
                     YITVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLS
                     QVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDY
                     PAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIK
                     NHESRKHFLEGFDFLHYDVKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQF
                     DAKGTPFIAGKRIVPVIENHRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLE
                     NDDSHAIDTMVALIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDAD
                     ANGAYHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN"
                     /label="Cas12a_v1.1 [EnAs]"
     misc_difference 4411..4413
                     /label="E174R"
     misc_difference 5515..5517
                     /label="S542R"
     misc_difference 5533..5535
                     /label="K548R"
     CDS             7813..7818
                     /codon_start=1
                     /transl_table=1
                     /translation="GS"
                     /label="GS"
     CDS             7819..7866
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /transl_table=1
                     /translation="KRPAATKKAGQAKKKK"
                     /label="NpNLS_v1.1"
     CDS             7873..7953
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /transl_table=1
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
                     /label="3xHA_v1.1"
     CDS             7954..7959
                     /codon_start=1
                     /transl_table=1
                     /translation="GS"
                     /label="GS"
     CDS             7960..8013
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A_v1.1"
     CDS             8023..8415
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /note="confers resistance to blasticidin"
                     /product="blasticidin S deaminase"
                     /transl_table=1
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPG
                     IKAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BlastR_v1.1"
     CDS             8416..8418
                     /codon_start=1
                     /transl_table=1
                     /label="Stop"
     misc_feature    8429..9017
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
                     /label="WPRE"
     misc_feature    8967
                     /label="Silent"
     LTR             9089..9322
                     /note="self-inactivating 3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (Delta-U3)"
     misc_feature    9118
                     /label="Silent"
     polyA_signal    9354..9578
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     misc_feature    9541
                     /label="Silent"
     rep_origin      9624..10052
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        10066..10395
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      10246..10381
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        10443..10490
                     /note="synthetic bacterial promoter"
                     /label="EM7 promoter"
     CDS             10509..10883
                     /codon_start=1
                     /note="BleomycinR"
                     /transl_table=1
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD*"
                     /label="BleomycinR"
     polyA_signal    11013..11134
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      complement(11585..12173)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(12344..13204)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /product="beta-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /label="AmpR"
     promoter        complement(13205..13309)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT ACGCGTTGAC
      241 ATTGATTATT GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT
      301 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG
      361 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT
      421 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG
      481 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC
      541 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG
      601 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT
      661 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC
      721 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG
      781 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGCGC GTTTTGCCTG TACTGGGTCT
      841 CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT
      901 AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC
      961 TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC
     1021 GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC
     1081 GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA
     1141 TTTTGACTAG CGGAGGCTAG AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG
     1201 GGAGAATTAG ATCGCGATGG GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA
     1261 AATTAAAACA TATAGTATGG GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC
     1321 TGTTAGAAAC ATCAGAAGGC TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA
     1381 CAGGATCAGA AGAACTTAGA TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC
     1441 AAAGGATAGA GATAAAAGAC ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA
     1501 AAAGTAAGAC CACCGCACAG CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG
     1561 AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA
     1621 GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA
     1681 GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG
     1741 ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG
     1801 CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG
     1861 CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT
     1921 TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT
     1981 AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT
     2041 AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG
     2101 AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA
     2161 ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA
     2221 AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA
     2281 TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA
     2341 GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG TGAACGGATC GGCACTGCGT
     2401 GCGCCAATTC TGCAGACAAA TGGCAGTATT CATCCACAAT TTTAAAAGAA AAGGGGGGAT
     2461 TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA GCAACAGACA TACAAACTAA
     2521 AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG GTTTATTACA GGGACAGCAG
     2581 AGATCCAGTT TGGTTAATTA AGGTACCGGG CAGAGGTCTC GCTTTTTTCG AGACAACACG
     2641 GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG
     2701 GAGGGGTCGG CAATTGATCC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG
     2761 ATGTCGTGTA CTGGCTCCGC CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG
     2821 TAGTCGCCGT GAACGTTCTT TTTCGCAACG GGTTTGCCGC CAGAACACAG GTAAGTGCCG
     2881 TGTGTGGTTC CCGCGGGCCT GGCCTCTTTA CGGGTTATGG CCCTTGCGTG CCTTGAATTA
     2941 CTTCCACCTG GCTGCAGTAC GTGATTCTTG ATCCCGAGCT TCGGGTTGGA AGTGGGTGGG
     3001 AGAGTTCGAG GCCTTGCGCT TAAGGAGCCC CTTCGCCTCG TGCTTGAGTT GAGGCCTGGC
     3061 CTGGGCGCTG GGGCCGCCGC GTGCGAATCT GGTGGCACCT TCGCGCCTGT CTCGCTGCTT
     3121 TCGATAAGTC TCTAGCCATT TAAAATTTTT GATGACCTGC TGCGACGCTT TTTTTCTGGC
     3181 AAGATAGTCT TGTAAATGCG GGCCAAGATC TGCACACTGG TATTTCGGTT TTTGGGGCCG
     3241 CGGGCGGCGA CGGGGCCCGT GCGTCCCAGC GCACATGTTC GGCGAGGCGG GGCCTGCGAG
     3301 CGCGGCCACC GAGAATCGGA CGGGGGTAGT CTCAAGCTGG CCGGCCTGCT CTGGTGCCTG
     3361 GCCTCGCGCC GCCGTGTATC GCCCCGCCCT GGGCGGCAAG GCTGGCCCGG TCGGCACCAG
     3421 TTGCGTGAGC GGAAAGATGG CCGCTTCCCG GCCCTGCTGC AGGGAGCTCA AAATGGAGGA
     3481 CGCGGCGCTC GGGAGAGCGG GCGGGTGAGT CACCCACACA AAGGAAAAGG GCCTTTCCGT
     3541 CCTCAGCCGT CGCTTCATGT GACTCCACGG AGTACCGGGC GCCGTCCAGG CACCTCGATT
     3601 AGTTCTCGAG CTTTTGGAGT ACGTCGTCTT TAGGTTGGGG GGAGGGGTTT TATGCGATGG
     3661 AGTTTCCCCA CACTGAGTGG GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT
     3721 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
     3781 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAAG GAGCCACCAT GGCCAAACGG
     3841 ACAGCCGACG GAAGCGAGTT CGAGTCACCA AAGAAGAAGC GGAAAGTCGG CAGCACACAG
     3901 TTCGAGGGCT TTACCAACCT GTATCAGGTG AGCAAGACAC TGCGGTTTGA GCTGATCCCA
     3961 CAGGGCAAGA CCCTGAAGCA CATCCAGGAG CAGGGCTTCA TCGAGGAGGA CAAGGCCCGC
     4021 AATGATCACT ACAAGGAGCT GAAGCCCATC ATCGATCGGA TCTACAAGAC CTATGCCGAC
     4081 CAGTGCCTGC AGCTGGTGCA GCTGGATTGG GAGAACCTGA GCGCCGCCAT CGACTCCTAT
     4141 AGAAAGGAGA AAACCGAGGA GACAAGGAAC GCCCTGATCG AGGAGCAGGC CACATATCGC
     4201 AATGCCATCC ACGACTACTT CATCGGCCGG ACAGACAACC TGACCGATGC CATCAATAAG
     4261 AGACACGCCG AGATCTACAA GGGCCTGTTC AAGGCCGAGC TGTTTAATGG CAAGGTGCTG
     4321 AAGCAGCTGG GCACCGTGAC CACAACCGAG CACGAGAACG CCCTGCTGCG GAGCTTCGAC
     4381 AAGTTTACAA CCTACTTCTC CGGCTTTTAT CGGAACAGGA AGAACGTGTT CAGCGCCGAG
     4441 GATATCAGCA CAGCCATCCC ACACCGCATC GTGCAGGACA ACTTCCCCAA GTTTAAGGAG
     4501 AATTGTCACA TCTTCACACG CCTGATCACC GCCGTGCCCA GCCTGCGGGA GCACTTTGAG
     4561 AACGTGAAGA AGGCCATCGG CATCTTCGTG AGCACCTCCA TCGAGGAGGT GTTTTCCTTC
     4621 CCTTTTTATA ACCAGCTGCT GACACAGACC CAGATCGACC TGTATAACCA GCTGCTGGGA
     4681 GGAATCTCTC GGGAGGCAGG CACCGAGAAG ATCAAGGGCC TGAACGAGGT GCTGAATCTG
     4741 GCCATCCAGA AGAATGATGA GACAGCCCAC ATCATCGCCT CCCTGCCACA CAGATTCATC
     4801 CCCCTGTTTA AGCAGATCCT GTCCGATAGG AACACCCTGT CTTTCATCCT GGAGGAGTTT
     4861 AAGAGCGACG AGGAAGTGAT CCAGTCCTTC TGCAAGTACA AGACACTGCT GAGAAACGAG
     4921 AACGTGCTGG AGACAGCCGA GGCCCTGTTT AACGAGCTGA ACAGCATCGA CCTGACACAC
     4981 ATCTTCATCA GCCACAAGAA GCTGGAGACA ATCAGCAGCG CCCTGTGCGA CCACTGGGAT
     5041 ACACTGAGGA ATGCCCTGTA TGAGCGGAGA ATCTCCGAGC TGACAGGCAA GATCACCAAG
     5101 TCTGCCAAGG AGAAGGTGCA GCGCAGCCTG AAGCACGAGG ATATCAACCT GCAGGAGATC
     5161 ATCTCTGCCG CAGGCAAGGA GCTGAGCGAG GCCTTCAAGC AGAAAACCAG CGAGATCCTG
     5221 TCCCACGCAC ACGCCGCCCT GGATCAGCCA CTGCCTACAA CCCTGAAGAA GCAGGAGGAG
     5281 AAGGAGATCC TGAAGTCTCA GCTGGACAGC CTGCTGGGCC TGTACCACCT GCTGGACTGG
     5341 TTTGCCGTGG ATGAGTCCAA CGAGGTGGAC CCCGAGTTCT CTGCCCGGCT GACCGGCATC
     5401 AAGCTGGAGA TGGAGCCTTC TCTGAGCTTC TACAACAAGG CCAGAAATTA TGCCACCAAG
     5461 AAGCCCTACT CCGTGGAGAA GTTCAAGCTG AACTTTCAGA TGCCTACACT GGCCCGCGGC
     5521 TGGGACGTGA ATCGGGAGAA GAACAATGGC GCCATCCTGT TTGTGAAGAA CGGCCTGTAC
     5581 TATCTGGGCA TCATGCCAAA GCAGAAGGGC AGGTATAAGG CCCTGAGCTT CGAGCCCACA
     5641 GAGAAAACCA GCGAGGGCTT TGATAAGATG TACTATGACT ACTTCCCTGA TGCCGCCAAG
     5701 ATGATCCCAA AGTGCAGCAC CCAGCTGAAG GCCGTGACAG CCCACTTTCA GACCCACACA
     5761 ACCCCCATCC TGCTGTCCAA CAATTTCATC GAGCCTCTGG AGATCACAAA GGAGATCTAC
     5821 GACCTGAACA ATCCTGAGAA GGAGCCAAAG AAGTTTCAGA CAGCCTACGC CAAGAAAACC
     5881 GGCGACCAGA AGGGCTACAG AGAGGCCCTG TGCAAGTGGA TCGACTTCAC AAGGGATTTT
     5941 CTGTCCAAGT ATACCAAGAC AACCTCTATC GATCTGTCTA GCCTGCGGCC ATCCTCTCAG
     6001 TATAAGGACC TGGGCGAGTA CTATGCCGAG CTGAATCCCC TGCTGTACCA CATCAGCTTC
     6061 CAGAGAATCG CCGAGAAGGA GATCATGGAT GCCGTGGAGA CAGGCAAGCT GTACCTGTTC
     6121 CAGATCTATA ACAAGGACTT TGCCAAGGGC CACCACGGCA AGCCTAATCT GCACACACTG
     6181 TATTGGACCG GCCTGTTTTC TCCAGAGAAC CTGGCCAAGA CAAGCATCAA GCTGAATGGC
     6241 CAGGCCGAGC TGTTCTACCG CCCTAAGTCC AGGATGAAGA GGATGGCACA CCGGCTGGGA
     6301 GAGAAGATGC TGAACAAGAA GCTGAAGGAT CAGAAAACCC CAATCCCCGA CACCCTGTAC
     6361 CAGGAGCTGT ACGACTATGT GAATCACAGA CTGTCCCACG ACCTGTCTGA TGAGGCCAGG
     6421 GCCCTGCTGC CCAACGTGAT CACCAAGGAG GTGTCTCACG AGATCATCAA GGATAGGCGC
     6481 TTTACCAGCG ACAAGTTCTT TTTCCACGTG CCTATCACAC TGAACTATCA GGCCGCCAAT
     6541 TCCCCATCTA AGTTCAACCA GAGGGTGAAT GCCTACCTGA AGGAGCACCC CGAGACACCT
     6601 ATCATCGGCA TCGATCGGGG CGAGAGAAAC CTGATCTATA TCACAGTGAT CGACTCCACC
     6661 GGCAAGATCC TGGAGCAGCG GAGCCTGAAC ACCATCCAGC AGTTTGATTA CCAGAAGAAG
     6721 CTGGACAACA GGGAGAAGGA GAGGGTGGCA GCAAGGCAGG CCTGGTCTGT GGTGGGCACA
     6781 ATCAAGGATC TGAAGCAGGG CTATCTGAGC CAGGTCATCC ACGAGATCGT GGACCTGATG
     6841 ATCCACTACC AGGCCGTGGT GGTGCTGGAG AACCTGAATT TCGGCTTTAA GAGCAAGAGG
     6901 ACCGGCATCG CCGAGAAGGC CGTGTACCAG CAGTTCGAGA AGATGCTGAT CGATAAGCTG
     6961 AATTGCCTGG TGCTGAAGGA CTATCCAGCA GAGAAAGTGG GAGGCGTGCT GAACCCATAC
     7021 CAGCTGACAG ACCAGTTCAC CTCCTTTGCC AAGATGGGCA CCCAGTCTGG CTTCCTGTTT
     7081 TACGTGCCTG CCCCATATAC ATCTAAGATC GATCCCCTGA CCGGCTTCGT GGACCCCTTC
     7141 GTGTGGAAAA CCATCAAGAA TCACGAGAGC CGCAAGCACT TCCTGGAGGG CTTCGACTTT
     7201 CTGCACTACG ACGTGAAAAC CGGCGACTTC ATCCTGCACT TTAAGATGAA CAGAAATCTG
     7261 TCCTTCCAGA GGGGCCTGCC CGGCTTTATG CCTGCATGGG ATATCGTGTT CGAGAAGAAC
     7321 GAGACACAGT TTGACGCCAA GGGCACCCCT TTCATCGCCG GCAAGAGAAT CGTGCCAGTG
     7381 ATCGAGAATC ACAGATTCAC CGGCAGATAC CGGGACCTGT ATCCTGCCAA CGAGCTGATC
     7441 GCCCTGCTGG AGGAGAAGGG CATCGTGTTC AGGGATGGCT CCAACATCCT GCCAAAGCTG
     7501 CTGGAGAATG ACGATTCTCA CGCCATCGAC ACCATGGTGG CCCTGATCCG CAGCGTGCTG
     7561 CAGATGCGGA ACTCCAATGC CGCCACAGGC GAGGACTATA TCAACAGCCC CGTGCGCGAT
     7621 CTGAATGGCG TGTGCTTCGA CTCCCGGTTT CAGAACCCAG AGTGGCCCAT GGACGCCGAT
     7681 GCCAATGGCG CCTACCACAT CGCCCTGAAG GGCCAGCTGC TGCTGAATCA CCTGAAGGAG
     7741 AGCAAGGATC TGAAGCTGCA GAACGGCATC TCCAATCAGG ACTGGCTGGC CTACATCCAG
     7801 GAGCTGCGCA ACGGGTCCAA AAGGCCGGCG GCCACGAAAA AGGCCGGCCA GGCAAAAAAG
     7861 AAAAAGGGAT CCTACCCATA CGATGTTCCA GATTACGCTT ATCCCTACGA CGTGCCTGAT
     7921 TATGCATACC CATATGATGT CCCCGACTAT GCCGGAAGCG AGGGCAGAGG AAGCCTTCTA
     7981 ACATGCGGTG ACGTGGAGGA GAATCCCGGC CCTTCCGGGA TGGCCAAGCC TTTGTCTCAA
     8041 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAG
     8101 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT
     8161 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT
     8221 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG
     8281 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA
     8341 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT
     8401 TATGTGTGGG AGGGCTAATA AATTCGAAAA TCAACCTCTG GATTACAAAA TTTGTGAAAG
     8461 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT
     8521 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC
     8581 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG
     8641 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT
     8701 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT
     8761 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG
     8821 GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC
     8881 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT
     8941 GCCGGCTCTG CGGCCTCTTC CGCGTCCTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT
     9001 TTGGGCCGCC TCCCCGCGTC GACTTTAAGA CCAATGACTT ACAAGGCAGC TGTAGATCTT
     9061 AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA TTCACTCCCA ACGAAGATAA
     9121 GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC
     9181 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA
     9241 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG
     9301 TCAGTGTGGA AAATCTCTAG CAGGGCCCGT TTAAACCCGC TGATCAGCCT CGACTGTGCC
     9361 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG
     9421 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG
     9481 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA
     9541 TAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGGCT TCTGAGGCGG AAAGAACCAG
     9601 CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT
     9661 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC
     9721 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG
     9781 GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA
     9841 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT
     9901 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT
     9961 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA
    10021 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG
    10081 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG
    10141 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG
    10201 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT
    10261 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG
    10321 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC
    10381 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC
    10441 GTGTTGACAA TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA
    10501 ACTAAACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG
    10561 GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT
    10621 TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG
    10681 TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT
    10741 GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG
    10801 GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT
    10861 TCGTGGCCGA GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT
    10921 ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG
    10981 GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT
    11041 ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA
    11101 GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCGACCTCTA
    11161 GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA
    11221 CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG
    11281 TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT
    11341 CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC
    11401 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG
    11461 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA
    11521 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG
    11581 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA
    11641 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG
    11701 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG
    11761 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC
    11821 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG
    11881 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA
    11941 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT
    12001 GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG
    12061 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG
    12121 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC
    12181 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT
    12241 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT
    12301 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA
    12361 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG
    12421 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC
    12481 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG
    12541 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC
    12601 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA
    12661 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC
    12721 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC
    12781 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC
    12841 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT
    12901 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA
    12961 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT
    13021 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
    13081 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
    13141 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
    13201 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG
    13261 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC
    13321 GAAAAGTGCC ACCTGAC
//
