LOCUS       pYHY2                   7446 bp ds-DNA     circular     01-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    110..1213
                     /label="mobA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1464..2198
                     /label="ermG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2467..2630
                     /label="BfP1E6 promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2631..2663
                     /label="RBS8"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    2664..2804
                     /label="BT0294 SP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             2664..2804
                     /label="Translation 2664-2804"
                     /translation="MRNLKWLYACSLAIAFGVLSFVTVSCHDDDDEPKQEPGEVIETPAPV"
     misc_feature    2805..3314
                     /label="Nanoluc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             2805..3314
                     /label="Translation 2805-3314"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA"
     CDS             3315..3335
                     /label="Translation 3315-3335"
                     /translation="GSSGSSG"
     misc_feature    3315..3335
                     /label="flexible linker"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    3336..3401
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3336..3404
                     /label="Translation 3336-3404"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK*"
     terminator      3428..3499
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(3606..4466)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    4674..5262
                     /label="pBR322 ori"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     oriT            5422..5531
                     /label="oriT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    6164..6853
                     /label="RepA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 CAATCTTCAA AGTCGGGTGG TTGTCAAGAT GATTTTTTTG GTTTGGTGTC GTCTTTTTTT
       61 AAGCTGCCGC ATAACGGTTT GGCGCCGATT GTCACAAAAA TGTTAACTTA TGGGAGCAAC
      121 AAGTATTCAT GTACAAGCAG TGAAGCCGGG GAGCGAGATT CACAACTTTA GGGAAAAAGA
      181 GTTGGACTAT GTTCGTCCCG AACTTAGTCA TTTGAATGAA AGCTGGGTTG GAGATAGCAT
      241 TTCCCATCGG CTGGAGAGTG CAAAACAAAG ATATCTCGAT ACGGTTGGGC AGAAGATGCA
      301 GGCTAAAGCC GCACCCATAC GAGAGGGAGT AATAGTAATC AAACAAGAAA CCACTATGCA
      361 GGAACTCCAG CAGTTTGCCA CGGTCTGCAA AGAACGTTTC GGTATCGAAG CGTTTCAAAT
      421 CCATATACAC AAGGACGAAG GATACATGAA CGCAAAGCAG TGGACACCTA ACCTTCATGC
      481 CCATGTAGTT TTCGATTGGA CGCAGCCGAA TGGGAAAAGT GTGCGTTTAT CGCGTGATGA
      541 CATGGCAGAA CTCCAAACCA TAGCATCTGA AACACTGGGC ATGGAACGTG GCGTTTCTTC
      601 TGACCGCAAA CACTTATCGG CTATGCAGTA CAAGACCGAA TGTGCGAAAG AACAGCTTCA
      661 GGAACTATCC AACGATATAT CCAGTGCATT AGACAAATAC AAGGACGCAC AAAACCAGCT
      721 TCTTCAGCTT CAGAAGGAAC TACGTTCCAT TGAAACAAAG AAGAACGTCC AAAAACTCAT
      781 TTCTAAGGCT TCAGAGAAGT TTTACGGCTT GATTGGAAAA ACAGTCAACG ATAGGGAAAA
      841 GGATGCCTTA AAAGCCAAAG TAAAGACTTT AGAGGGGGAA AATGAACAAC TATCCGATAG
      901 ACTGGGAAAG GCTATACTTG AAAAAGAGCG AAACAGCACA AAGGCATTCA AAGCCGAGAA
      961 TGACAAGGAG TATTATAGAC AACAAATGGA CAACGCAAGG ACAACCAGTA ACCTATTAAG
     1021 GACGGAGAAC CAAAAACTAA AAGCCGAAAC CAAGGAGTTG AAAAAAGAAC TTGGTAAGAT
     1081 GAAAGACTTG TTCAATTCCG AACAACTGGA GGCTTTAAGG CATCATTTCC CAAACATATC
     1141 AAAAGCTATG GAAGAGGGGA AAGACCTATT CAAGCAAATC ACCAGAAGCA GAGGTTTCGG
     1201 CATGGGTATG TAACCCCCCC CCGCCCCCAA AGGGGGAGCG ACCAAACGGC AGCCTCTCTC
     1261 AATGGAGTGT TACatccCGT AAAGAGGGGC TTTGCAGGGG GTGGACTCAG AAAGATGAGA
     1321 ATAGATGACT ATTGTAGTTG AAACACATAG AAAGTTGCTG ATATACAGAC CGATACGCAT
     1381 ATCGGGATGA ACCATGAGTA CGTTCTTTTC TCAAAAAACA TAAATATTCG AAAAGAGATG
     1441 CAATAAATTA AGGAGAGGTT ATAATGAACA AAGTAAATAT AAAAGATAGT CAAAATTTTA
     1501 TTACTTCAAA ATATCACATA GAAAAAATAA TGAATTGCAT AAGTTTAGAT GAAAAAGATA
     1561 ACATCTTTGA AATAGGTGCA GGGAAAGGTC ATTTTACTGC TGGATTGGTA AAGAGATGTA
     1621 ATTTTGTAAC GGCGATAGAA ATTGATTCTA AATTATGTGA GGTAACTCGT AATAAGCTCT
     1681 TAAATTATCC TAACTATCAA ATAGTAAATG ATGATATACT GAAATTTACA TTTCCTAGCC
     1741 ACAATCCATA TAAAATATTT GGCAGCATAC CTTACAACAT AAGCACAAAT ATAATTCGAA
     1801 AAATTGTTTT TGAAAGTTCA GCCACAATAA GTTATTTAAT AGTGGAATAT GGTTTTGCTA
     1861 AAATGTTATT AGATACAAAC AGATCACTAG CATTGCTGTT AATGGCAGAG GTAGATATTT
     1921 CTATATTAGC AAAAATTCCT AGGTATTATT TCCATCCAAA ACCTAAAGTG GATAGCACAT
     1981 TAATTGTATT AAAAAGAAAG CCAGCAAAAA TGGCATTTAA AGAGAGAAAA AAATATGAAA
     2041 CTTTTGTAAT GAAATGGGTT AACAAAGAGT ACGAAAAACT GTTTACAAAA AATCAATTTA
     2101 ATAAAGCTTT AAAACATGCG AGAATATATG ATATAAACAA TATTAGTTTC GAACAATTTG
     2161 TATCGCTATT TAATAGTTAT AAAATATTTA ACGGCTAAAA ACAATAGGCC ACATGCAACT
     2221 GTAAATGTTT ACGCGTCCTC GGTACCAACA AATACTTTCA GGACGATGTA AAGTCCTGAA
     2281 AAATCAAAgc tggccaatga gacgacgggg tcatcacggc tcatcatgcg ccaaacaaat
     2341 gtgtgcaata cacgctcgga tgactgcatg atgaccgcac tgactgggga cagcagatcc
     2401 acctaagcct gtgagagaag cagacacccg acagatcaag gcagttaact agtgcactgc
     2461 agtggcgata aaacgaaagg ctcagtcgaa agactgggtc tttcgtttta caattgggct
     2521 accttttttt tgttttgttt gcaatggtta atctattgtt aaaatttaaa gtttcacttg
     2581 aactttcaaa taatgttctt atatttgcag Tgtcgaaaga aacaaagtag gactgatcgt
     2641 ccatcaattt aaaatttaaa ataatgagaa atttaaaatg gttgtatgca tgctctttgg
     2701 cgatagcctt cggtgtgttg tcttttgtaa cagtttcctg tcatgatgat gacgatgaac
     2761 caaagcaaga accaggtgaa gtgattgaaa ctcccgctcc ggttgtcttc acactcgaag
     2821 atttcgttgg ggactggcga cagacagccg gctacaacct ggaccaagtc cttgaacagg
     2881 gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt aactccgatc caaaggattg
     2941 tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt catcatcccg tatgaaggtc
     3001 tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa ggtggtgtac cctgtggatg
     3061 atcatcactt taaggtgatc ctgcactatg gcacactggt aatcgacggg gttacgccga
     3121 acatgatcga ctatttcgga cggccgtatg aaggcatcgc cgtgttcgac ggcaaaaaga
     3181 tcactgtaac agggaccctg tggaacggca acaaaattat cgacgagcgc ctgatcaacc
     3241 ccgacggctc cctgctgttc cgagtaacca tcaacggagt gaccggctgg cggctgtgcg
     3301 aacgcattct ggcgGGATCT TCTggctcga gcggcGACTA CAAAGACCAT GACGGTGATT
     3361 ATAAAGATCA TGACATCGAT TACAAGGATG ACGATGACAA GtgAATAGAT CACCATTAaC
     3421 CAGGCATCAA ATAAAACGAA AGGCTCAGTC GAAAGACTGG GCCTTTCGTT TTATCTGTTG
     3481 TTTGTCGGTG AACGCTCTCt acagcggccg cgattatcaa aaaggatctt cacctagatc
     3541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3601 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3661 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3721 ggccccagtg ctgcaatgat accgcgggac ccacgctcac cggctccaga tttatcagca
     3781 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3841 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3901 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3961 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4021 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4081 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4141 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4201 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4261 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4321 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4381 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4441 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4501 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4561 ggggttccgc gcacatttcc ccgaaaagtg ccacctgtca tgaccaaaat cccttaacgt
     4621 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4681 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4741 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4801 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4861 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4921 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4981 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5041 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5101 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5161 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5221 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5281 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5341 cctgattctg tggataaccg tgcggccgcc cctCCAAAGG GTTCGTGTAG ACTTTCCTTG
     5401 GTGTATCCAA CGGCGTCAGC CGGGCAGGAT AGGTGAAGTA GGCCCACCCG CGAGCGGGTG
     5461 TTCCTTCTTC ACTGTCCCTT ATTCGCACCT GGCGGTGCTC AACGGGAATC CTGCTCTGCG
     5521 AGGCTGGCCG GCTACCGCCG GCGTAACAGA TGAGGGCAAG CGGATGGCTG ATGAAACCAA
     5581 GCCAACCAGG AAGGGCAGCC CACCTATCAA GGTGTACTGC CTTCCAGACG AACGAAGAGC
     5641 GATTGAGGAA AAGGCGGCGG CGGCCGGCAT GAGCCTGTCG GCCTACCTGC TGGCCGTCGG
     5701 CCAGGGCTAC AAAATCACGG GCGTCGTGGA CTATGAGCAC GTCCGCGAGC TGGCCCGCAT
     5761 CAATGGCGAC CTGGGCCGCC TGGGCGGCCT GCTGAAACTC TGGCTCACCG ACGACCCGCG
     5821 CACGGCGCGG TTCGGTGATG CCACGATCCT CGCCCTGCTG GCGAAGATCG AAGAGAAGCA
     5881 GGACGAGCTT GGCAAGGTCA TGATGGGCGT GGTCCGCCCG AGGGCAGAGC CATGACTTTT
     5941 TTAGCCGCTA AAACGGCCGG GGGGTGCGCG TGATTGCCAA GCACGTCCCC ATaacgCATA
     6001 GCGCTCCCCG ACCGATGATT TTTAAGTGAC TGATTTTGTG CTGTTTTGGG GGTATATTAA
     6061 GAATAAAAGA AATAGAATAA GTTAAGTACT TGATACACAA TATAGGGCAT TTTCCATATT
     6121 GGAAATTCTC ATTTTCCAAT CTAGAAAATA CCGATTTCCT AATATGATAC TAAATAGGAA
     6181 AATAATATTT CCCTTAATAT TGTTTTTATG GAAAATAATA GTTTACTTTG TGGAGAATAA
     6241 TATTTCCCAA AAACATATCA AAATGGAAAA TAAAAAAGCA GTTAAGTTAA CCGATTTTCA
     6301 AAAGAACGAA GAAAATCCTT TTATGAAACA AGCTATAGAA GGTATTGAAA ATCATGTTGT
     6361 TAAAAAGTAT AAGAGTAATA GTGGTGGCGA TAAGAGAGCT GTAGTAGCTT TAGCCGACAC
     6421 TGAAACTGGA GAAGTGTTTA AGACTTCGTT TATCCGTCAA ATAGAAGTAG ATGAAGAACA
     6481 ATTCACTAAA TTGTATCTTT CTAACTTTGC TGCATTCTTT GACCTATCAC AAGCAGCTAT
     6541 TCGGGTTTTT GGTTACTTTA TGACCTGCAT GAAACCCAAA AATGATTTAA TCATTTTCAA
     6601 TAGAAAAAAA TGCCTAGAAT ATACCAAATA CAAAACAGAC AAAGCCGTTT ATAAAGGACT
     6661 TGCAGAACTT GTAAAAGCTG AAATCATAGC CCGAGGACCA GCCGATAATC TTTGGTTTAT
     6721 TAATCCTCTG ATAGTATTCA ATGGTGACCG AGTGACATTT GCTAAAACAT ACGTTCGGAA
     6781 AAAGACTTTA GCTGCCCAAA AGAAAGAAGA AGCAGAGAAA CGACAATTAT CACTTGGCTT
     6841 TGATGAACAG TAACACTCCA TTGAGTGAAG CTGCCGTTTG GTCGCTCCCC CTTTGGGGGC
     6901 GGGGGGGGAT AGATAAAGTT CCTCTATGTA AAGTTATAAT GGGGGATGAA AGGCAAGGTT
     6961 CGCTAACCTT ACCCCGATTG TTTATGCTTC CCAGCATTCA ATCTATCCCC GTTTATGGGT
     7021 TTCTCTTTCA CCCTACAAAG ATAACCTCAT GGGGGAAAAA TGTCCAAGAA TATGGGGTAA
     7081 AAACTATCAA GTCGGTAGAA AATAAGTATC TTTGAAGTAC ATTTTTAGTA GAGGTACTGG
     7141 TGTGCCTAGG CGAAGCAGAT AGGCAAAAAT AAAGCCGCTG GAGATGTCGG ATGTCCGTCA
     7201 ATTAACTCCA GCGGCTTGTA TTTTGGTAAC TCTCCCGTAT CGGTCAGCTA CCGTTTGGTC
     7261 AGCAACAAAG GTAGTACTTT ATTATCGCAA ATCCAAATGT TTTTCCCTTT ATTTGTGCAT
     7321 TTACAAAACA TTCACTAACT TCGCACCTAT GCAGTATGGT GATAGCACAC TAGCACCCTA
     7381 AAGAGTGCGT GTGTAAGGGG ACAAGCCCCC TTAACCCCCT GTCAGCCGGC TGTCACCGGC
     7441 TGTTAT
//