LOCUS       pYHY3                   8402 bp ds-DNA     circular     01-FEB-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    169..757
                     /label="pBR322 ori"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     oriT            917..1026
                     /label="oriT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1659..2348
                     /label="RepA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3051..4154
                     /label="mobA"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    4405..5139
                     /label="ermG"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(5448..6071)
                     /label="TetR"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: tetR from transposon Tn10 note: TetR binds to the tetracycline operator tetO to inhibit transcription. This inhibition can be relieved by adding tetracycline or doxycycline. product: tetracycline repressor TetR protein_id: transl_table: 1 translation: MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS*"
     misc_feature    complement(6072..6371)
                     /label="BT1311 promoter and RBS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6374..6584
                     /label="P1T_DP-A21"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     protein_bind    6461..6479
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR gene: tetO note: bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6523..6541
                     /label="tet operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: tetracycline repressor TetR gene: tetO note: bacterial operator O2 for the tetR and tetA genes"
     misc_feature    6542..6584
                     /label="A21 RBS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6585..6701
                     /label="BT3630 SP"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             6585..6701
                     /label="Translation 6585-6701"
                     /translation="MMKKGILFVLTAAFLASCQQEENEGVASVDRVTITPIIT"
     misc_feature    6702..7211
                     /label="Nanoluc"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             6702..7211
                     /label="Translation 6702-7211"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILA"
     CDS             7212..7232
                     /label="Translation 7212-7232"
                     /translation="GSSGSSG"
     misc_feature    7212..7232
                     /label="flexible linker"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    7233..7298
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             7233..7298
                     /label="Translation 7233-7298"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             7299..7301
                     /label="Translation 7299-7301"
                     /translation="*"
     terminator      7325..7396
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(7503..8363)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
       61 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
      121 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
      181 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
      241 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
      301 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
      361 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
      421 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
      481 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
      541 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
      601 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
      661 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
      721 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
      781 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
      841 ttctgtggat aaccgtgcgg ccgcccctCC AAAGGGTTCG TGTAGACTTT CCTTGGTGTA
      901 TCCAACGGCG TCAGCCGGGC AGGATAGGTG AAGTAGGCCC ACCCGCGAGC GGGTGTTCCT
      961 TCTTCACTGT CCCTTATTCG CACCTGGCGG TGCTCAACGG GAATCCTGCT CTGCGAGGCT
     1021 GGCCGGCTAC CGCCGGCGTA ACAGATGAGG GCAAGCGGAT GGCTGATGAA ACCAAGCCAA
     1081 CCAGGAAGGG CAGCCCACCT ATCAAGGTGT ACTGCCTTCC AGACGAACGA AGAGCGATTG
     1141 AGGAAAAGGC GGCGGCGGCC GGCATGAGCC TGTCGGCCTA CCTGCTGGCC GTCGGCCAGG
     1201 GCTACAAAAT CACGGGCGTC GTGGACTATG AGCACGTCCG CGAGCTGGCC CGCATCAATG
     1261 GCGACCTGGG CCGCCTGGGC GGCCTGCTGA AACTCTGGCT CACCGACGAC CCGCGCACGG
     1321 CGCGGTTCGG TGATGCCACG ATCCTCGCCC TGCTGGCGAA GATCGAAGAG AAGCAGGACG
     1381 AGCTTGGCAA GGTCATGATG GGCGTGGTCC GCCCGAGGGC AGAGCCATGA CTTTTTTAGC
     1441 CGCTAAAACG GCCGGGGGGT GCGCGTGATT GCCAAGCACG TCCCCATaac gCATAGCGCT
     1501 CCCCGACCGA TGATTTTTAA GTGACTGATT TTGTGCTGTT TTGGGGGTAT ATTAAGAATA
     1561 AAAGAAATAG AATAAGTTAA GTACTTGATA CACAATATAG GGCATTTTCC ATATTGGAAA
     1621 TTCTCATTTT CCAATCTAGA AAATACCGAT TTCCTAATAT GATACTAAAT AGGAAAATAA
     1681 TATTTCCCTT AATATTGTTT TTATGGAAAA TAATAGTTTA CTTTGTGGAG AATAATATTT
     1741 CCCAAAAACA TATCAAAATG GAAAATAAAA AAGCAGTTAA GTTAACCGAT TTTCAAAAGA
     1801 ACGAAGAAAA TCCTTTTATG AAACAAGCTA TAGAAGGTAT TGAAAATCAT GTTGTTAAAA
     1861 AGTATAAGAG TAATAGTGGT GGCGATAAGA GAGCTGTAGT AGCTTTAGCC GACACTGAAA
     1921 CTGGAGAAGT GTTTAAGACT TCGTTTATCC GTCAAATAGA AGTAGATGAA GAACAATTCA
     1981 CTAAATTGTA TCTTTCTAAC TTTGCTGCAT TCTTTGACCT ATCACAAGCA GCTATTCGGG
     2041 TTTTTGGTTA CTTTATGACC TGCATGAAAC CCAAAAATGA TTTAATCATT TTCAATAGAA
     2101 AAAAATGCCT AGAATATACC AAATACAAAA CAGACAAAGC CGTTTATAAA GGACTTGCAG
     2161 AACTTGTAAA AGCTGAAATC ATAGCCCGAG GACCAGCCGA TAATCTTTGG TTTATTAATC
     2221 CTCTGATAGT ATTCAATGGT GACCGAGTGA CATTTGCTAA AACATACGTT CGGAAAAAGA
     2281 CTTTAGCTGC CCAAAAGAAA GAAGAAGCAG AGAAACGACA ATTATCACTT GGCTTTGATG
     2341 AACAGTAACA CTCCATTGAG TGAAGCTGCC GTTTGGTCGC TCCCCCTTTG GGGGCGGGGG
     2401 GGGATAGATA AAGTTCCTCT ATGTAAAGTT ATAATGGGGG ATGAAAGGCA AGGTTCGCTA
     2461 ACCTTACCCC GATTGTTTAT GCTTCCCAGC ATTCAATCTA TCCCCGTTTA TGGGTTTCTC
     2521 TTTCACCCTA CAAAGATAAC CTCATGGGGG AAAAATGTCC AAGAATATGG GGTAAAAACT
     2581 ATCAAGTCGG TAGAAAATAA GTATCTTTGA AGTACATTTT TAGTAGAGGT ACTGGTGTGC
     2641 CTAGGCGAAG CAGATAGGCA AAAATAAAGC CGCTGGAGAT GTCGGATGTC CGTCAATTAA
     2701 CTCCAGCGGC TTGTATTTTG GTAACTCTCC CGTATCGGTC AGCTACCGTT TGGTCAGCAA
     2761 CAAAGGTAGT ACTTTATTAT CGCAAATCCA AATGTTTTTC CCTTTATTTG TGCATTTACA
     2821 AAACATTCAC TAACTTCGCA CCTATGCAGT ATGGTGATAG CACACTAGCA CCCTAAAGAG
     2881 TGCGTGTGTA AGGGGACAAG CCCCCTTAAC CCCCTGTCAG CCGGCTGTCA CCGGCTGTTA
     2941 TCAATCTTCA AAGTCGGGTG GTTGTCAAGA TGATTTTTTT GGTTTGGTGT CGTCTTTTTT
     3001 TAAGCTGCCG CATAACGGTT TGGCGCCGAT TGTCACAAAA ATGTTAACTT ATGGGAGCAA
     3061 CAAGTATTCA TGTACAAGCA GTGAAGCCGG GGAGCGAGAT TCACAACTTT AGGGAAAAAG
     3121 AGTTGGACTA TGTTCGTCCC GAACTTAGTC ATTTGAATGA AAGCTGGGTT GGAGATAGCA
     3181 TTTCCCATCG GCTGGAGAGT GCAAAACAAA GATATCTCGA TACGGTTGGG CAGAAGATGC
     3241 AGGCTAAAGC CGCACCCATA CGAGAGGGAG TAATAGTAAT CAAACAAGAA ACCACTATGC
     3301 AGGAACTCCA GCAGTTTGCC ACGGTCTCCA AAGAACGTTT CGGTATCGAA GCGTTTCAAA
     3361 TCCATATACA CAAGGACGAA GGATACATGA ACGCAAAGCA GTGGACACCT AACCTTCATG
     3421 CCCATGTAGT TTTCGATTGG ACGCAGCCGA ATGGGAAAAG TGTGCGTTTA TCGCGTGATG
     3481 ACATGGCAGA ACTCCAAACC ATAGCATCTG AAACACTGGG CATGGAACGT GGCGTTTCTT
     3541 CTGACCGCAA ACACTTATCG GCTATGCAGT ACAAGACCGA ATGTGCGAAA GAACAGCTTC
     3601 AGGAACTATC CAACGATATA TCCAGTGCAT TAGACAAATA CAAGGACGCA CAAAACCAGC
     3661 TTCTTCAGCT TCAGAAGGAA CTACGTTCCA TTGAAACAAA GAAGAACGTC CAAAAACTCA
     3721 TTTCTAAGGC TTCAGAGAAG TTTTACGGCT TGATTGGAAA AACAGTCAAC GATAGGGAAA
     3781 AGGATGCCTT AAAAGCCAAA GTAAAGACTT TAGAGGGGGA AAATGAACAA CTATCCGATA
     3841 GACTGGGAAA GGCTATACTT GAAAAAGAGC GAAACAGCAC AAAGGCATTC AAAGCCGAGA
     3901 ATGACAAGGA GTATTATAGA CAACAAATGG ACAACGCAAG GACAACCAGT AACCTATTAA
     3961 GGACGGAGAA CCAAAAACTA AAAGCCGAAA CCAAGGAGTT GAAAAAAGAA CTTGGTAAGA
     4021 TGAAAGACTT GTTCAATTCC GAACAACTGG AGGCTTTAAG GCATCATTTC CCAAACATAT
     4081 CAAAAGCTAT GGAAGAGGGG AAAGACCTAT TCAAGCAAAT CACCAGAAGC AGAGGTTTCG
     4141 GCATGGGTAT GTAACCCCCC CCCGCCCCCA AAGGGGGAGC GACCAAACGG CAGCCTCTCT
     4201 CAATGGAGTG TTACatccCG TAAAGAGGGG CTTTGCAGGG GGTGGACTCA GAAAGATGAG
     4261 AATAGATGAC TATTGTAGTT GAAACACATA GAAAGTTGCT GATATACAGA CCGATACGCA
     4321 TATCGGGATG AACCATGAGT ACGTTCTTTT CTCAAAAAAC ATAAATATTC GAAAAGAGAT
     4381 GCAATAAATT AAGGAGAGGT TATAATGAAC AAAGTAAATA TAAAAGATAG TCAAAATTTT
     4441 ATTACTTCAA AATATCACAT AGAAAAAATA ATGAATTGCA TAAGTTTAGA TGAAAAAGAT
     4501 AACATCTTTG AAATAGGTGC AGGGAAAGGT CATTTTACTG CTGGATTGGT AAAGAGATGT
     4561 AATTTTGTAA CGGCGATAGA AATTGATTCT AAATTATGTG AGGTAACTCG TAATAAGCTC
     4621 TTAAATTATC CTAACTATCA AATAGTAAAT GATGATATAC TGAAATTTAC ATTTCCTAGC
     4681 CACAATCCAT ATAAAATATT TGGCAGCATA CCTTACAACA TAAGCACAAA TATAATTCGA
     4741 AAAATTGTTT TTGAAAGTTC AGCCACAATA AGTTATTTAA TAGTGGAATA TGGTTTTGCT
     4801 AAAATGTTAT TAGATACAAA CAGATCACTA GCATTGCTGT TAATGGCAGA GGTAGATATT
     4861 TCTATATTAG CAAAAATTCC TAGGTATTAT TTCCATCCAA AACCTAAAGT GGATAGCACA
     4921 TTAATTGTAT TAAAAAGAAA GCCAGCAAAA ATGGCATTTA AAGAGAGAAA AAAATATGAA
     4981 ACTTTTGTAA TGAAATGGGT TAACAAAGAG TACGAAAAAC TGTTTACAAA AAATCAATTT
     5041 AATAAAGCTT TAAAACATGC GAGAATATAT GATATAAACA ATATTAGTTT CGAACAATTT
     5101 GTATCGCTAT TTAATAGTTA TAAAATATTT AACGGCTAAA AACAATAGGC CACATGCAAC
     5161 TGTAAATGTT TACGCGTCCT CGGTACCAAC AAATACTTTC AGGACGATGT AAAGTCCTGA
     5221 AAAATCAAAg ctggccaatg agacgacggg gtcatcacgg ctcatcatgc gccaaacaaa
     5281 tgtgtgcaat acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc
     5341 cacctaagcc tgtgagagaa gcagacaccc gacagatcaa ggcagttaac tagtgcactg
     5401 cagtggcctt aacggctgac atgggaattc ccctccaccg cggtggctta agacccactt
     5461 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
     5521 ggctctgcac cttggtgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
     5581 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgatc ttccaatacg
     5641 caacctaaag taaaatgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
     5701 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
     5761 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
     5821 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
     5881 tggtgcctat ctaacatctc aatggctaag gcgtcgagca aagcccgctt attttttaca
     5941 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
     6001 ccttcgattc cgacctcatt aagcagctct aatgcgctgt taatcacttt acttttatct
     6061 aatctagaca ttctttaaaa acagatttgg agtgcaaagt tacgacaaat aatttgttaa
     6121 catacatatt taggcaactt tttcactata ttttcaacta ataaaacaaa aaagaatgcc
     6181 gacaagttgt gtgacggcat cctttttcta tttcattagc ttatcctaca atcttcttac
     6241 cttaatttat aatttattcc tgagtcagat caactgcaaa gtatccgcgt ttaccggatg
     6301 ggaatacacc tgtcaggaac aatacctgat tcggagactt aacagcagct ttcattgctt
     6361 cttccagatc atttttgcac ccgctttcca agagaagaaa gccttgttaa attgacttag
     6421 tgtaaaagcg cagtactgct tgaccataag aacaaaaaaa tctctatcac tgatagggat
     6481 aaagtttgga agataaagct aaaagttctt atctttgcag tctccctatc agtgatagag
     6541 acgcatttta aaataaaata aattatttat gatattaaac gaatatgatg aaaaagggga
     6601 tattatttgt gctgacagca gcatttctgg catcttgcca gcaggaggaa aacgagggag
     6661 tagcatctgt ggaccgggtg accatcactc ctattatcac agtcttcaca ctcgaagatt
     6721 tcgttgggga ctggcgacag acagccggct acaacctgga ccaagtcctt gaacagggag
     6781 gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac tccgatccaa aggattgtcc
     6841 tgagcggtga aaatgggctg aagatcgaca tccatgtcat catcccgtat gaaggtctga
     6901 gcggcgacca aatgggccag atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc
     6961 atcactttaa ggtgatcctg cactatggca cactggtaat cgacggggtt acgccgaaca
     7021 tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
     7081 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcaaccccg
     7141 acggctccct gctgttccga gtaaccatca acggagtgac cggctggcgg ctgtgcgaac
     7201 gcattctggc gGGATCTTCT ggctcgagcg gcGACTACAA AGACCATGAC GGTGATTATA
     7261 AAGATCATGA CATCGATTAC AAGGATGACG ATGACAAGtg AATAGATCAC CATTAaCCAG
     7321 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT
     7381 GTCGGTGAAC GCTCTCtaca gcggccgcga ttatcaaaaa ggatcttcac ctagatcctt
     7441 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     7501 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7561 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7621 cccagtgctg caatgatacc gcgggaccca cgctcaccgg ctccagattt atcagcaata
     7681 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7741 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7801 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7861 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7921 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7981 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     8041 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     8101 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     8161 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     8221 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     8281 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     8341 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     8401 gg
//