LOCUS       lenti-ER-mTagBFP2       9026 bp ds-DNA     circular     29-JAN-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      574..708
                     /label="SV40 ori"
     promoter        770..817
                     /label="EM7 promoter"
     CDS             836..1210
                     /label="BleoR"
     polyA_signal    1340..1461
                     /label="SV40 poly(A) signal"
     primer_bind     complement(1510..1526)
                     /label="M13 rev"
     protein_bind    1534..1550
                     /label="lac operator"
     promoter        complement(1558..1564)
                     /label="lac promoter"
     promoter        complement(1565..1582)
                     /label="lac promoter"
     promoter        complement(1583..1588)
                     /label="lac promoter"
     protein_bind    1603..1624
                     /label="CAP binding site"
     rep_origin      complement(1912..2500)
                     /label="ori"
     CDS             complement(2671..3462)
                     /label="AmpR"
     CDS             complement(3463..3531)
                     /label="AmpR"
     promoter        complement(3532..3636)
                     /label="AmpR promoter"
     enhancer        3902..4281
                     /label="CMV enhancer"
     promoter        4283..4481
                     /label="CMV promoter"
     LTR             4499..4679
                     /label="5' LTR (truncated)"
     misc_feature    4726..4851
                     /label="HIV-1 Ψ"
     misc_feature    5344..5577
                     /label="RRE"
     misc_feature    6104..6221
                     /label="cPPT/CTS"
     promoter        6314..6525
                     /label="EF-1α core promoter"
     primer          6454..6474
                     /label="EF_1_alpha_F"
                     /note="sequence: ggagaaccgtatataagtgca"
     primer          6513..6613
                     /label="BFP_barcoding_6_fwd"
                     /note="sequence: cgccagaacacaggaccggtgccaccatggacagcaaaggttcgtcgcagaaagggtcccgcctgctcctgctgctggtggtgtcaaatctactcttgtgc"
     regulatory      6533..6538
                     /label="Kozak sequence"
     misc_feature    6539..6634
                     /label="Prolactin signal sequence"
     CDS             6539..7358
                     /label="Translation 6539-7358"
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVSTGVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDLPSKLGHKLNKDEL*"
     primer          6587..6661
                     /label="BFP_barcoding_5_fwd"
                     /note="sequence: ctggtggtgtcaaatctactcttgtgccagggtgtggtctccaccggagtgtctaagggcgaagagctgattaag"
     misc_feature    6635..7342
                     /label="mtagBFP2"
     primer          complement(7317..7377)
                     /label="BFP_barcoding_7_rev"
                     /note="sequence: ataagcttgatatcgaattcttacagctcgtccttattaagcttgtgccccagtttgctag"
     misc_feature    7343..7353
                     /label="KDEL"
     misc_feature    7382..7382
                     /label="WPRE"
     misc_feature    7383..7970
                     /label="WPRE"
     CDS             complement(7853..7864)
                     /label="Factor Xa site"
     primer_bind     complement(7973..7989)
                     /label="KS primer"
     LTR             8495..8675
                     /label="5' LTR (truncated)"
     polyA_signal    8707..8931
                     /label="bGH poly(A) signal"
     rep_origin      8977..379
                     /label="f1 ori"
ORIGIN
        1 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
       61 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      121 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      181 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      241 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      301 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      361 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
      421 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
      481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      541 cagcaaccat agTCCCGCCC CTAACTCCGC CCAtcccgcc cctaactccg cccagttccg
      601 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
      661 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
      721 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
      781 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
      841 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
      901 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
      961 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     1021 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     1081 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     1141 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     1201 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1261 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1321 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1381 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1441 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1501 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1561 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1621 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1681 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3421 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3601 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3661 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     3721 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3781 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     3841 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     3901 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3961 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4021 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4081 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4141 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4201 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4261 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4321 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4381 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4441 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     4501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4681 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     4741 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     4801 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     4861 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     4921 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     4981 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     5041 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     5101 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     5161 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     5221 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     5281 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     5341 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     5401 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     5461 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     5521 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     5581 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     5641 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     5701 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     5761 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     5821 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     5881 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     5941 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     6001 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     6061 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     6121 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     6181 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     6241 gcagagatcc agtttggtta attagctagc taggtcttga aaggagtggg aattggctcc
     6301 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6361 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6421 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6481 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg gtgccaccat
     6541 ggacagcaaa ggttcgtcgc agaaagggtc ccgcctgctc ctgctgctgg tggtgtcaaa
     6601 tctactcttg tgccagggtg tggtctccac cggagtgtct aagggcgaag agctgattaa
     6661 ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc acttcaagtg
     6721 cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggtggt
     6781 cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc tctacggcag
     6841 caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt ccttccctga
     6901 gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga ccgctaccca
     6961 ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt
     7021 cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct tcaccgagac
     7081 gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga agctcgtggg
     7141 cgggagccat ctgatcgcaa acgccaagac cacatataga tccaagaaac ccgctaagaa
     7201 cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca aggaggccaa
     7261 caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg acctccctag
     7321 caaactgggg cacaagctta ataaggacga gctgtaagaa ttcgatatca agcttatcgg
     7381 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     7441 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     7501 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     7561 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     7621 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     7681 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     7741 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     7801 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     7861 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     7921 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     7981 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     8041 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     8101 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8161 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     8221 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     8281 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     8341 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     8401 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     8461 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     8521 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8581 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8641 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     8701 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     8761 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     8821 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     8881 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     8941 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     9001 gcgcggcggg tgtggtggtt acgcgc
//