LOCUS       pBi2FC-N-MPK8-P2       13503 bp DNA     circular SYN 22-FEB-2023
FEATURES             Location/Qualifiers
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1083
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1165..1683
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
     CDS             1630..1683
                     /codon_start=1
                     /label=Common Venus Fragment
     CDS             1684..1713
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
     protein_bind    1732..1756
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1768..3534
                     /codon_start=1
                     /label=MPK8
     protein_bind    complement(3545..3569)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3577..3633
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     CDS             3634..3654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     CDS             3655..3675
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     CDS             3676..3696
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     CDS             3697..4407
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
     terminator      4424..4676
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4710..4726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4734..4750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4758..4788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4803..4824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      4860..5112
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5263..6288)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
     promoter        complement(6340..6519)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7025..7049
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7570..8361
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
     rep_origin      8607..9195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9381..9521
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      9865..10059
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10125..11198)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11627..12256)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac
      661 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
      721 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      781 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      841 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
      901 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      961 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     1021 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     1081 tgaaatcacc agtgtatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
     1141 caattactat ttacaatttc tagaatggtg agcaagggcg aggagctgtt caccggggtg
     1201 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     1261 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
     1321 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
     1381 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1441 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1501 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1561 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1621 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
     1681 gaggagcaaa agttgatttc tgaggaggat cttgggccca ggcctactag tacaagtttg
     1741 tacaaaaaag caggctccgc ggccgcaatg ggtggtggtg ggaatctcgt cgacggtgtt
     1801 cgtcgatggc tttttcagcg accttcttct tcttcttctt cttcttcttc caacaataat
     1861 aataataatc acgaacaacc cattttcaat tcttcttctt tctcttcttc ctctaacccc
     1921 aatcattccg ctaactctgg agagcttatt attgaagaag atctcgattt ctctggtcta
     1981 acgcttatta acgttcctaa acgtaatcat ttacctatgg atcctcacaa gaagggtgaa
     2041 actgaattct tcacggagta tggagaagct aacaggtatc agattcaaga ggttgttggc
     2101 aaaggaagtt atggtgttgt ggcttctgct gttgactcac acactggaga gagagttgcg
     2161 atcaagaaga tcaacgatgt gtttgagcat gtctccgatg ctactaggat tctcagagag
     2221 atcaaattgc ttcggttgct tcgtcatcct gatgttgtgg agattaaaca catcatgtta
     2281 cctccttctc gcagagaatt ccgcgatatt tatgttgtct ttgagttgat ggaatctgat
     2341 cttcatcaag tgatcaaggc gaatgatgat ttgactcctg agcattatca gtttttcttg
     2401 taccagcttc ttcgtggtct gaaatatgtt cacgcagcta atgtgtttca tcgggatttg
     2461 aaaccgaaga acattctagc taatgctgat tgcaaattga agatctgtga ttttgggctg
     2521 gctcgcgttt cttttaacga tgccccaacc gctatatttt ggactgatta tgtagctact
     2581 cggtggtatc gtgcccctga actctgtgga tcgtttttct ccaaatatac ccctgcgatt
     2641 gatatctgga gtgtcggttg catttttgcg gaaatgcttt tgggtaagcc tttgtttccc
     2701 gggaaaaacg ttgtgcacca attggatcta atgactgact ttcttggtac tccacctcct
     2761 gagtcaatat caaggattag aaatgaaaag gcgaggagat atcttagcag catgaggaaa
     2821 aaacaaccag tccctttctc tcataaattc cctaaagctg atcctttggc tctccgcctt
     2881 ctggaacgcc tgcttgcctt tgatccgaaa gatcgtgcat cagctgaaga tgcactagct
     2941 gatccatatt tcagtggttt gtcaaattca gagcgtgaac caacaacaca gccaatttca
     3001 aagcttgaat ttgactttga gagaaagaag ttagttaaag atgatgtcag agaattaatt
     3061 taccgagaga tattggaata tcatcctcag atgttggagg aataccttcg tggtggtgat
     3121 cagcttagct ttatgtaccc aagtggggta gaccgattta aaaggcaatt tgctcatctt
     3181 gaagaaaatc aaggtaaacc aggagcagca ggaggaggaa gaagtactgc acttcacaga
     3241 catcatgctt ccttgccaag agagagagtt cctgctccaa atggtgaaac tgcagaagaa
     3301 agcagtgatg ttgagagaag agcagcagct gctgtggctt caaccttgga atccgaggaa
     3361 gcagacaatg gaggaggtta cagtgctcgt aacctcatga agagtgcaag catcagcggt
     3421 tctaaatgca ttggtgtcca atctaaaact gacaaagagg ataccatagc tgaggaagaa
     3481 gataatgaaa cagttgcaga gcttactgat aaagttgctt ctcttcacaa ttctaggcgc
     3541 gccgacccag ctttcttgta caaagtggtt ctcgaggcaa ccaactttag cctgctcaag
     3601 caagcaggag atgttgagga aaatcctggc ccccctaaga aaaagagaaa ggttccgaaa
     3661 aagaaaagaa aggtgccaaa aaaaaagaga aaggtaatgg tgagcaaggg cgaggaggat
     3721 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     3781 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3841 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     3901 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
     3961 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     4021 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     4081 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     4141 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     4201 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     4261 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     4321 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     4381 ggcggcatgg acgagctgta caagtaagag ctcgaatttc cccgatcgtt caaacatttg
     4441 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4501 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4561 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4621 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     4681 attggttccg gaaccaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4741 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4801 taatgagtga gctaactcac attaggctga attaggcgcg cctatttcta ttaattcccg
     4861 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
     4921 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
     4981 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
     5041 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
     5101 gtttgaacga tcggggaaat tcgggggatc gatcccgcga actgtggacg agaactgtgc
     5161 caccaagcgt aaggccgttc tctcgcattt gccttgctag gctcgcgcga gttgctggct
     5221 gaggcgttct cgaaatcagc tcttgttcgg tcggcatcta ctctattcct ttgccctcgg
     5281 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca
     5341 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac
     5401 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct
     5461 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc
     5521 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg
     5581 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca
     5641 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc
     5701 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc
     5761 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg
     5821 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc
     5881 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc
     5941 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc
     6001 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aatgccccaa tgtcaagcac
     6061 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa
     6121 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa
     6181 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc
     6241 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat cttattgccc
     6301 cccgggatca gatctggatt gagagtgaat atgagactct aattggatac cgaggggaat
     6361 ttatggaacg tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta
     6421 ggcgactttt gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca
     6481 gaaacccgcg gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac
     6541 ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga
     6601 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
     6661 gggcttccca accttaccag agggcgcccc agctggcaat tccggttcgc ttgctgtcca
     6721 taaaaccgcc cagtctagct atcgccatgt aagcccactg caagctacct gctttctctt
     6781 tgcgcttgcg ttttcccttg tccagatagc ccagtagctg acattcatcc ggggtcagca
     6841 ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt tagcagccct tgcgccctga
     6901 gtgcttgcgg cagcgtgaag ctgatccgtc gagattaata gaaataaatt cagcctaatt
     6961 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
     7021 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
     7081 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
     7141 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
     7201 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
     7261 atgacagagc gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctgt
     7321 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     7381 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     7441 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga
     7501 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag
     7561 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt
     7621 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca
     7681 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg
     7741 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct
     7801 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt
     7861 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt
     7921 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa
     7981 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt
     8041 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc
     8101 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca
     8161 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg
     8221 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat
     8281 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc
     8341 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc
     8401 ggcgcggctt aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat
     8461 caggtcaagt ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga
     8521 gatatatcat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     8581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     8761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     9301 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     9361 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     9421 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9481 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
     9541 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     9601 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
     9661 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
     9721 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
     9781 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
     9841 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
     9901 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
     9961 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
    10021 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc ttttcgacct
    10081 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
    10141 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
    10201 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
    10261 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
    10321 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
    10381 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
    10441 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
    10501 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
    10561 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
    10621 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
    10681 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
    10741 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
    10801 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
    10861 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
    10921 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
    10981 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
    11041 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
    11101 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
    11161 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
    11221 ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg
    11281 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
    11341 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
    11401 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
    11461 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
    11521 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
    11581 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
    11641 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
    11701 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
    11761 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
    11821 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
    11881 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
    11941 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
    12001 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
    12061 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
    12121 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
    12181 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
    12241 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
    12301 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
    12361 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
    12421 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
    12481 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
    12541 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
    12601 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
    12661 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
    12721 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
    12781 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
    12841 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    12901 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    12961 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    13021 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    13081 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    13141 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    13201 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    13261 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    13321 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    13381 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    13441 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    13501 cct
//
