LOCUS       pBi2FC-C-GRF4-P2       12273 bp DNA     circular SYN 25-JUN-2024
FEATURES             Location/Qualifiers
     source          1..12273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1083
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1168..1419
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             1168..1221
                     /codon_start=1
                     /label=Common Venus Fragment
                     /translation="DKQKNGIKANFKIRHNIE"
     CDS             1420..1446
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1465..1489
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1501..2301
                     /codon_start=1
                     /label=GRF4_Isoform1
                     /translation="MAAPPASSSAREEFVYLAKLAEQAERYEEMVEFMEKVAEAVDKDE
                     LTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNDDHVTTIRDYRSKIESELSK
                     ICDGILKLLDTRLVPASANGDSKVFYLKMKGDYHRYLAEFKTGQERKDAAEHTLTAYKA
                     AQDIANAELAPTHPIRLGLALNFSVFYYEILNSPDRACNLAKQAFDEAIAELDTLGEES
                     YKDSTLIMQLLRDNLTLWTSDMQDESPEEIKEAAAPKPAEEQKEI"
     protein_bind    complement(2312..2336)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2344..2400
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 polyprotein
                     got from 35s_RUBY
                     "
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2401..2421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen "
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2422..2442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2443..2463
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2464..3177
                     /codon_start=1
                     /label=mTagBFP2_Full
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    2743..2745
                     /label=TGT>TGG
     misc_feature    3154..3177
                     /label=KL GHKLN From Fabio
     terminator      3194..3446
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3480..3496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3504..3520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3528..3534,3535..3552,3553..3558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3573..3594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      3630..3882
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4033..5058)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5110..5289)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5795..5819
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6340..7131
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7377..7965
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8151..8291
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      8635..8829
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8895..9968)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10397..11026)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac
      661 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
      721 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      781 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      841 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
      901 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      961 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     1021 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     1081 tgaaatcacc agtgtatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
     1141 caattactat ttacaatttc tagaatggac aagcagaaga acggcatcaa ggccaacttc
     1201 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     1261 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     1321 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1381 gccgccggga tcactctcgg catggacgag ctgtacaagt acccatacga tgttccagat
     1441 tacgctgggc ccaggcctac tagtacaagt ttgtacaaaa aagcaggctc cgcggccgca
     1501 atggcggcac caccagcatc atcctcggcg agggaagagt tcgtgtacct cgcaaagctc
     1561 gcagagcaag cggaacgtta cgaagaaatg gttgaattca tggaaaaagt cgctgaagcc
     1621 gttgacaaag acgaactcac cgtcgaagaa cgtaatctcc tctccgtcgc ttacaaaaac
     1681 gtaatcggcg ctcgtcgtgc ttcctggaga atcatctctt ccattgaaca aaaagaagag
     1741 agtcgtggta acgatgacca tgtgaccacg atccgtgatt acagaagcaa gatcgaatct
     1801 gagttatcga aaatctgtga cggtattctt aagcttcttg atactagact tgttcctgct
     1861 tctgctaatg gagattctaa ggttttttac cttaagatga agggagatta tcataggtat
     1921 ttggctgagt ttaagactgg tcaagagagg aaagatgctg ctgaacatac tctcaccgct
     1981 tacaaagctg ctcaggatat tgctaatgct gaattggctc caacgcatcc gattcgtctt
     2041 ggtttggctt tgaatttctc tgtgttttac tatgagattc ttaattctcc agatcgtgct
     2101 tgtaatctcg ctaagcaggc gtttgatgaa gccattgctg agttagatac tcttggagag
     2161 gagtcataca aggatagtac cttgatcatg cagcttcttc gtgacaatct tactctctgg
     2221 acttctgaca tgcaggacga aagtccggag gagattaaag aagcagcagc accaaagcct
     2281 gctgaagaac agaaggagat caggcgcgcc gacccagctt tcttgtacaa agtggttctc
     2341 gaggcaacca actttagcct gctcaagcaa gcaggagatg ttgaggaaaa tcctggcccc
     2401 cctaagaaaa agagaaaggt tccgaaaaag aaaagaaagg tgccaaaaaa aaagagaaag
     2461 gtaatggtga gcaagggcga ggagcttatc aaagagaata tgcacatgaa gttgtatatg
     2521 gagggtactg tggataacca tcactttaaa tgtacatcag aaggagaggg aaagccttac
     2581 gagggtactc aaacaatgag aattaaggtt gtggagggtg gtcctcttcc atttgctttc
     2641 gatatcttgg caacatcttt tctctacggt tcaaaaactt tcattaacca tacacagggt
     2701 attccagatt tcttcaagca gtcttttcca gagggattca cttgggaaag agttactact
     2761 tatgaggatg gtggtgttct tactgcaact caagatacat ctcttcagga tggatgcttg
     2821 atctataacg ttaagatcag aggagttaat ttcacttcaa acggacctgt tatgcaaaag
     2881 aaaacattgg gttgggaggc ttttactgag acattgtacc cagcagatgg aggtttggaa
     2941 ggaagaaacg atatggctct taaattggtt ggaggttctc atctcatcgc aaatgctaaa
     3001 actacatata gatcaaagaa acctgctaag aacctcaaaa tgccaggagt ttattacgtg
     3061 gattacagac ttgaaagaat taaagaggca aacaacgaaa cttatgttga acagcatgag
     3121 gttgctgtgg caagatactg tgatcttcct tctaaactgg ggcacaaact taattaagag
     3181 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     3241 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     3301 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     3361 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     3421 cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg
     3481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga
     3601 attaggcgcg cctatttcta ttaattcccg atctagtaac atagatgaca ccgcgcgcga
     3661 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg
     3721 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat
     3781 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg
     3841 attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgggggatc
     3901 gatcccgcga actgtggacg agaactgtgc caccaagcgt aaggccgttc tctcgcattt
     3961 gccttgctag gctcgcgcga gttgctggct gaggcgttct cgaaatcagc tcttgttcgg
     4021 tcggcatcta ctctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
     4081 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
     4141 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
     4201 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
     4261 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
     4321 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     4381 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     4441 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     4501 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     4561 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     4621 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     4681 atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc
     4741 ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag
     4801 gctctcgctg aatgccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa
     4861 gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac
     4921 atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg
     4981 catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt
     5041 gagttcaggc tttttcatat cttattgccc cccgggatca gatctggatt gagagtgaat
     5101 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     5161 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     5221 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     5281 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     5341 aacgtgactc ccttaattct ccgctcatga tcttgatccc ctgcgccatc agatccttgg
     5401 cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc
     5461 agctggcaat tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt
     5521 aagcccactg caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc
     5581 ccagtagctg acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt
     5641 ccgcttcctt tagcagccct tgcgccctga gtgcttgcgg cagcgtgaag ctgatccgtc
     5701 gagattaata gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc
     5761 gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac
     5821 cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca
     5881 gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca
     5941 tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat
     6001 ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt
     6061 cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt
     6121 atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg
     6181 gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc
     6241 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     6301 acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc
     6361 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
     6421 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
     6481 attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc
     6541 aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa
     6601 gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg
     6661 caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc
     6721 gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt
     6781 ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat
     6841 gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg
     6901 cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc
     6961 gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct
     7021 agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa
     7081 gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct
     7141 agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
     7201 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
     7261 cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa
     7321 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7381 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7441 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7501 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     7561 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7621 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7681 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7741 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7801 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7861 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7921 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7981 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8041 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8101 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     8161 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     8221 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
     8281 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8341 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8401 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
     8461 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
     8521 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
     8581 gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca
     8641 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
     8701 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
     8761 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
     8821 ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
     8881 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
     8941 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
     9001 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
     9061 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
     9121 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
     9181 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
     9241 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
     9301 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
     9361 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
     9421 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
     9481 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
     9541 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
     9601 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
     9661 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
     9721 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
     9781 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
     9841 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
     9901 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
     9961 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa
    10021 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    10081 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    10141 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    10201 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    10261 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    10321 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    10381 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    10441 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    10501 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    10561 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    10621 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    10681 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    10741 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    10801 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    10861 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    10921 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    10981 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    11041 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    11101 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    11161 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    11221 tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    11281 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    11341 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    11401 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    11461 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    11521 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    11581 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    11641 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    11701 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    11761 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    11821 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    11881 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    11941 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    12001 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    12061 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    12121 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    12181 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    12241 catgcacata caaatggacg aacggataaa cct
//
