LOCUS       pBi2FC-C-GW-P2A       13669 bp DNA     circular SYN 22-FEB-2023
FEATURES             Location/Qualifiers
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1083
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1168..1419
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
     CDS             1168..1221
                     /codon_start=1
                     /label=Common Venus Fragment
     CDS             1420..1446
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
     protein_bind    1465..1589
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    1905..1917
                     /label=158_better
     CDS             complement(2018..2323)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
     CDS             complement(2668..3327)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
     promoter        complement(3328..3430)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(3608..3732)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3740..3796
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 polyprotein
                     got from 35s_RUBY
                     "
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
     CDS             3797..3817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen "
                     /label=SV40 NLS
     CDS             3818..3838
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     CDS             3839..3859
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
     CDS             3860..4573
                     /codon_start=1
                     /label=mTagBFP2_Full
     misc_feature    4139..4141
                     /label=TGT>TGG
     misc_feature    4550..4573
                     /label=KL GHKLN From Fabio
     terminator      4590..4842
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4876..4892)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4900..4916
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4924..4954)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4969..4990
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      5026..5278
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5429..6454)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
     promoter        complement(6506..6685)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7191..7215
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7736..8527
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
     rep_origin      8773..9361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9547..9687
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      10031..10225
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10291..11364)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11793..12422)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac
      661 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
      721 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      781 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      841 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
      901 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      961 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     1021 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     1081 tgaaatcacc agtgtatttt tacaacaatt accaacaaca acaaacaaca aacaacatta
     1141 caattactat ttacaatttc tagaatggac aagcagaaga acggcatcaa ggccaacttc
     1201 aagatccgcc acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac
     1261 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     1321 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1381 gccgccggga tcactctcgg catggacgag ctgtacaagt acccatacga tgttccagat
     1441 tacgctgggc ccaggcctac tagtacaagt ttgtacaaaa aagctgaacg agaaacgtaa
     1501 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     1561 tgtaaaacac aacatatcca gtcactatga atcaactact tagatggtat tagtgacctg
     1621 tagtcgaccg acagccttcc aaatgttctt cgggtgatgc tgccaactta gtcgaccgac
     1681 agccttccaa atgttcttct caaacggaat cgtcgtatcc agcctactcg ctattgtcct
     1741 caatgccgta ttaaatcata aaaagaaata agaaaaagag gtgcgagcct cttttttgtg
     1801 tgacaaaata aaaacatcta cctattcata tacgctagtg tcatagtcct gaaaatcatc
     1861 tgcatcaaga acaatttcac aactcttata cttttctctt acaagtcgtt cggcttcatc
     1921 tggattttca gcctctatac ttactaaacg tgataaagtt tctgtaattt ctactgtatc
     1981 gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt
     2041 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
     2101 cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
     2161 gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg
     2221 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
     2281 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc
     2341 tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
     2401 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
     2461 accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
     2521 ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat
     2581 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
     2641 ttttagtaag ccggatccac gcggcgttta cgccccgccc tgccactcat cgcagtactg
     2701 ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat gatgaacctg
     2761 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     2821 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2881 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2941 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     3001 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     3061 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg
     3121 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     3181 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     3241 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     3301 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     3361 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     3421 gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg
     3481 gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt attcggcgca
     3541 aagtgcgtcg ggtgatgctg ccaacttagt cgactacagg tcactaatac catctaagta
     3601 gttgattcat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     3661 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     3721 gtacaaagtg gttctcgagg caaccaactt tagcctgctc aagcaagcag gagatgttga
     3781 ggaaaatcct ggccccccta agaaaaagag aaaggttccg aaaaagaaaa gaaaggtgcc
     3841 aaaaaaaaag agaaaggtaa tggtgagcaa gggcgaggag cttatcaaag agaatatgca
     3901 catgaagttg tatatggagg gtactgtgga taaccatcac tttaaatgta catcagaagg
     3961 agagggaaag ccttacgagg gtactcaaac aatgagaatt aaggttgtgg agggtggtcc
     4021 tcttccattt gctttcgata tcttggcaac atcttttctc tacggttcaa aaactttcat
     4081 taaccataca cagggtattc cagatttctt caagcagtct tttccagagg gattcacttg
     4141 ggaaagagtt actacttatg aggatggtgg tgttcttact gcaactcaag atacatctct
     4201 tcaggatgga tgcttgatct ataacgttaa gatcagagga gttaatttca cttcaaacgg
     4261 acctgttatg caaaagaaaa cattgggttg ggaggctttt actgagacat tgtacccagc
     4321 agatggaggt ttggaaggaa gaaacgatat ggctcttaaa ttggttggag gttctcatct
     4381 catcgcaaat gctaaaacta catatagatc aaagaaacct gctaagaacc tcaaaatgcc
     4441 aggagtttat tacgtggatt acagacttga aagaattaaa gaggcaaaca acgaaactta
     4501 tgttgaacag catgaggttg ctgtggcaag atactgtgat cttccttcta aactggggca
     4561 caaacttaat taagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
     4621 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     4681 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     4741 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     4801 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac
     4861 caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4921 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4981 actcacatta ggctgaatta ggcgcgccta tttctattaa ttcccgatct agtaacatag
     5041 atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt
     5101 attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc
     5161 attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg
     5221 caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatcgg
     5281 ggaaattcgg gggatcgatc ccgcgaactg tggacgagaa ctgtgccacc aagcgtaagg
     5341 ccgttctctc gcatttgcct tgctaggctc gcgcgagttg ctggctgagg cgttctcgaa
     5401 atcagctctt gttcggtcgg catctactct attcctttgc cctcggacga gtgctggggc
     5461 gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc
     5521 tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc
     5581 gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt
     5641 caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc
     5701 tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga
     5761 tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg
     5821 ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc
     5881 ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg
     5941 cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc
     6001 atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc
     6061 cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca
     6121 gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt gcacggcggg
     6181 agatgcaata ggtcaggctc tcgctgaatg ccccaatgtc aagcacttcc ggaatcggga
     6241 gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca tcggcgcagc
     6301 tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca cgagattctt
     6361 cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc agaaacttct
     6421 cgacagacgt cgcggtgagt tcaggctttt tcatatctta ttgccccccg ggatcagatc
     6481 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     6541 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     6601 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     6661 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     6721 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatctt gatcccctgc
     6781 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     6841 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     6901 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     6961 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     7021 tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc
     7081 gtgaagctga tccgtcgaga ttaatagaaa taaattcagc ctaattcggc gttaattcag
     7141 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     7201 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     7261 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     7321 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     7381 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     7441 ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     7501 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     7561 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     7621 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     7681 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg
     7741 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     7801 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     7861 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     7921 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     7981 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     8041 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     8101 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     8161 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     8221 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     8281 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     8341 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     8401 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     8461 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     8521 caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     8581 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     8641 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat
     8701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8881 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     8941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     9241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9421 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9481 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9541 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     9601 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     9661 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     9721 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     9781 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
     9841 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     9901 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     9961 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
    10021 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
    10081 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
    10141 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
    10201 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
    10261 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
    10321 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
    10381 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
    10441 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
    10501 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
    10561 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
    10621 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
    10681 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
    10741 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
    10801 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
    10861 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
    10921 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
    10981 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
    11041 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
    11101 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
    11161 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
    11221 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
    11281 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
    11341 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
    11401 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    11461 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    11521 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    11581 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    11641 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    11701 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    11761 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    11821 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    11881 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    11941 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    12001 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    12061 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    12121 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    12181 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    12241 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    12301 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    12361 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    12421 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    12481 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    12541 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    12601 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    12661 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    12721 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    12781 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    12841 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    12901 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    12961 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    13021 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    13081 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    13141 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    13201 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    13261 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    13321 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    13381 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    13441 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    13501 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    13561 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
    13621 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
