LOCUS       plasmid012_pLV-F       11494 bp DNA     circular SYN 31-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 31, 2024 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
COMMENT     [DEFINITION]: Contains Golden Gate cassette to express up to
FEATURES             Location/Qualifiers
     source          1..11494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..3677
                     /label=source [*] [5'] [5']
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..10409"
     source          4720..5435
                     /label=source [*] [5'] [3']
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..10409"
     source          5437..5441
                     /label=source [*] [3']
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..10409"
     source          5442..6161
                     /label=source [*] [5']
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..10409"
     source          6882..11494
                     /label=source [*] [3']
                     /note="organism=""synthetic DNA construct"" - 
                     mol_type=""other DNA"" - 1..10409"
     primer_bind     complement(47..66)
                     /label=pRS vectors, use to sequence yeast selectable _...
                     /note="label=pRS-marker - note=""pRS vectors, use to 
                     sequence yeast selectable  - marker"" - complement(47..66)"
     enhancer        238..617
                     /label=human cytomegalovirus immediate early enhancer_...
                     /note="label=CMV enhancer - note=""human cytomegalovirus 
                     immediate early enhancer"" - 238..617"
     promoter        619..817
                     /label=human cytomegalovirus (CMV) immediate early _CM...
                     /note="label=CMV promoter - note=""human cytomegalovirus 
                     (CMV) immediate early  - promoter"" - 619..817"
     primer_bind     772..792
                     /label=Human CMV immediate early promoter, forward pri...
                     /note="label=CMV-F - note=""Human CMV immediate early 
                     promoter, forward primer"" - 772..792"
     LTR             835..1015
                     /label=truncated 5' long terminal repeat (LTR) from HI...
                     /note="label=5' LTR (truncated) - note=""truncated 5' long 
                     terminal repeat (LTR) from HIV-1"" - 835..1015"
     misc_feature    1062..1187
                     /label=packaging signal of human immunodeficiency viru...
                     /note="label=HIV-1 Psi - note=""packaging signal of human 
                     immunodeficiency virus  - type 1"" - 1062..1187"
     misc_feature    1680..1913
                     /label=The Rev response element (RRE) of HIV-1 allows ...
                     /note="label=RRE - note=""The Rev response element (RRE) of
                     HIV-1 allows for  - Rev-dependent mRNA export from the 
                     nucleus to the  - cytoplasm."" - 1680..1913"
     misc_feature    2440..2557
                     /label=central polypurine tract and central terminatio...
                     /note="label=cPPT/CTS - note=""central polypurine tract and
                     central termination  - sequence of HIV-1"" - 2440..2557"
     protein_bind    2602..2635
                     /label=Cre-mediated recombination occurs in the 8-bp c...
                     /note="label=loxP - bound_moiety=""Cre recombinase"" - 
                     note=""Cre-mediated recombination occurs in the 8-bp core  
                     - sequence (GCATACAT)."" - 2602..2635"
     CDS             complement(2622..2948)
                     /label=lacZ-alpha
                     /note="codon_start=1 - gene=""lacZ fragment"" - 
                     product=""LacZ-alpha fragment of beta-galactosidase"" - 
                     label=lacZ-alpha - 
                     translation=""MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                      - 
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGATTVSY
                      - HNFV"" - complement("
     primer_bind     2798..2820
                     /label=In lacZ gene_M13/pUC Forward
                     /note="label=M13/pUC Forward - note=""In lacZ gene"" - 
                     2798..2820"
     primer_bind     2812..2829
                     /label=In lacZ gene. Also called M13-F20 or M13 (-21) ...
                     /note="label=M13 Forward - note=""In lacZ gene. Also called
                     M13-F20 or M13 (-21)  - Forward"" - 2812..2829"
     primer_bind     2813..2829
                     /label=common sequencing primer, one of multiple simil...
                     /note="label=M13 fwd - note=""common sequencing primer, one
                     of multiple similar  - variants"" - 2813..2829"
     primer_bind     2839..2858
                     /label=T7 promoter, forward primer_T7
                     /note="label=T7 - note=""T7 promoter, forward primer"" - 
                     2839..2858"
     promoter        2839..2857
                     /label=promoter for bacteriophage T7 RNA polymerase_T7...
                     /note="label=T7 promoter - note=""promoter for 
                     bacteriophage T7 RNA polymerase"" - 2839..2857"
     primer_bind     complement(2905..2925)
                     /label=T3 promoter, forward primer_T3
                     /note="label=T3 - note=""T3 promoter, forward primer"" - 
                     complement(2905..2925)"
     promoter        complement(2905..2923)
                     /label=promoter for bacteriophage T3 RNA polymerase_T3...
                     /note="label=T3 promoter - note=""promoter for 
                     bacteriophage T3 RNA polymerase"" - complement(2905..2923)"
     primer_bind     complement(2944..2960)
                     /label=common sequencing primer, one of multiple simil...
                     /note="label=M13 rev - note=""common sequencing primer, one
                     of multiple similar  - variants"" - complement(2944..2960)"
     protein_bind    2968..2984
                     /label=The lac repressor binds to the lac operator to ...
                     /note="label=lac operator - bound_moiety=""lac repressor 
                     encoded by lacI"" - note=""The lac repressor binds to the 
                     lac operator to  - inhibit transcription in E. coli. This 
                     inhibition can be  - relieved by adding lactose or  - 
                     isopropyl-beta-D-thiogalactopyranoside"
     promoter        complement(2992..3022)
                     /label=promoter for the E. coli lac operon_lac promoter
                     /note="label=lac promoter - note=""promoter for the E. coli
                     lac operon"" - complement(2992..3022)"
     protein_bind    3037..3058
                     /label=CAP binding activates transcription in the pres...
                     /note="label=CAP binding site - bound_moiety=""E. coli 
                     catabolite activator protein"" - note=""CAP binding 
                     activates transcription in the presence  - of cAMP."" - 
                     3037..3058"
     promoter        3135..3677
                     /label=human ubiquitin C promoter_UbC promoter [5']
                     /note="label=UbC promoter - note=""human ubiquitin C 
                     promoter"" - 3135..4346"
     promoter        4720..5388
                     /label=human ubiquitin C promoter_UbC promoter [3']
                     /note="label=UbC promoter - note=""human ubiquitin C 
                     promoter"" - 3135..4346"
     primer_bind     5244..5435
                     /label=Human Ubiquitin C (UbC) promoter, forward prime...
                     /note="label=hUBCpro-F - note=""Human Ubiquitin C (UbC) 
                     promoter, forward primer"" - 4183..4202"
     misc_feature    5435
                     /label=vertebrate consensus sequence for strong initia...
                     /note="regulatory_class=""other"" - note=""vertebrate 
                     consensus sequence for strong initiation  - of translation 
                     (Kozak, 1987)"" - 4393..4402"
     primer_bind     5437..6161
                     /label=Human Ubiquitin C (UbC) promoter, forward prime...
                     /note="label=hUBCpro-F - note=""Human Ubiquitin C (UbC) 
                     promoter, forward primer"" - 4183..4202"
     misc_feature    5437..5441
                     /label=vertebrate consensus sequence for strong initia...
                     /note="regulatory_class=""other"" - note=""vertebrate 
                     consensus sequence for strong initiation  - of translation 
                     (Kozak, 1987)"" - 4393..4402"
     CDS             5442..6161
                     /label=mammalian codon-optimized EGFP
                     /note="codon_start=1 - product=""enhanced GFP"" - 
                     label=EGFP - note=""mammalian codon-optimized"" - 
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                      - 
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                      - GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYN"
     misc_feature    5466..6054
                     /label=woodchuck hepatitis virus posttranscriptional _...
                     /note="label=WPRE - note=""woodchuck hepatitis virus 
                     posttranscriptional  - regulatory element"" - 5101..5689"
     primer_bind     complement(5519..5539)
                     /label=WPRE, reverse primer_WPRE-R
                     /note="label=WPRE-R - note=""WPRE, reverse primer"" - 
                     complement(5154..5174)"
     CDS             complement(5937..5948)
                     /label=Factor Xa site
                     /note="codon_start=1 - product=""Factor Xa recognition and 
                     cleavage site"" - label=Factor Xa site - 
                     translation=""IEGR"" - complement(5572..5583)"
     primer_bind     complement(6057..6073)
                     /label=common sequencing primer, one of multiple simil...
                     /note="label=KS primer - note=""common sequencing primer, 
                     one of multiple similar  - variants"" - 
                     complement(5692..5708)"
     primer_bind     complement(6058..6074)
                     /label=For pBluescript vector_pBluescriptKS
                     /note="label=pBluescriptKS - note=""For pBluescript 
                     vector"" - complement(5693..5709)"
     LTR             7299..7479
                     /label=truncated 5' long terminal repeat (LTR) from HI...
                     /note="label=5' LTR (truncated) - note=""truncated 5' long 
                     terminal repeat (LTR) from HIV-1"" - 6214..6394"
     primer_bind     complement(7505..7522)
                     /label=Bovine growth hormone terminator, reverse prime...
                     /note="label=BGH-rev - note=""Bovine growth hormone 
                     terminator, reverse primer.  - Also called BGH reverse"" - 
                     complement(6420..6437)"
     polyA_signal    7511..7735
                     /label=bovine growth hormone polyadenylation signal_bG...
                     /note="label=bGH poly(A) signal - note=""bovine growth 
                     hormone polyadenylation signal"" - 6426..6650"
     rep_origin      7781..8209
                     /direction=RIGHT
                     /label=f1 bacteriophage origin of replication; arrow _...
                     /note="direction=RIGHT - label=f1 ori - note=""f1 
                     bacteriophage origin of replication; arrow  - indicates 
                     direction of (+) strand synthesis"" - 6696..7124"
     primer_bind     complement(7868..7887)
                     /label=F1 origin, reverse primer_F1ori-R
                     /note="label=F1ori-R - note=""F1 origin, reverse primer"" -
                     complement(6783..6802)"
     primer_bind     8078..8099
                     /label=F1 origin, forward primer_F1ori-F
                     /note="label=F1ori-F - note=""F1 origin, forward primer"" -
                     6993..7014"
     primer_bind     complement(8218..8238)
                     /label=SV40 enhancer, reverse primer for pBABE vectors...
                     /note="label=pBABE 3' - note=""SV40 enhancer, reverse 
                     primer for pBABE vectors"" - complement(7133..7153)"
     promoter        8223..8552
                     /label=SV40 enhancer and early promoter_SV40 promoter
                     /note="label=SV40 promoter - note=""SV40 enhancer and early
                     promoter"" - 7138..7467"
     rep_origin      8403..8538
                     /direction=RIGHT
                     /label=SV40 origin of replication_SV40 ori
                     /note="label=SV40 ori - note=""SV40 origin of replication""
                     - 7318..7453"
     primer_bind     8465..8484
                     /label=SV40 promoter/origin, forward primer_SV40pro-F
                     /note="label=SV40pro-F - note=""SV40 promoter/origin, 
                     forward primer"" - 7380..7399"
     promoter        8600..8647
                     /label=synthetic bacterial promoter _EM7 promoter
                     /note="label=EM7 promoter - note=""synthetic bacterial 
                     promoter "" - 7515..7562"
     CDS             8666..9040
                     /label=confers resistance to bleomycin, phleomycin, an...
                     /note="codon_start=1 - gene=""Sh ble from 
                     Streptoalloteichus hindustanus"" - 
                     product=""antibiotic-binding protein"" - label=BleoR - 
                     note=""confers resistance to bleomycin, phleomycin, and  - 
                     Zeocin(TM)"" - 
                     translation=""MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                      - V"
     polyA_signal    9170..9291
                     /label=SV40 polyadenylation signal_SV40 poly(A) signal
                     /note="label=SV40 poly(A) signal - note=""SV40 
                     polyadenylation signal"" - 8085..8206"
     primer_bind     complement(9207..9226)
                     /label=SV40 polyA, reverse primer_SV40pA-R
                     /note="label=SV40pA-R - note=""SV40 polyA, reverse primer""
                     - complement(8122..8141)"
     primer_bind     9261..9280
                     /label=SV40 polyA terminator, reverse primer_EBV-rev
                     /note="label=EBV-rev - note=""SV40 polyA terminator, 
                     reverse primer"" - 8176..8195"
     primer_bind     complement(9340..9356)
                     /label=common sequencing primer, one of multiple simil...
                     /note="label=M13 rev - note=""common sequencing primer, one
                     of multiple similar  - variants"" - complement(8255..8271)"
     protein_bind    9364..9380
                     /label=The lac repressor binds to the lac operator to ...
                     /note="label=lac operator - bound_moiety=""lac repressor 
                     encoded by lacI"" - note=""The lac repressor binds to the 
                     lac operator to  - inhibit transcription in E. coli. This 
                     inhibition can be  - relieved by adding lactose or  - 
                     isopropyl-beta-D-thiogalactopyranoside"
     promoter        complement(9388..9418)
                     /label=promoter for the E. coli lac operon_lac promoter
                     /note="label=lac promoter - note=""promoter for the E. coli
                     lac operon"" - complement(8303..8333)"
     protein_bind    9433..9454
                     /label=CAP binding activates transcription in the pres...
                     /note="label=CAP binding site - bound_moiety=""E. coli 
                     catabolite activator protein"" - note=""CAP binding 
                     activates transcription in the presence  - of cAMP."" - 
                     8348..8369"
     primer_bind     complement(9571..9588)
                     /label=L4440 vector, forward primer_L4440
                     /note="label=L4440 - note=""L4440 vector, forward primer"" 
                     - complement(8486..8503)"
     rep_origin      complement(9742..10330)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o...
                     /note="direction=LEFT - label=ori - note=""high-copy-number
                     ColE1/pMB1/pBR322/pUC origin of  - replication"" - 
                     complement(8657..9245)"
     primer_bind     complement(9822..9841)
                     /label=pBR322 origin, forward primer_pBR322ori-F
                     /note="label=pBR322ori-F - note=""pBR322 origin, forward 
                     primer"" - complement(8737..8756)"
     CDS             complement(10501..11361)
                     /label=confers resistance to ampicillin, carbenicillin...
                     /note="codon_start=1 - gene=""bla"" - 
                     product=""beta-lactamase"" - label=AmpR - note=""confers 
                     resistance to ampicillin, carbenicillin, and - related 
                     antibiotics"" - 
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                      - ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQ"
     primer_bind     11124..11143
                     /label=Ampicillin resistance gene, reverse primer_Amp-R
                     /note="label=Amp-R - note=""Ampicillin resistance gene, 
                     reverse primer"" - 10039..10058"
     promoter        complement(11362..11466)
                     /label=AmpR promoter
                     /note="gene=""bla"" - label=AmpR promoter - 
                     complement(10277..10381)"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aataacttcg tatagcatac attatacgaa gttatgatag
     2641 atccgacgcc gccatctcta ggcccgcgcc ggccccctcg cacagacttg tgggagaagc
     2701 tcggctactc ccctgccccg gttaatttgc atataatatt tcctagtaac tatagaggct
     2761 taatgtgcga taaaagacag ataatctgtt ctttttaata ctagctacat tttacatgat
     2821 aggcttggat ttctataaga gatacaaata ctaaattatt attttaaaaa acagcacaaa
     2881 aggaaactca ccctaactgt aaagtaattg tgtgttttga gactataaat atcccttgga
     2941 gaaaagcctt gtttgagcgc tctggatctc tcaggtttta gagctagaaa tagcaagtta
     3001 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttgta
     3061 cgctcagcgg agaaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta
     3121 gccttatttt aacttgctat ttctagctct aaaaccggga agtgagcggg ctgccggtgt
     3181 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     3241 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     3301 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattccaa
     3361 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
     3421 cctcttcctg cccgaccttg gatcgatccg cctgcctgaa cgctgacgtc atcaacccgc
     3481 tccaaggaat cgcgggccca gtgtcactag gcgggaacac ccagcgcgcg tgcgccctgg
     3541 caggaagatg gctgtgaggg acaggggagt ggcgccctgc aatatttgca tgtcgctatg
     3601 tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct
     3661 gtatgagacc actctttccc aagtagctgc tccagtcgga gttttagagc tagaaatagc
     3721 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     3781 tttggtcatc agtccgagaa aaaaagcacc gactcggtgc cactttttca agttgataac
     3841 ggactagcct tattttaact tgctatttct agctctaaaa cgaggtaagc gctcgtcttt
     3901 cgaggtaccc aagcggcgca caagctatat aaacctgaag gaagtctcaa ctttacactt
     3961 aggtcaagtt gcttatcgta ctagagcttc agcaggaaat ttaactaaaa tctaatttaa
     4021 ccagcatagc aaatatcatt tattcccaaa atgctaaagt ttgagataaa cggacttgat
     4081 ttccggctgt tttgacacta tccagaatgc cttgcagatg ggtggggcat gctaaatact
     4141 gcagacaatt aattaacccg tgtcggctcc agatctggcc tccgcgccgg gttttggcgc
     4201 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     4261 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     4321 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     4381 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     4441 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     4501 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     4561 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     4621 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     4681 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     4741 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     4801 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     4861 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     4921 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     4981 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     5041 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     5101 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     5161 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     5221 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     5281 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     5341 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttgggct
     5401 gcaggtcgac tctagaggat ccccgggtac cggtcgccac catggtgagc aagggcgagg
     5461 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     5521 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     5581 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     5641 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     5701 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     5761 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5821 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5881 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     5941 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     6001 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     6061 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     6121 ccgccgggat cactctcggc atggacgagc tgtacaagta agaattcgat atcaagctta
     6181 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6241 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6301 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6361 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6421 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6481 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6541 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6601 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6661 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6721 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     6781 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6841 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6901 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6961 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     7021 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     7081 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     7141 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     7201 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     7261 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     7321 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7381 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7441 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga
     7501 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7561 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7621 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     7681 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     7741 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     7801 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7861 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7921 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7981 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8041 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8101 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8161 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     8221 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     8281 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     8341 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     8401 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8461 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     8521 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     8581 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     8641 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     8701 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     8761 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     8821 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     8881 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     8941 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     9001 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     9061 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     9121 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     9181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9301 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     9361 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     9421 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     9481 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     9541 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9601 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9661 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9721 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9781 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9841 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9901 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9961 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    10021 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10081 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10141 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
    10201 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10261 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10321 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10381 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10441 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10501 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    10561 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    10621 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    10681 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    10741 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    10801 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    10861 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    10921 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    10981 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    11041 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    11101 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
    11161 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    11221 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    11281 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    11341 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    11401 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    11461 tccgcgcaca tttccccgaa aagtgccacc tgac
//
