LOCUS       Exported                8271 bp ds-DNA     circular SYN 02-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 
            pHAGE2-EF1alpha-Sox2.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          3530..4609
                     /organism="Homo sapiens"
                     /chromosome="6"
                     /map="6p21.33"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
     LTR             1..636
                     /label=5' LTR
                     /label=5'\LTR
                     /note="gene: LTR vntifkey: 19 note: Long Terminal Repeat 
                     locus_tag: 5'\LTR label: 5'\LTR ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1..454
                     /label=HIV U3
                     /label=HIV\U3
                     /note="vntifkey: 21 note: Full Length HIV U3 locus_tag: 
                     HIV\U3 label: HIV\U3 ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(158..179)
                     /label=pHAGE-A0050-8050
                     /note="vntifkey: 28 locus_tag: pHAGE-A0050-8050 label: 
                     pHAGE-A0050-8050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     401..421
                     /label=pHAGE-S501-1000
                     /note="vntifkey: 28 locus_tag: pHAGE-S501-1000 label: 
                     pHAGE-S501-1000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    455..551
                     /label=HIV R
                     /label=HIV\R
                     /note="vntifkey: 21 note: HIV Repeat Region locus_tag: 
                     HIV\R label: HIV\R ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     polyA_site      550
                     /label=pA Site
                     /label=pA\Site
                     /note="vntifkey: 26 note: Exact base to which Poly-A is 
                     added locus_tag: pA\Site label: pA\Site ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     5'UTR           551..635
                     /label=5'\UTR
                     /note="vntifkey: 52 note: HIV 5' UTR locus_tag: 5'\UTR 
                     label: 5'\UTR ApEinfo_revcolor: pink ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    552..636
                     /label=HIV U5
                     /label=HIV\U5
                     /note="vntifkey: 21 note: HIV U5 locus_tag: HIV\U5 label: 
                     HIV\U5 ApEinfo_revcolor: #000000 ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     polyA_signal    570..577
                     /label=G-T cluster
                     /label=G-T\cluster
                     /note="vntifkey: 25 note: GT Cluster shown to be necessary 
                     for Poly-A siteactivity by Cullen in J Virol. 1989 
                     January;63(1):421-424 locus_tag: G-T\cluster label: 
                     G-T\cluster ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    636..658
                     /label=PBS-K
                     /note="vntifkey: 21 note: tRNA binding site for Lysine tRNA
                     locus_tag: PBS-K label: PBS-K ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    637..1155
                     /label=PSI
                     /note="gene: psi product: pbs-gag vntifkey: 21 note: PSI 
                     Packaging Sequence locus_tag: PSI label: PSI 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     complement(667..690)
                     /label=pHAGE-A0550-0050
                     /note="vntifkey: 28 locus_tag: pHAGE-A0550-0050 label: 
                     pHAGE-A0550-0050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    697..806
                     /label=HIV-PSI
                     /note="vntifkey: 21 locus_tag: HIV-PSI label: HIV-PSI 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     stem_loop       697..731
                     /label=SL-1
                     /note="vntifkey: 39 note: PSI Stem loop 1 locus_tag: SL-1 
                     label: SL-1 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     stem_loop       736..754
                     /label=SL-2
                     /note="vntifkey: 39 note: PSI Stem loop 2 locus_tag: SL-2 
                     label: SL-2 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    744..745
                     /label=5' SD 289
                     /label=5'\SD\289
                     /note="vntifkey: 38 note: Major 5' Splice Donor 289 
                     locus_tag: 5'\SD\289 label: 5'\SD\289 ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     stem_loop       766..779
                     /label=SL-3
                     /note="vntifkey: 39 note: PSI Stem Loop 3 locus_tag: SL-3 
                     label: SL-3 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             790..1144
                     /label=HIV gag
                     /label=HIV\gag
                     /note="vntifkey: 4 note: HIV gag sequence from 336bp to 
                     688bp (Clone toPvuII site in HIV and filled in ClaI digest 
                     to take gagCDS out of frame) locus_tag: HIV\gag label: 
                     HIV\gag ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             790..1144
                     /label=HIV gag
                     /label=HIV\gag
                     /note="vntifkey: 4 note: HIV gag sequence from 336bp to 
                     688bp (Clone toPvuII site in HIV and filled in ClaI digest 
                     to take gagCDS out of frame) locus_tag: HIV\gag label: 
                     HIV\gag ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     stem_loop       793..806
                     /label=SL-4
                     /note="vntifkey: 39 note: PSI Stem Loop 4 locus_tag: SL-4 
                     label: SL-4 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    807..1144
                     /label=HR-ePSI
                     /note="vntifkey: 21 note: HIV gag sequence 351bp to 688bp 
                     locus_tag: HR-ePSI label: HR-ePSI ApEinfo_revcolor: #000000
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    833..834
                     /label=Fill-in
                     /note="vntifkey: 62 note: Filled in Cla Site from original 
                     HIV genome todisrupt gag polyprotein ORF locus_tag: Fill-in
                     label: Fill-in ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    853..855
                     /label=Stop Codon
                     /label=Stop\Codon
                     /note="vntifkey: 21 note: Stop codon in gag put in frame by
                     ClaI fill in locus_tag: Stop\Codon label: Stop\Codon 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     898..919
                     /label=pHAGE-S1001-1500
                     /note="vntifkey: 28 locus_tag: pHAGE-S1001-1500 label: 
                     pHAGE-S1001-1500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             1156..2013
                     /label=env
                     /note="vntifkey: 4 note: HIV env sequence from bp7168 to 
                     8025 (contains RRE)Cloned from BglII to BamHI out of HIV 
                     locus_tag: env label: env ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             1156..2013
                     /label=env
                     /note="vntifkey: 4 note: HIV env sequence from bp7168 to 
                     8025 (contains RRE)Cloned from BglII to BamHI out of HIV 
                     locus_tag: env label: env ApEinfo_fwdcolor: pink 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     complement(1166..1189)
                     /label=pHAGE-A1050-0550
                     /note="vntifkey: 28 locus_tag: pHAGE-A1050-0550 label: 
                     pHAGE-A1050-0550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1245..1593
                     /label=RRE
                     /note="vntifkey: 21 note: Rev Responsive Element according 
                     to Belasco inMolecular Cell, Vol. 7, 603-614, March, 2001 
                     locus_tag: RRE(1) label: RRE(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1303..1536
                     /label=RRE
                     /note="gene: rre product: minimal RRE vntifkey: 21 note: 
                     Rev Responsive Element locus_tag: RRE label: RRE 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1357
                     /label=RRE Mutation
                     /label=RRE\Mutation
                     /note="vntifkey: 62 note: This G is a C in the WT HIV-1 
                     genome locus_tag: RRE\Mutation label: RRE\Mutation 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     1391..1417
                     /label=pHAGE-S1501-2000
                     /note="vntifkey: 28 locus_tag: pHAGE-S1501-2000 label: 
                     pHAGE-S1501-2000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(1650..1678)
                     /label=pHAGE-A1550-1050
                     /note="vntifkey: 28 locus_tag: pHAGE-A1550-1050 label: 
                     pHAGE-A1550-1050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     1898..1922
                     /label=pHAGE-S2001-2500
                     /note="vntifkey: 28 locus_tag: pHAGE-S2001-2500 label: 
                     pHAGE-S2001-2500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1911..1912
                     /label=SA 7925
                     /label=SA\7925
                     /note="vntifkey: 38 locus_tag: SA\7925 label: SA\7925 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2014..2027
                     /label=Junk
                     /note="vntifkey: 21 note: Linker Junk Sequence probably 
                     left over fromcloning locus_tag: Junk label: Junk 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2034..2211
                     /label=HIV cpPu (Trip)
                     /label=HIV\cpPu\(Trip)
                     /note="gene: cppt vntifkey: 21 note: Central Poly Purine 
                     Track locus_tag: HIV\cpPu\(Trip) label: HIV\cpPu\(Trip) 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2062..2076
                     /label=cPPT
                     /note="locus_tag: cPPT label: cPPT ApEinfo_revcolor: green 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(2084..2112)
                     /label=pHAGE-A2050-1550
                     /note="vntifkey: 28 locus_tag: pHAGE-A2050-1550 label: 
                     pHAGE-A2050-1550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    2188..2189
                     /label=SA 4459
                     /label=SA\4459
                     /note="vntifkey: 38 locus_tag: SA\4459 label: SA\4459 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2218..2614
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter label: EF1a promoter 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2367..2445
                     /label=EF-1 promoter
                     /note="locus_tag: EF-1 promoter label: EF-1 promoter 
                     ApEinfo_revcolor: #bab7be ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2616..3456
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter label: EF1a promoter 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3457..3485
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter(1) label: EF1a promoter(1) 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             3530..4609
                     /codon_start=1
                     /gene="POU5F1"
                     /gene_synonym="Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4"
                     /product="POU domain, class 5, transcription factor 1 
                     isoform 1"
                     /label=human OCT4/ POU5F1
                     /note="isoform 1 is encoded by transcript variant 1; 
                     POU-type homeodomain-containing DNA-binding protein; POU 
                     domain transcription factor OCT4; POU domain, class 5, 
                     transcription factor 1; octamer-binding protein 3; 
                     octamer-binding protein 4; octamer-binding transcription 
                     factor 3; POU class 5 homeobox 1 transcript variant OCT4B1;
                     POU class 5 homeobox 1 transcript variant OCT4B3; POU class
                     5 homeobox 1 transcript variant OCT4B4; POU class 5 
                     homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 
                     1 transcript variant OCT4B6; POU class 5 homeobox 1 
                     transcript variant OCT4B5; POU classV homeobox 1 variant 2"
                     /db_xref="CCDS:CCDS34391.1"
                     /db_xref="GeneID:5460"
                     /db_xref="HGNC:HGNC:9221"
                     /db_xref="MIM:164177"
                     /protein_id="NP_002692.2"
                     /translation="MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGP
                     GIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVE
                     SNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYT
                     QADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICK
                     AETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNR
                     RQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFP
                     EGEAFPPVSVTTLGSPMHSN"
     mat_peptide     4628..4693
                     /locus_tag="3xFLAG"
                     /label=3xFLAG
     misc_feature    4712..5303
                     /label=WPRE
                     /note="gene: WPRE vntifkey: 21 note: Woodchuck Hepatitis 
                     Virus PosttranscriptionalRegulatory Element locus_tag: WPRE
                     label: WPRE ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(4740..4774)
                     /label=pHAGE 3' CDS
                     /label=pHAGE\3'\CDS
                     /note="vntifkey: 28 locus_tag: pHAGE\3'\CDS label: 
                     pHAGE\3'\CDS ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     complement(4818..4841)
                     /label=pHAGE-A3550-3050
                     /note="vntifkey: 28 locus_tag: pHAGE-A3550-3050 label: 
                     pHAGE-A3550-3050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     4971..4991
                     /label=pHAGE-S4001-4500
                     /note="vntifkey: 28 locus_tag: pHAGE-S4001-4500 label: 
                     pHAGE-S4001-4500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5016..5095
                     /label=WPRE
                     /note="locus_tag: WPRE(1) label: WPRE(1) ApEinfo_revcolor: 
                     green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     LTR             5308..5629
                     /label=3' LTR
                     /label=3'\LTR
                     /note="vntifkey: 19 locus_tag: 3'\LTR label: 3'\LTR 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5308..5409
                     /label=HIV nef
                     /label=HIV\nef
                     /note="vntifkey: 21 note: HIV nef locus_tag: HIV\nef label:
                     HIV\nef ApEinfo_revcolor: #000000 ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5358..5372
                     /label=HIV PPT
                     /label=HIV\PPT
                     /note="vntifkey: 21 note: PolyPurine Tract locus_tag: 
                     HIV\PPT label: HIV\PPT ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5358..5372
                     /label=cPPT
                     /note="locus_tag: cPPT(1) label: cPPT(1) ApEinfo_revcolor: 
                     green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5375..5411
                     /label=dU3
                     /note="vntifkey: 21 note: 1st Half of deleted U3 locus_tag:
                     dU3 label: dU3 ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5431..5449
                     /label=dU3
                     /note="vntifkey: 21 note: 2nd Half of deleted U3 locus_tag:
                     dU3(1) label: dU3(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5450..5546
                     /label=HIV R
                     /label=HIV\R
                     /note="vntifkey: 21 locus_tag: HIV\R(1) label: HIV\R(1) 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5547..5629
                     /label=HIV U5
                     /label=HIV\U5
                     /note="vntifkey: 21 note: HIV U5 locus_tag: HIV\U5(1) 
                     label: HIV\U5(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     5661..5683
                     /label=pHAGE-S6001-6500
                     /note="vntifkey: 28 locus_tag: pHAGE-S6001-6500 label: 
                     pHAGE-S6001-6500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     promoter        5832..5866
                     /label=P-Bla
                     /note="vntifkey: 30 note: Beta Lactamase Promoter 
                     locus_tag: P-Bla label: P-Bla ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             5885..6724
                     /label=AMPr
                     /note="gene: AMPr product: beta-lactamase (mature form) 
                     vntifkey: 4 note: Ampicillin Resistance Gene locus_tag: 
                     AMPr label: AMPr ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             5885..6724
                     /label=AMPr
                     /note="gene: AMPr product: beta-lactamase (mature form) 
                     vntifkey: 4 note: Ampicillin Resistance Gene locus_tag: 
                     AMPr label: AMPr ApEinfo_fwdcolor: pink ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(5934..5957)
                     /label=pHAGE-A6050-5550
                     /note="vntifkey: 28 locus_tag: pHAGE-A6050-5550 label: 
                     pHAGE-A6050-5550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             6065..6724
                     /label=AmpR
                     /note="locus_tag: AmpR label: AmpR ApEinfo_revcolor: yellow
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             6065..6724
                     /label=AmpR
                     /note="locus_tag: AmpR label: AmpR ApEinfo_fwdcolor: yellow
                     ApEinfo_revcolor: yellow ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     6134..6158
                     /label=pHAGE-S6501-7000
                     /note="vntifkey: 28 locus_tag: pHAGE-S6501-7000 label: 
                     pHAGE-S6501-7000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(6403..6426)
                     /label=pHAGE-A6550-6050
                     /note="vntifkey: 28 locus_tag: pHAGE-A6550-6050 label: 
                     pHAGE-A6550-6050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     6654..6678
                     /label=pHAGE-S7001-7500
                     /note="vntifkey: 28 locus_tag: pHAGE-S7001-7500 label: 
                     pHAGE-S7001-7500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     rep_origin      6822..7504
                     /label=ColE1 origin
                     /note="locus_tag: ColE1 origin label: ColE1 origin 
                     ApEinfo_fwdcolor: gray50 ApEinfo_revcolor: gray50 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(6914..6935)
                     /label=pHAGE-A7050-6550
                     /note="vntifkey: 28 locus_tag: pHAGE-A7050-6550 label: 
                     pHAGE-A7050-6550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     rep_origin      6953..7486
                     /label=ORI
                     /note="gene: ORI product: ColE1 origin of replication 
                     vntifkey: 33 note: Bacterial Origin of Replication 
                     locus_tag: ORI label: ORI ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     7158..7182
                     /label=pHAGE-S7501-8000
                     /note="vntifkey: 28 locus_tag: pHAGE-S7501-8000 label: 
                     pHAGE-S7501-8000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(7425..7446)
                     /label=pHAGE-A7550-7050
                     /note="vntifkey: 28 locus_tag: pHAGE-A7550-7050 label: 
                     pHAGE-A7550-7050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     7661..7680
                     /label=pHAGE-S8001-8500
                     /note="vntifkey: 28 locus_tag: pHAGE-S8001-8500 label: 
                     pHAGE-S8001-8500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    7723..7846
                     /label=SV40 ori
                     /label=SV40\ori
                     /note="gene: SV40 ori vntifkey: 21 locus_tag: SV40\ori 
                     label: SV40\ori ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(7901..7927)
                     /label=pHAGE-A8050-7550
                     /note="vntifkey: 28 locus_tag: pHAGE-A8050-7550 label: 
                     pHAGE-A8050-7550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_binding    7967..7989
                     /label=LacO
                     /note="locus_tag: LacO label: LacO ApEinfo_revcolor: 
                     #6495ed ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} 
                     {} 0}width 5 offset 0"
     primer_bind     8166..8194
                     /label=pHAGE-S1-500
                     /note="vntifkey: 28 locus_tag: pHAGE-S1-500 label: 
                     pHAGE-S1-500 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgat
     2221 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     2281 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     2341 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2401 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2461 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2521 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2581 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2641 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2701 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2761 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     2821 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     2881 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     2941 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3001 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3061 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3121 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3181 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3241 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3301 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3361 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3421 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3481 cgtgagcggc caagggcgaa ttctgcagat atccatcaca ctggctagca tggcgggaca
     3541 cctggcttcg gatttcgcct tctcgccccc tccaggtggt ggaggtgatg ggccaggggg
     3601 gccggagccg ggctgggttg atcctcggac ctggctaagc ttccaaggcc ctcctggagg
     3661 gccaggaatc gggccggggg ttgggccagg ctctgaggtg tgggggattc ccccatgccc
     3721 cccgccgtat gagttctgtg gggggatggc gtactgtggg ccccaggttg gagtggggct
     3781 agtgccccaa ggcggcttgg agacctctca gcctgagggc gaagcaggag tcggggtgga
     3841 gagcaactcc gatggggcct ccccggagcc ctgcaccgtc acccctggtg ccgtgaagct
     3901 ggagaaggag aagctggagc aaaacccgga ggagtcccag gacatcaaag ctctgcagaa
     3961 agaactcgag caatttgcca agctcctgaa gcagaagagg atcaccctgg gatatacaca
     4021 ggccgatgtg gggctcaccc tgggggttct atttgggaag gtattcagcc aaacgaccat
     4081 ctgccgcttt gaggctctgc agcttagctt caagaacatg tgtaagctgc ggcccttgct
     4141 gcagaagtgg gtggaggaag ctgacaacaa tgaaaatctt caggagatat gcaaagcaga
     4201 aaccctcgtg caggcccgaa agagaaagcg aaccagtatc gagaaccgag tgagaggcaa
     4261 cctggagaat ttgttcctgc agtgcccgaa acccacactg cagcagatca gccacatcgc
     4321 ccagcagctt gggctcgaga aggatgtggt ccgagtgtgg ttctgtaacc ggcgccagaa
     4381 gggcaagcga tcaagcagcg actatgcaca acgagaggat tttgaggctg ctgggtctcc
     4441 tttctcaggg ggaccagtgt cctttcctct ggccccaggg ccccattttg gtaccccagg
     4501 ctatgggagc cctcacttca ctgcactgta ctcctcggtc cctttccctg agggggaagc
     4561 ctttccccct gtctccgtca ccactctggg ctctcccatg cattcaaaca ttacgcgtgg
     4621 cagcggcgac tacaaggacc acgacggcga ctacaaggac cacgacatcg actacaagga
     4681 cgacgacgac aagtagtaaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg
     4741 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4801 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4861 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4921 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4981 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     5041 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     5101 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     5161 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     5221 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     5281 ccctttgggc cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta
     5341 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5401 agacaagatc acctgcagga caggcgcgcc ctgctttttg cttgtactgg gtctctctgg
     5461 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5521 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5581 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccgggcgatt
     5641 aaggaaaggg ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     5701 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5761 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg
     6121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6721 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6781 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6841 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6901 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6961 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7021 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7081 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7141 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7201 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7261 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7321 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7381 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7441 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7501 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7561 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7621 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7681 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcaagctca tggctgacta
     7741 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     7801 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt
     7861 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7921 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7981 ataacaattt cacacaggaa acagctatga catgattacg aatttcacaa ataaagcatt
     8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8101 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     8161 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     8221 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag t
//
