LOCUS       Exported                8142 bp ds-DNA     circular SYN 02-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 
            pHAGE2-EF1alpha-Sox2.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..636
                     /label=5' LTR
                     /label=5'\LTR
                     /note="gene: LTR vntifkey: 19 note: Long Terminal Repeat 
                     locus_tag: 5'\LTR label: 5'\LTR ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1..454
                     /label=HIV U3
                     /label=HIV\U3
                     /note="vntifkey: 21 note: Full Length HIV U3 locus_tag: 
                     HIV\U3 label: HIV\U3 ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(158..179)
                     /label=pHAGE-A0050-8050
                     /note="vntifkey: 28 locus_tag: pHAGE-A0050-8050 label: 
                     pHAGE-A0050-8050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     401..421
                     /label=pHAGE-S501-1000
                     /note="vntifkey: 28 locus_tag: pHAGE-S501-1000 label: 
                     pHAGE-S501-1000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    455..551
                     /label=HIV R
                     /label=HIV\R
                     /note="vntifkey: 21 note: HIV Repeat Region locus_tag: 
                     HIV\R label: HIV\R ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     polyA_site      550
                     /label=pA Site
                     /label=pA\Site
                     /note="vntifkey: 26 note: Exact base to which Poly-A is 
                     added locus_tag: pA\Site label: pA\Site ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     5'UTR           551..635
                     /label=5'\UTR
                     /note="vntifkey: 52 note: HIV 5' UTR locus_tag: 5'\UTR 
                     label: 5'\UTR ApEinfo_revcolor: pink ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    552..636
                     /label=HIV U5
                     /label=HIV\U5
                     /note="vntifkey: 21 note: HIV U5 locus_tag: HIV\U5 label: 
                     HIV\U5 ApEinfo_revcolor: #000000 ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     polyA_signal    570..577
                     /label=G-T cluster
                     /label=G-T\cluster
                     /note="vntifkey: 25 note: GT Cluster shown to be necessary 
                     for Poly-A siteactivity by Cullen in J Virol. 1989 
                     January;63(1):421-424 locus_tag: G-T\cluster label: 
                     G-T\cluster ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    636..658
                     /label=PBS-K
                     /note="vntifkey: 21 note: tRNA binding site for Lysine tRNA
                     locus_tag: PBS-K label: PBS-K ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    637..1155
                     /label=PSI
                     /note="gene: psi product: pbs-gag vntifkey: 21 note: PSI 
                     Packaging Sequence locus_tag: PSI label: PSI 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     complement(667..690)
                     /label=pHAGE-A0550-0050
                     /note="vntifkey: 28 locus_tag: pHAGE-A0550-0050 label: 
                     pHAGE-A0550-0050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    697..806
                     /label=HIV-PSI
                     /note="vntifkey: 21 locus_tag: HIV-PSI label: HIV-PSI 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     stem_loop       697..731
                     /label=SL-1
                     /note="vntifkey: 39 note: PSI Stem loop 1 locus_tag: SL-1 
                     label: SL-1 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     stem_loop       736..754
                     /label=SL-2
                     /note="vntifkey: 39 note: PSI Stem loop 2 locus_tag: SL-2 
                     label: SL-2 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    744..745
                     /label=5' SD 289
                     /label=5'\SD\289
                     /note="vntifkey: 38 note: Major 5' Splice Donor 289 
                     locus_tag: 5'\SD\289 label: 5'\SD\289 ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     stem_loop       766..779
                     /label=SL-3
                     /note="vntifkey: 39 note: PSI Stem Loop 3 locus_tag: SL-3 
                     label: SL-3 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             790..1144
                     /label=HIV gag
                     /label=HIV\gag
                     /note="vntifkey: 4 note: HIV gag sequence from 336bp to 
                     688bp (Clone toPvuII site in HIV and filled in ClaI digest 
                     to take gagCDS out of frame) locus_tag: HIV\gag label: 
                     HIV\gag ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             790..1144
                     /label=HIV gag
                     /label=HIV\gag
                     /note="vntifkey: 4 note: HIV gag sequence from 336bp to 
                     688bp (Clone toPvuII site in HIV and filled in ClaI digest 
                     to take gagCDS out of frame) locus_tag: HIV\gag label: 
                     HIV\gag ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     stem_loop       793..806
                     /label=SL-4
                     /note="vntifkey: 39 note: PSI Stem Loop 4 locus_tag: SL-4 
                     label: SL-4 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    807..1144
                     /label=HR-ePSI
                     /note="vntifkey: 21 note: HIV gag sequence 351bp to 688bp 
                     locus_tag: HR-ePSI label: HR-ePSI ApEinfo_revcolor: #000000
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    833..834
                     /label=Fill-in
                     /note="vntifkey: 62 note: Filled in Cla Site from original 
                     HIV genome todisrupt gag polyprotein ORF locus_tag: Fill-in
                     label: Fill-in ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    853..855
                     /label=Stop Codon
                     /label=Stop\Codon
                     /note="vntifkey: 21 note: Stop codon in gag put in frame by
                     ClaI fill in locus_tag: Stop\Codon label: Stop\Codon 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     898..919
                     /label=pHAGE-S1001-1500
                     /note="vntifkey: 28 locus_tag: pHAGE-S1001-1500 label: 
                     pHAGE-S1001-1500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             1156..2013
                     /label=env
                     /note="vntifkey: 4 note: HIV env sequence from bp7168 to 
                     8025 (contains RRE)Cloned from BglII to BamHI out of HIV 
                     locus_tag: env label: env ApEinfo_fwdcolor: pink 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             1156..2013
                     /label=env
                     /note="vntifkey: 4 note: HIV env sequence from bp7168 to 
                     8025 (contains RRE)Cloned from BglII to BamHI out of HIV 
                     locus_tag: env label: env ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(1166..1189)
                     /label=pHAGE-A1050-0550
                     /note="vntifkey: 28 locus_tag: pHAGE-A1050-0550 label: 
                     pHAGE-A1050-0550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1245..1593
                     /label=RRE
                     /note="vntifkey: 21 note: Rev Responsive Element according 
                     to Belasco inMolecular Cell, Vol. 7, 603-614, March, 2001 
                     locus_tag: RRE(1) label: RRE(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1303..1536
                     /label=RRE
                     /note="gene: rre product: minimal RRE vntifkey: 21 note: 
                     Rev Responsive Element locus_tag: RRE label: RRE 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1357
                     /label=RRE Mutation
                     /label=RRE\Mutation
                     /note="vntifkey: 62 note: This G is a C in the WT HIV-1 
                     genome locus_tag: RRE\Mutation label: RRE\Mutation 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     1391..1417
                     /label=pHAGE-S1501-2000
                     /note="vntifkey: 28 locus_tag: pHAGE-S1501-2000 label: 
                     pHAGE-S1501-2000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(1650..1678)
                     /label=pHAGE-A1550-1050
                     /note="vntifkey: 28 locus_tag: pHAGE-A1550-1050 label: 
                     pHAGE-A1550-1050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     1898..1922
                     /label=pHAGE-S2001-2500
                     /note="vntifkey: 28 locus_tag: pHAGE-S2001-2500 label: 
                     pHAGE-S2001-2500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1911..1912
                     /label=SA 7925
                     /label=SA\7925
                     /note="vntifkey: 38 locus_tag: SA\7925 label: SA\7925 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2014..2027
                     /label=Junk
                     /note="vntifkey: 21 note: Linker Junk Sequence probably 
                     left over fromcloning locus_tag: Junk label: Junk 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2034..2211
                     /label=HIV cpPu (Trip)
                     /label=HIV\cpPu\(Trip)
                     /note="gene: cppt vntifkey: 21 note: Central Poly Purine 
                     Track locus_tag: HIV\cpPu\(Trip) label: HIV\cpPu\(Trip) 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2062..2076
                     /label=cPPT
                     /note="locus_tag: cPPT label: cPPT ApEinfo_revcolor: green 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(2084..2112)
                     /label=pHAGE-A2050-1550
                     /note="vntifkey: 28 locus_tag: pHAGE-A2050-1550 label: 
                     pHAGE-A2050-1550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    2188..2189
                     /label=SA 4459
                     /label=SA\4459
                     /note="vntifkey: 38 locus_tag: SA\4459 label: SA\4459 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2218..2614
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter label: EF1a promoter 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2367..2445
                     /label=EF-1 promoter
                     /note="locus_tag: EF-1 promoter label: EF-1 promoter 
                     ApEinfo_revcolor: #bab7be ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2616..3456
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter label: EF1a promoter 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3457..3485
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter(1) label: EF1a promoter(1) 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             3530..4480
                     /codon_start=1
                     /label=human SOX2
                     /translation="MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPM
                     NAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKE
                     HPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHM
                     NGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPT
                     YSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGA
                     EVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM"
     misc_feature    3650..3856
                     /label=HMG box
     mat_peptide     4499..4564
                     /locus_tag="3xFLAG"
                     /label=3xFLAG
     misc_feature    4583..5174
                     /label=WPRE
                     /note="gene: WPRE vntifkey: 21 note: Woodchuck Hepatitis 
                     Virus PosttranscriptionalRegulatory Element locus_tag: WPRE
                     label: WPRE ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(4611..4645)
                     /label=pHAGE 3' CDS
                     /label=pHAGE\3'\CDS
                     /note="vntifkey: 28 locus_tag: pHAGE\3'\CDS label: 
                     pHAGE\3'\CDS ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     complement(4689..4712)
                     /label=pHAGE-A3550-3050
                     /note="vntifkey: 28 locus_tag: pHAGE-A3550-3050 label: 
                     pHAGE-A3550-3050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     4842..4862
                     /label=pHAGE-S4001-4500
                     /note="vntifkey: 28 locus_tag: pHAGE-S4001-4500 label: 
                     pHAGE-S4001-4500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    4887..4966
                     /label=WPRE
                     /note="locus_tag: WPRE(1) label: WPRE(1) ApEinfo_revcolor: 
                     green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     LTR             5179..5500
                     /label=3' LTR
                     /label=3'\LTR
                     /note="vntifkey: 19 locus_tag: 3'\LTR label: 3'\LTR 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5179..5280
                     /label=HIV nef
                     /label=HIV\nef
                     /note="vntifkey: 21 note: HIV nef locus_tag: HIV\nef label:
                     HIV\nef ApEinfo_revcolor: #000000 ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5229..5243
                     /label=cPPT
                     /note="locus_tag: cPPT(1) label: cPPT(1) ApEinfo_revcolor: 
                     green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5229..5243
                     /label=HIV PPT
                     /label=HIV\PPT
                     /note="vntifkey: 21 note: PolyPurine Tract locus_tag: 
                     HIV\PPT label: HIV\PPT ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5246..5282
                     /label=dU3
                     /note="vntifkey: 21 note: 1st Half of deleted U3 locus_tag:
                     dU3 label: dU3 ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5302..5320
                     /label=dU3
                     /note="vntifkey: 21 note: 2nd Half of deleted U3 locus_tag:
                     dU3(1) label: dU3(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5321..5417
                     /label=HIV R
                     /label=HIV\R
                     /note="vntifkey: 21 locus_tag: HIV\R(1) label: HIV\R(1) 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5418..5500
                     /label=HIV U5
                     /label=HIV\U5
                     /note="vntifkey: 21 note: HIV U5 locus_tag: HIV\U5(1) 
                     label: HIV\U5(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     5532..5554
                     /label=pHAGE-S6001-6500
                     /note="vntifkey: 28 locus_tag: pHAGE-S6001-6500 label: 
                     pHAGE-S6001-6500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     promoter        5703..5737
                     /label=P-Bla
                     /note="vntifkey: 30 note: Beta Lactamase Promoter 
                     locus_tag: P-Bla label: P-Bla ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             5756..6595
                     /label=AMPr
                     /note="gene: AMPr product: beta-lactamase (mature form) 
                     vntifkey: 4 note: Ampicillin Resistance Gene locus_tag: 
                     AMPr label: AMPr ApEinfo_fwdcolor: pink ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             5756..6595
                     /label=AMPr
                     /note="gene: AMPr product: beta-lactamase (mature form) 
                     vntifkey: 4 note: Ampicillin Resistance Gene locus_tag: 
                     AMPr label: AMPr ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(5805..5828)
                     /label=pHAGE-A6050-5550
                     /note="vntifkey: 28 locus_tag: pHAGE-A6050-5550 label: 
                     pHAGE-A6050-5550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             5936..6595
                     /label=AmpR
                     /note="locus_tag: AmpR label: AmpR ApEinfo_fwdcolor: yellow
                     ApEinfo_revcolor: yellow ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             5936..6595
                     /label=AmpR
                     /note="locus_tag: AmpR label: AmpR ApEinfo_revcolor: yellow
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     6005..6029
                     /label=pHAGE-S6501-7000
                     /note="vntifkey: 28 locus_tag: pHAGE-S6501-7000 label: 
                     pHAGE-S6501-7000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(6274..6297)
                     /label=pHAGE-A6550-6050
                     /note="vntifkey: 28 locus_tag: pHAGE-A6550-6050 label: 
                     pHAGE-A6550-6050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     6525..6549
                     /label=pHAGE-S7001-7500
                     /note="vntifkey: 28 locus_tag: pHAGE-S7001-7500 label: 
                     pHAGE-S7001-7500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     rep_origin      6693..7375
                     /label=ColE1 origin
                     /note="locus_tag: ColE1 origin label: ColE1 origin 
                     ApEinfo_fwdcolor: gray50 ApEinfo_revcolor: gray50 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(6785..6806)
                     /label=pHAGE-A7050-6550
                     /note="vntifkey: 28 locus_tag: pHAGE-A7050-6550 label: 
                     pHAGE-A7050-6550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     rep_origin      6824..7357
                     /label=ORI
                     /note="gene: ORI product: ColE1 origin of replication 
                     vntifkey: 33 note: Bacterial Origin of Replication 
                     locus_tag: ORI label: ORI ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     7029..7053
                     /label=pHAGE-S7501-8000
                     /note="vntifkey: 28 locus_tag: pHAGE-S7501-8000 label: 
                     pHAGE-S7501-8000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(7296..7317)
                     /label=pHAGE-A7550-7050
                     /note="vntifkey: 28 locus_tag: pHAGE-A7550-7050 label: 
                     pHAGE-A7550-7050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     7532..7551
                     /label=pHAGE-S8001-8500
                     /note="vntifkey: 28 locus_tag: pHAGE-S8001-8500 label: 
                     pHAGE-S8001-8500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    7594..7717
                     /label=SV40 ori
                     /label=SV40\ori
                     /note="gene: SV40 ori vntifkey: 21 locus_tag: SV40\ori 
                     label: SV40\ori ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(7772..7798)
                     /label=pHAGE-A8050-7550
                     /note="vntifkey: 28 locus_tag: pHAGE-A8050-7550 label: 
                     pHAGE-A8050-7550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_binding    7838..7860
                     /label=LacO
                     /note="locus_tag: LacO label: LacO ApEinfo_revcolor: 
                     #6495ed ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} 
                     {} 0}width 5 offset 0"
     primer_bind     8037..8065
                     /label=pHAGE-S1-500
                     /note="vntifkey: 28 locus_tag: pHAGE-S1-500 label: 
                     pHAGE-S1-500 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgat
     2221 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     2281 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     2341 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2401 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2461 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2521 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2581 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2641 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2701 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2761 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     2821 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     2881 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     2941 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3001 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3061 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3121 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3181 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3241 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3301 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3361 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3421 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3481 cgtgagcggc caagggcgaa ttctgcagat atccatcaca ctggctagca tgtacaacat
     3541 gatggagacg gagctgaagc cgccgggccc gcagcaaact tcggggggcg gcggcggcaa
     3601 ctccaccgcg gcggcggccg gcggcaacca gaaaaacagc ccggaccgcg tcaagcggcc
     3661 catgaatgcc ttcatggtgt ggtcccgcgg gcagcggcgc aagatggccc aggagaaccc
     3721 caagatgcac aactcggaga tcagcaagcg cctgggcgcc gagtggaaac ttttgtcgga
     3781 gacggagaag cggccgttca tcgacgaggc taagcggctg cgagcgctgc acatgaagga
     3841 gcacccggat tataaatacc ggccccggcg gaaaaccaag acgctcatga agaaggataa
     3901 gtacacgctg cccggcgggc tgctggcccc cggcggcaat agcatggcga gcggggtcgg
     3961 ggtgggcgcc ggcctgggcg cgggcgtgaa ccagcgcatg gacagttacg cgcacatgaa
     4021 cggctggagc aacggcagct acagcatgat gcaggaccag ctgggctacc cgcagcaccc
     4081 gggcctcaat gcgcacggcg cagcgcagat gcagcccatg caccgctacg acgtgagcgc
     4141 cctgcagtac aactccatga ccagctcgca gacctacatg aacggctcgc ccacctacag
     4201 catgtcctac tcgcagcagg gcacccctgg catggctctt ggctccatgg gttcggtggt
     4261 caagtccgag gccagctcca gcccccctgt ggttacctct tcctcccact ccagggcgcc
     4321 ctgccaggcc ggggacctcc gggacatgat cagcatgtat ctccccggcg ccgaggtgcc
     4381 ggaacccgcc gcccccagca gacttcacat gtcccagcac taccagagcg gcccggtgcc
     4441 cggcacggcc attaacggca cactgcccct ctcacacatg attacgcgtg gcagcggcga
     4501 ctacaaggac cacgacggcg actacaagga ccacgacatc gactacaagg acgacgacga
     4561 caagtagtaa atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga
     4621 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4681 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4741 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4801 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4861 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4921 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     4981 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5041 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5101 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5161 ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5221 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5281 cacctgcagg acaggcgcgc cctgcttttt gcttgtactg ggtctctctg gttagaccag
     5341 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5401 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5461 tccctcagac ccttttagtc agtgtggaaa atctctagca cccgggcgat taaggaaagg
     5521 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5581 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5641 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5701 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5761 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5821 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5881 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5941 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     6001 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6061 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6121 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6181 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6241 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6301 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6361 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6421 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6481 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6541 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6601 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6661 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6721 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6781 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6841 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6901 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6961 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7021 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7081 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7141 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7201 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7261 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7321 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7381 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7441 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7501 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7561 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
     7621 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7681 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
     7741 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7801 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7861 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
     7921 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     7981 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     8041 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     8101 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
