LOCUS       Exported                8433 bp ds-DNA     circular SYN 02-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jan 31, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 
            pHAGE2-EF1alpha-Sox2.ape ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..636
                     /label=5' LTR
                     /label=5'\LTR
                     /note="gene: LTR vntifkey: 19 note: Long Terminal Repeat 
                     locus_tag: 5'\LTR label: 5'\LTR ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1..454
                     /label=HIV U3
                     /label=HIV\U3
                     /note="vntifkey: 21 note: Full Length HIV U3 locus_tag: 
                     HIV\U3 label: HIV\U3 ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(158..179)
                     /label=pHAGE-A0050-8050
                     /note="vntifkey: 28 locus_tag: pHAGE-A0050-8050 label: 
                     pHAGE-A0050-8050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     401..421
                     /label=pHAGE-S501-1000
                     /note="vntifkey: 28 locus_tag: pHAGE-S501-1000 label: 
                     pHAGE-S501-1000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    455..551
                     /label=HIV R
                     /label=HIV\R
                     /note="vntifkey: 21 note: HIV Repeat Region locus_tag: 
                     HIV\R label: HIV\R ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     polyA_site      550
                     /label=pA Site
                     /label=pA\Site
                     /note="vntifkey: 26 note: Exact base to which Poly-A is 
                     added locus_tag: pA\Site label: pA\Site ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     5'UTR           551..635
                     /label=5'\UTR
                     /note="vntifkey: 52 note: HIV 5' UTR locus_tag: 5'\UTR 
                     label: 5'\UTR ApEinfo_revcolor: pink ApEinfo_graphicformat:
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    552..636
                     /label=HIV U5
                     /label=HIV\U5
                     /note="vntifkey: 21 note: HIV U5 locus_tag: HIV\U5 label: 
                     HIV\U5 ApEinfo_revcolor: #000000 ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     polyA_signal    570..577
                     /label=G-T cluster
                     /label=G-T\cluster
                     /note="vntifkey: 25 note: GT Cluster shown to be necessary 
                     for Poly-A siteactivity by Cullen in J Virol. 1989 
                     January;63(1):421-424 locus_tag: G-T\cluster label: 
                     G-T\cluster ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    636..658
                     /label=PBS-K
                     /note="vntifkey: 21 note: tRNA binding site for Lysine tRNA
                     locus_tag: PBS-K label: PBS-K ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    637..1155
                     /label=PSI
                     /note="gene: psi product: pbs-gag vntifkey: 21 note: PSI 
                     Packaging Sequence locus_tag: PSI label: PSI 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     complement(667..690)
                     /label=pHAGE-A0550-0050
                     /note="vntifkey: 28 locus_tag: pHAGE-A0550-0050 label: 
                     pHAGE-A0550-0050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    697..806
                     /label=HIV-PSI
                     /note="vntifkey: 21 locus_tag: HIV-PSI label: HIV-PSI 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     stem_loop       697..731
                     /label=SL-1
                     /note="vntifkey: 39 note: PSI Stem loop 1 locus_tag: SL-1 
                     label: SL-1 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     stem_loop       736..754
                     /label=SL-2
                     /note="vntifkey: 39 note: PSI Stem loop 2 locus_tag: SL-2 
                     label: SL-2 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    744..745
                     /label=5' SD 289
                     /label=5'\SD\289
                     /note="vntifkey: 38 note: Major 5' Splice Donor 289 
                     locus_tag: 5'\SD\289 label: 5'\SD\289 ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     stem_loop       766..779
                     /label=SL-3
                     /note="vntifkey: 39 note: PSI Stem Loop 3 locus_tag: SL-3 
                     label: SL-3 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             790..1144
                     /label=HIV gag
                     /label=HIV\gag
                     /note="vntifkey: 4 note: HIV gag sequence from 336bp to 
                     688bp (Clone toPvuII site in HIV and filled in ClaI digest 
                     to take gagCDS out of frame) locus_tag: HIV\gag label: 
                     HIV\gag ApEinfo_fwdcolor: pink ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             790..1144
                     /label=HIV gag
                     /label=HIV\gag
                     /note="vntifkey: 4 note: HIV gag sequence from 336bp to 
                     688bp (Clone toPvuII site in HIV and filled in ClaI digest 
                     to take gagCDS out of frame) locus_tag: HIV\gag label: 
                     HIV\gag ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     stem_loop       793..806
                     /label=SL-4
                     /note="vntifkey: 39 note: PSI Stem Loop 4 locus_tag: SL-4 
                     label: SL-4 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    807..1144
                     /label=HR-ePSI
                     /note="vntifkey: 21 note: HIV gag sequence 351bp to 688bp 
                     locus_tag: HR-ePSI label: HR-ePSI ApEinfo_revcolor: #000000
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    833..834
                     /label=Fill-in
                     /note="vntifkey: 62 note: Filled in Cla Site from original 
                     HIV genome todisrupt gag polyprotein ORF locus_tag: Fill-in
                     label: Fill-in ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    853..855
                     /label=Stop Codon
                     /label=Stop\Codon
                     /note="vntifkey: 21 note: Stop codon in gag put in frame by
                     ClaI fill in locus_tag: Stop\Codon label: Stop\Codon 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     898..919
                     /label=pHAGE-S1001-1500
                     /note="vntifkey: 28 locus_tag: pHAGE-S1001-1500 label: 
                     pHAGE-S1001-1500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             1156..2013
                     /label=env
                     /note="vntifkey: 4 note: HIV env sequence from bp7168 to 
                     8025 (contains RRE)Cloned from BglII to BamHI out of HIV 
                     locus_tag: env label: env ApEinfo_fwdcolor: pink 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             1156..2013
                     /label=env
                     /note="vntifkey: 4 note: HIV env sequence from bp7168 to 
                     8025 (contains RRE)Cloned from BglII to BamHI out of HIV 
                     locus_tag: env label: env ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(1166..1189)
                     /label=pHAGE-A1050-0550
                     /note="vntifkey: 28 locus_tag: pHAGE-A1050-0550 label: 
                     pHAGE-A1050-0550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1245..1593
                     /label=RRE
                     /note="vntifkey: 21 note: Rev Responsive Element according 
                     to Belasco inMolecular Cell, Vol. 7, 603-614, March, 2001 
                     locus_tag: RRE(1) label: RRE(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1303..1536
                     /label=RRE
                     /note="gene: rre product: minimal RRE vntifkey: 21 note: 
                     Rev Responsive Element locus_tag: RRE label: RRE 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    1357
                     /label=RRE Mutation
                     /label=RRE\Mutation
                     /note="vntifkey: 62 note: This G is a C in the WT HIV-1 
                     genome locus_tag: RRE\Mutation label: RRE\Mutation 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     1391..1417
                     /label=pHAGE-S1501-2000
                     /note="vntifkey: 28 locus_tag: pHAGE-S1501-2000 label: 
                     pHAGE-S1501-2000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(1650..1678)
                     /label=pHAGE-A1550-1050
                     /note="vntifkey: 28 locus_tag: pHAGE-A1550-1050 label: 
                     pHAGE-A1550-1050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     1898..1922
                     /label=pHAGE-S2001-2500
                     /note="vntifkey: 28 locus_tag: pHAGE-S2001-2500 label: 
                     pHAGE-S2001-2500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    1911..1912
                     /label=SA 7925
                     /label=SA\7925
                     /note="vntifkey: 38 locus_tag: SA\7925 label: SA\7925 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2014..2027
                     /label=Junk
                     /note="vntifkey: 21 note: Linker Junk Sequence probably 
                     left over fromcloning locus_tag: Junk label: Junk 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2034..2211
                     /label=HIV cpPu (Trip)
                     /label=HIV\cpPu\(Trip)
                     /note="gene: cppt vntifkey: 21 note: Central Poly Purine 
                     Track locus_tag: HIV\cpPu\(Trip) label: HIV\cpPu\(Trip) 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2062..2076
                     /label=cPPT
                     /note="locus_tag: cPPT label: cPPT ApEinfo_revcolor: green 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(2084..2112)
                     /label=pHAGE-A2050-1550
                     /note="vntifkey: 28 locus_tag: pHAGE-A2050-1550 label: 
                     pHAGE-A2050-1550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    2188..2189
                     /label=SA 4459
                     /label=SA\4459
                     /note="vntifkey: 38 locus_tag: SA\4459 label: SA\4459 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2218..2614
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter label: EF1a promoter 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2367..2445
                     /label=EF-1 promoter
                     /note="locus_tag: EF-1 promoter label: EF-1 promoter 
                     ApEinfo_revcolor: #bab7be ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2616..3456
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter label: EF1a promoter 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3457..3485
                     /label=EF1a promoter
                     /note="locus_tag: EF1a promoter(1) label: EF1a promoter(1) 
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             3530..4771
                     /codon_start=1
                     /label=hSOX17
                     /translation="MSSPDAGYASDDQSQTQSALPAVMAGLGPCPWAESLSPIGDMKVK
                     GEAPANSGAPAGAAGRAKGESRIRRPMNAFMVWAKDERKRLAQQNPDLHNAELSKMLGK
                     SWKALTLAEKRPFVEEADRLRVQHMQDHPNYKYRPRRRKQVKRLKRVEGGFLHGLAEPQ
                     AAALGPEGGRVAMDGLGLQFPEQGFPAGPPLLPPHMGGHYRDCQSLGAPPLDGYPLPTP
                     DTSPLDGVDPDPAFFAAPMPGDCPAAGTYSYAQVSDYAGPPEPPAGPMHPRLGPEPAGP
                     SIPGLLAPPSALHVYYGAMGSPGAGGGRGFQMQPQHQHQHQHQHHPPGPGQPSPPPEAL
                     PCRDGTDPSQPAELLGEVDRTEFEQYLHFVCKPEMGLPYQGHDSGVNLPDSHGAISSVV
                     SDASSAVYYCNYPDV"
     CDS             3725..3961
                     /codon_start=1
                     /label=HMG
                     /translation="SRIRRPMNAFMVWAKDERKRLAQQNPDLHNAELSKMLGKSWKALT
                     LAEKRPFVEEADRLRVQHMQDHPNYKYRPRRRKQ"
     misc_feature    3866..3868
                     /label=Silent mutE48
     primer_bind     complement(3874..3905)
                     /label=hSOXE57R-R-3
     misc_feature    3887..3889
                     /label=Silent mutE55
     misc_feature    3893..3895
                     /label=E57D
     mat_peptide     4790..4855
                     /locus_tag="3xFLAG"
                     /label=3xFLAG
     misc_feature    4874..5465
                     /label=WPRE
                     /note="gene: WPRE vntifkey: 21 note: Woodchuck Hepatitis 
                     Virus PosttranscriptionalRegulatory Element locus_tag: WPRE
                     label: WPRE ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(4902..4936)
                     /label=pHAGE 3' CDS
                     /label=pHAGE\3'\CDS
                     /note="vntifkey: 28 locus_tag: pHAGE\3'\CDS label: 
                     pHAGE\3'\CDS ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer_bind     complement(4928..4948)
                     /label=WPRE-R
     primer_bind     complement(4980..5003)
                     /label=pHAGE-A3550-3050
                     /note="vntifkey: 28 locus_tag: pHAGE-A3550-3050 label: 
                     pHAGE-A3550-3050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     5133..5153
                     /label=pHAGE-S4001-4500
                     /note="vntifkey: 28 locus_tag: pHAGE-S4001-4500 label: 
                     pHAGE-S4001-4500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5178..5257
                     /label=WPRE
                     /note="locus_tag: WPRE(1) label: WPRE(1) ApEinfo_revcolor: 
                     green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     LTR             5470..5791
                     /label=3' LTR
                     /label=3'\LTR
                     /note="vntifkey: 19 locus_tag: 3'\LTR label: 3'\LTR 
                     ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5470..5571
                     /label=HIV nef
                     /label=HIV\nef
                     /note="vntifkey: 21 note: HIV nef locus_tag: HIV\nef label:
                     HIV\nef ApEinfo_revcolor: #000000 ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5520..5534
                     /label=cPPT
                     /note="locus_tag: cPPT(1) label: cPPT(1) ApEinfo_revcolor: 
                     green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5520..5534
                     /label=HIV PPT
                     /label=HIV\PPT
                     /note="vntifkey: 21 note: PolyPurine Tract locus_tag: 
                     HIV\PPT label: HIV\PPT ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5537..5573
                     /label=dU3
                     /note="vntifkey: 21 note: 1st Half of deleted U3 locus_tag:
                     dU3 label: dU3 ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5593..5611
                     /label=dU3
                     /note="vntifkey: 21 note: 2nd Half of deleted U3 locus_tag:
                     dU3(1) label: dU3(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    5612..5708
                     /label=HIV R
                     /label=HIV\R
                     /note="vntifkey: 21 locus_tag: HIV\R(1) label: HIV\R(1) 
                     ApEinfo_revcolor: #000000 ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5709..5791
                     /label=HIV U5
                     /label=HIV\U5
                     /note="vntifkey: 21 note: HIV U5 locus_tag: HIV\U5(1) 
                     label: HIV\U5(1) ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     5823..5845
                     /label=pHAGE-S6001-6500
                     /note="vntifkey: 28 locus_tag: pHAGE-S6001-6500 label: 
                     pHAGE-S6001-6500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     promoter        5994..6028
                     /label=P-Bla
                     /note="vntifkey: 30 note: Beta Lactamase Promoter 
                     locus_tag: P-Bla label: P-Bla ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             6047..6886
                     /label=AMPr
                     /note="gene: AMPr product: beta-lactamase (mature form) 
                     vntifkey: 4 note: Ampicillin Resistance Gene locus_tag: 
                     AMPr label: AMPr ApEinfo_fwdcolor: pink ApEinfo_revcolor: 
                     pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             6047..6886
                     /label=AMPr
                     /note="gene: AMPr product: beta-lactamase (mature form) 
                     vntifkey: 4 note: Ampicillin Resistance Gene locus_tag: 
                     AMPr label: AMPr ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(6096..6119)
                     /label=pHAGE-A6050-5550
                     /note="vntifkey: 28 locus_tag: pHAGE-A6050-5550 label: 
                     pHAGE-A6050-5550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     CDS             6227..6886
                     /label=AmpR
                     /note="locus_tag: AmpR label: AmpR ApEinfo_fwdcolor: yellow
                     ApEinfo_revcolor: yellow ApEinfo_graphicformat: arrow_data 
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     CDS             6227..6886
                     /label=AmpR
                     /note="locus_tag: AmpR label: AmpR ApEinfo_revcolor: yellow
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     6296..6320
                     /label=pHAGE-S6501-7000
                     /note="vntifkey: 28 locus_tag: pHAGE-S6501-7000 label: 
                     pHAGE-S6501-7000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(6565..6588)
                     /label=pHAGE-A6550-6050
                     /note="vntifkey: 28 locus_tag: pHAGE-A6550-6050 label: 
                     pHAGE-A6550-6050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     6816..6840
                     /label=pHAGE-S7001-7500
                     /note="vntifkey: 28 locus_tag: pHAGE-S7001-7500 label: 
                     pHAGE-S7001-7500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     rep_origin      6984..7666
                     /label=ColE1 origin
                     /note="locus_tag: ColE1 origin label: ColE1 origin 
                     ApEinfo_fwdcolor: gray50 ApEinfo_revcolor: gray50 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(7076..7097)
                     /label=pHAGE-A7050-6550
                     /note="vntifkey: 28 locus_tag: pHAGE-A7050-6550 label: 
                     pHAGE-A7050-6550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     rep_origin      7115..7648
                     /label=ORI
                     /note="gene: ORI product: ColE1 origin of replication 
                     vntifkey: 33 note: Bacterial Origin of Replication 
                     locus_tag: ORI label: ORI ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     7320..7344
                     /label=pHAGE-S7501-8000
                     /note="vntifkey: 28 locus_tag: pHAGE-S7501-8000 label: 
                     pHAGE-S7501-8000 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(7587..7608)
                     /label=pHAGE-A7550-7050
                     /note="vntifkey: 28 locus_tag: pHAGE-A7550-7050 label: 
                     pHAGE-A7550-7050 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     7823..7842
                     /label=pHAGE-S8001-8500
                     /note="vntifkey: 28 locus_tag: pHAGE-S8001-8500 label: 
                     pHAGE-S8001-8500 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_feature    7885..8008
                     /label=SV40 ori
                     /label=SV40\ori
                     /note="gene: SV40 ori vntifkey: 21 locus_tag: SV40\ori 
                     label: SV40\ori ApEinfo_revcolor: #000000 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     primer_bind     complement(8063..8089)
                     /label=pHAGE-A8050-7550
                     /note="vntifkey: 28 locus_tag: pHAGE-A8050-7550 label: 
                     pHAGE-A8050-7550 ApEinfo_revcolor: pink 
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 
                     0}width 5 offset 0"
     misc_binding    8129..8151
                     /label=LacO
                     /note="locus_tag: LacO label: LacO ApEinfo_revcolor: 
                     #6495ed ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} 
                     {} 0}width 5 offset 0"
     primer_bind     8328..8356
                     /label=pHAGE-S1-500
                     /note="vntifkey: 28 locus_tag: pHAGE-S1-500 label: 
                     pHAGE-S1-500 ApEinfo_revcolor: pink ApEinfo_graphicformat: 
                     arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgat
     2221 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     2281 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     2341 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2401 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2461 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2521 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     2581 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     2641 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     2701 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     2761 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     2821 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     2881 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     2941 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     3001 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     3061 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     3121 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     3181 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     3241 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     3301 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     3361 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     3421 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     3481 cgtgagcggc caagggcgaa ttctgcagat atccatcaca ctggctagca tgagcagccc
     3541 ggatgcggga tacgccagtg acgaccagag ccagacccag agcgcgctgc ccgcggtgat
     3601 ggccgggctg ggcccctgcc cctgggccga gtcgctgagc cccatcgggg acatgaaggt
     3661 gaagggcgag gcgccggcga acagcggagc accggccggg gccgcgggcc gagccaaggg
     3721 cgagtcccgt atccggcggc cgatgaacgc tttcatggtg tgggctaagg acgagcgcaa
     3781 gcggctggcg cagcagaatc cagacctgca caacgccgag ttgagcaaga tgctgggcaa
     3841 gtcgtggaag gcgctgacgc tggcggaaaa gcggcccttc gtggaggaag cagatcggct
     3901 gcgcgtgcag cacatgcagg accaccccaa ctacaagtac cggccgcggc ggcgcaagca
     3961 ggtgaagcgg ctgaagcggg tggagggcgg cttcctgcac ggcctggctg agccgcaggc
     4021 ggccgcgctg ggccccgagg gcggccgcgt ggccatggac ggcctgggcc tccagttccc
     4081 cgagcagggc ttccccgccg gcccgccgct gctgcctccg cacatgggcg gccactaccg
     4141 cgactgccag agtctgggcg cgcctccgct cgacggctac ccgttgccca cgcccgacac
     4201 gtccccgctg gacggcgtgg accccgaccc ggctttcttc gccgccccga tgcccgggga
     4261 ctgcccggcg gccggcacct acagctacgc gcaggtctcg gactacgctg gccccccgga
     4321 gcctcccgcc ggtcccatgc acccccgact cggcccagag cccgcgggtc cctcgattcc
     4381 gggcctcctg gcgccaccca gcgcccttca cgtgtactac ggcgcgatgg gctcgcccgg
     4441 ggcgggcggc gggcgcggct tccagatgca gccgcaacac cagcaccagc accagcacca
     4501 gcaccacccc ccgggccccg gacagccgtc gccccctccg gaggcactgc cctgccggga
     4561 cggcacggac cccagtcagc ccgccgagct cctcggggag gtggaccgca cggaatttga
     4621 acagtatctg cacttcgtgt gcaagcctga gatgggcctc ccctaccagg ggcatgactc
     4681 cggtgtgaat ctccccgaca gccacggggc catttcctcg gtggtgtccg acgccagctc
     4741 cgcggtatat tactgcaact atcctgacgt gattacgcgt ggcagcggcg actacaagga
     4801 ccacgacggc gactacaagg accacgacat cgactacaag gacgacgacg acaagtagta
     4861 aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4921 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4981 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     5041 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     5101 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5161 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5221 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     5281 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5341 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5401 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5461 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     5521 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tcacctgcag
     5581 gacaggcgcg ccctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     5641 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5701 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5761 cccttttagt cagtgtggaa aatctctagc acccgggcga ttaaggaaag ggctagatca
     5821 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5881 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5941 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6001 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6061 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6121 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6181 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6241 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     6301 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6361 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6421 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6481 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6541 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6601 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6661 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6721 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6781 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6841 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6901 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6961 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7021 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7201 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7441 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7681 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7741 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     7801 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     7861 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
     7921 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7981 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
     8041 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     8101 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     8161 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
     8221 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     8281 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     8341 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     8401 tatttgttaa atatgtacta caaacttagt agt
//
