LOCUS       GYRB_522                7689 bp    DNA     circular     31-JUL-2014
FEATURES             Location/Qualifiers
     promoter        209..225
                     /label="T7\promoter"
                     /vntifkey="29"
     misc_feature    209..228
                     /label="T7\promoter\priming\site"
                     /vntifkey="21"
     misc_feature    297..299
                     /label="Initial\ATG"
                     /vntifkey="21"
     misc_feature    300..317
                     /label="6xHis"
                     /vntifkey="21"
     CDS             318..359
                     /label="V5\epitope"
                     /vntifkey="21"
     CDS             360..380
                     /label="TEV\recognition\site"
                     /vntifkey="21"
     misc_feature    2387..2406
                     /label="T7\reverse\priming\site"
                     /vntifkey="21"
     CDS             2348..2476
                     /label="T7\transcription\termination\region"
                     /vntifkey="43"
     promoter        2781..2879
                     /label="bla\promoter"
                     /vntifkey="30"
     CDS             2880..3740
                     /label="Amp-R"
                     /vntifkey="22"
     rep_origin      3885..4558
                     /label="pBR322\ori"
                     /vntifkey="33"
     CDS             complement(4926..5117)
                     /label="ROP\ORF"
                     /vntifkey="4"
     CDS             complement(6429..7541)
                     /label="lacI\ORF"
                     /vntifkey="4"
     gene            396..2327
                     /label="GYRB_522"
                     /note="Insert"
     CDS             396..2327
                     /label="ORF"
ORIGIN
        1 CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA
       61 ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT
      121 ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA
      181 GAGGATCGAG ATCTCGATCC CGCGAAATTA ATACGACTCA CTATAGGGGA ATTGTGAGCG
      241 GATAACAATT CCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATGC
      301 ATCATCACCA TCACCATGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGG
      361 AAAACCTGTA TTTTCAGGGA ATTGATCCCT TCACCATGGT TACCGCACTG AGTGATGTGA
      421 ATAACACCGA TAATTATGGC GCAGGTCAGA TTCAGGTTCT GGAAGGTCTG GAAGCAGTTC
      481 GTAAACGTCC GGGTATGTAT ATTGGTAGCA CCAGCGAACG TGGTCTGCAT CATCTGGTTT
      541 GGGAAATTGT TGATAACAGC ATTGATGAAG CACTGGCAGG TTATGCAAAT CAGATTGAAG
      601 TGGTGATCGA GAAAGACAAC TGGATTAAAG TTACCGATAA TGGTCGTGGT ATTCCGGTTG
      661 ATATTCAAGA GAAAATGGGT CGTCCGGCAG TTGAAGTTAT TCTGACCGTT CTGCATGCCG
      721 GTGGCAAATT TGGTGGTGGT GGCTATAAAG TTAGCGGTGG CCTGCATGGT GTTGGTAGCA
      781 GCGTTGTTAA TGCACTGAGC CAGGATCTGG AAGTTTATGT TCATCGTAAC GAAACCATTT
      841 ATCACCAGGC CTATAAAAAG GGTGTTCCGC AGTTTGATCT GAAAGAAGTT GGCACCACCG
      901 ATAAAACCGG CACCGTTATT CGTTTTAAAG CAGATGGTGA AATCTTCACC GAAACCACCG
      961 TTTATAACTA TGAAACCCTG CAGCAGCGTA TTCGTGAACT GGCATTTCTG AATAAAGGCA
     1021 TTCAGATTAC CCTGCGTGAT GAACGTGACG AAGAAAATGT TCGTGAAGAT AGCTACCATT
     1081 ATGAAGGTGG CATTAAAAGC TATGTGGAAC TGCTGAACGA AAACAAAGAA CCGATTCATG
     1141 ATGAGCCGAT CTATATCCAT CAGAGCAAAG ATGATATCGA AGTGGAAATT GCCATCCAGT
     1201 ATAATAGCGG TTATGCCACC AATCTGCTGA CCTATGCAAA TAACATTCAT ACCTATGAAG
     1261 GCGGTACACA CGAAGATGGT TTTAAACGTG CACTGACCCG TGTTCTGAAT AGCTATGGTC
     1321 TGAGCAGCAA AATCATGAAA GAAGAAAAAG ATCGTCTGAG CGGTGAGGAT ACCCGTGAAG
     1381 GTATGACCGC AATTATTAGC ATTAAACATG GCGATCCTCA GTTTGAAGGT CAGACCAAAA
     1441 CCAAACTGGG TAATAGCGAA GTTCGTCAGG TTGTGGATAA ACTGTTTAGC GAACACTTTG
     1501 AGCGCTTTCT GTATGAAAAT CCGCAGGTTG CACGTACCGT TGTTGAAAAA GGTATTATGG
     1561 CAGCACGTGC CCGTGTTGCA GCAAAGAAAG CACGTGAAGT TACCCGTCGT AAAAGCGCAC
     1621 TGGATGTTGC AAGCCTGCCT GGTAAACTGG CAGATTGTAG CAGCAAATCA CCGGAAGAAT
     1681 GTGAAATTTT TCTGGTGGAA GGTGATAGTG CCGGTGGTAG TACCAAAAGC GGTCGTGATA
     1741 GTCGTACCCA GGCAATTCTG CCGCTGCGTG GTAAAATTCT GAATGTGGAA AAAGCACGTC
     1801 TGGATCGCAT TCTGAACAAT AATGAAATTC GCCAGATGAT TACCGCATTT GGCACCGGTA
     1861 TTGGTGGTGA TTTTGATCTG GCAAAAGCCC GTTATCATAA GATTGTGATT ATGACCGATG
     1921 CCGATGTTGA TGGTGCACAT ATTCGTACCC TGCTGCTGAT TTTCTTTTAT CGTTTTATGC
     1981 GTCCGCTGAT CGAAGCCGGT TATGTTTATA TTGCACAGCC TCCGCTGTAT AAACTGACCC
     2041 AGGGTAAACA GAAATACTAC GTGTATAATG ATCGCGAGCT GGACAAACTG AAAAGCGAAC
     2101 TGAATCCGAC ACCGAAATGG TCAATTGCAC GTTATAAAGG TCTGGGTGAA ATGAATGCAG
     2161 ATCAGCTGTG GGAAACCACC ATGAATCCGG AACATCGTGC ACTGCTGCAA GTTAAACTGG
     2221 AAGATGCAAT TGAAGCCGAT CAGACCTTTG AAATGCTGAT GGGTGATGTT GTGGAAAATC
     2281 GTCGCCAGTT TATTGAAGAT AATGCCGTTT ATGCCAACCT GGACTTTAAG GGCGAGCTCA
     2341 GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA
     2401 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA
     2461 GGAACTATAT CCGGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC
     2521 CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC
     2581 ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTAGCTTA
     2641 TCGATGATAA GCTGTCAAAC ATGAGAATTA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC
     2701 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT
     2761 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
     2821 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
     2881 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
     2941 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
     3001 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
     3061 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
     3121 GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
     3181 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
     3241 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
     3301 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
     3361 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
     3421 CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
     3481 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
     3541 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
     3601 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
     3661 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
     3721 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
     3781 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
     3841 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     3901 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     3961 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     4021 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     4081 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     4141 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     4201 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     4261 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC
     4321 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     4381 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     4441 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     4501 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     4561 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
     4621 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
     4681 AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCAATGGTG
     4741 CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG
     4801 CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA
     4861 CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC
     4921 ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA
     4981 TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG
     5041 AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT
     5101 TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG
     5161 ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG
     5221 TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA
     5281 ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC
     5341 CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT
     5401 TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC
     5461 GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA
     5521 GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC
     5581 CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC
     5641 GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA
     5701 TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG
     5761 TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG
     5821 CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG
     5881 TGACGATCAG CGGTCCAATG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA
     5941 GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC
     6001 CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA
     6061 ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT
     6121 CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC
     6181 CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA
     6241 AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA
     6301 TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG
     6361 CTCTCAAGGG CATCGGTCGA GATCCCGGTG CCTAATGAGT GAGCTAACTT ACATTAATTG
     6421 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA
     6481 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC
     6541 ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC
     6601 AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC
     6661 GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA CTACCGAGAT ATCCGCACCA
     6721 ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA GCGCCATCTG ATCGTTGGCA
     6781 ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG
     6841 GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA
     6901 TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG GCCCGCTAAC
     6961 AGCGCGATTT GCTGGTGACC CAATGCGACC AGATGCTCCA CGCCCAGTCG CGTACCGTCT
     7021 TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG AGACATCAAG AAATAACGCC
     7081 GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGCATCCT GGTCATCCAG CGGATAGTTA
     7141 ATGATCAGCC CACTGACGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCG
     7201 ACGCCGCTTC GTTCTACCAT CGACACCACC ACGCTGGCAC CCAGTTGATC GGCGCGAGAT
     7261 TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGGCCA GACTGGAGGT GGCAACGCCA
     7321 ATCAGCAACG ACTGTTTGCC CGCCAGTTGT TGTGCCACGC GGTTGGGAAT GTAATTCAGC
     7381 TCCGCCATCG CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG AAACGTGGCT GGCCTGGTTC
     7441 ACCACGCGGG AAACGGTCTG ATAAGAGACA CCGGCATACT CTGCGACATC GTATAACGTT
     7501 ACTGGTTTCA CATTCACCAC CCTGAATTGA CTCTCTTCCG GGCGCTATCA TGCCATACCG
     7561 CGAAAGGTTT TGCGCCATTC GATGGTGTCC GGGATCTCGA CGCTCTCCCT TATGCGACTC
     7621 CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA
     7681 TGGTGCATG
//
