LOCUS       pAAV-hSyn-FAS-GC        6007 bp    DNA     linear       05-JUL-2019
FEATURES             Location/Qualifiers
     misc_feature    2136..2723
                     /note="WPRE"
     misc_feature    2752..3230
                     /note="hGH_PA_terminator"
     misc_feature    3502..3808
                     /note="f1_origin"
     misc_feature    4076..4098
                     /note="pGEX_3_primer"
     misc_feature    4257..4285
                     /note="AmpR_promoter"
     misc_feature    4327..5187
                     /note="Ampicillin"
     misc_feature    5342..5961
                     /note="pBR322_origin"
     misc_feature    671..704
                     /note="loxFAS"
     misc_feature    713..2068
                     /note="GCaMP6f"
     PCR_primer      692..731
                     /pair=""
                     /primer="TATACGAAGTTGTGGCGCGCCATGGGTTCTCATCATCATC"
                     /current=0
     misc_feature    2076..2109
                     /note="loxFAS"
     PCR_primer      complement(2050..2089)
                     /pair=""
                     /primer="ATATACGAAGTTGTGCTAGCCGTTATCCTTCGCTGTCATC"
                     /current=0
     PCR_primer      complement(2203..2219)
                     /pair=""
                     /primer="GCAGCGTATCCACATAG"
                     /current=0
     PCR_primer      1100..1118
                     /pair=""
                     /primer="ATGGTCCTGCTGGAGTTCG"
                     /current=1
     misc_feature    713..2063
                     /note="GCaMP6f sequence confirmed"
     misc_feature    1661..1663
                     /note=""
     misc_feature    944..946
                     /note=""
     PCR_primer      189..208
                     /pair="hSyn"
                     /primer="GCAAGTGGGTTTTAGGACCA"
                     /current=0
     misc_feature    170..637
                     /note="hSyn promoter"
     PCR_primer      611..630
                     /pair="seq"
                     /primer="TCGTGTCGTGCCTGAGAGCG"
                     /current=0
     misc_feature    676..2160
                     /note="construct seq confirmed"
     source          1..6007
                     /dnas_title="pAAV-hSyn-FAS-GCaMP6f-WPRE-pA"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
      181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
      241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
      301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
      361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
      421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
      481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
      541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
      601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccgGAT CCTCTAGAGT
      661 CGACTCCGGA ACAACTTCGT ATATACCTTT CTATACGAAG TTGTGGCGCG CCatgggttc
      721 tcatcatcat catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga
      781 tctgtacgac gatgacgata aggatctcgc caccatggtc gactcatcac gtcgtaagtg
      841 gaataagaca ggtcacgcag tcagagctat aggtcggctg agctcactcg agaacgtcta
      901 tatcaaggcc gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat
      961 cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag aacaccccca tcggcgacgg
     1021 ccccgtgctg ctgcccgaca accactacct gagcgtgcag tccaaacttt cgaaagaccc
     1081 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1141 cggcatggac gagctgtaca agggcggtac cggagggagc atggtgagca agggcgagga
     1201 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1261 gttcagcgtg tccggcgagg gtgagggcga tgccacctac ggcaagctga ccctgaagtt
     1321 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1381 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1441 cgccatgccc gaaggctaca tccaggagcg caccatcttc ttcaaggacg acggcaacta
     1501 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1561 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaacctgcc
     1621 ggaccaactg actgaagagc agatcgcaga atttaaagag gaattctccc tatttgacaa
     1681 ggacggggat gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca
     1741 gaaccccaca gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg
     1801 cacaatcgac ttccctgagt tcctgacaat gatggcaaga aaaatgaaat acagggacac
     1861 ggaagaagaa attagagaag cgttcggtgt gtttgataag gatggcaatg gctacatcag
     1921 tgcagcagag cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt
     1981 tgatgaaatg atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt
     2041 tgtacaaatg atgacagcga aggataacGG CTAGCACAAC TTCGTATATA CCTTTCTATA
     2101 CGAAGTTGTG AATTCgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     2161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2521 tcggggaaat catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc
     2581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     2641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     2701 tccctttggg ccgcctcccc gcatcgatac cgagcgctgc tcgagagatc tacgggtggc
     2761 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     2821 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     2881 atattatggg gtggaggggg gtggtatgga gcaaggggca agttgggaag acaacctgta
     2941 gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt ggcacaatct tggctcactg
     3001 caatctccgc ctcctgggtt caagcgattc tcctgcctca gcctcccgag ttgttgggat
     3061 tccaggcatg catgaccagg ctcagctaat ttttgttttt ttggtagaga cggggtttca
     3121 ccatattggc caggctggtc tccaactcct aatctcaggt gatctaccca ccttggcctc
     3181 ccaaattgct gggattacag gcgtgaacca ctgctccctt ccctgtcctt ctgattttgt
     3241 aggtaaccac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
     3301 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     3361 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     3421 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     3481 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3541 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3601 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3661 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     3721 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3781 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat
     3841 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3901 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     3961 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4141 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4201 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4561 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4861 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6001 cacatgt
//

