LOCUS       split-GFP_PB_CMV        6589 bp DNA     circular SYN 04-FEB-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 4, 2024 from SnapGene 7.1.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1806..1942
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct(1)
                     /organism="synthetic DNA construct"
     source          5616..5689
                     /mol_type="other DNA"
                     /db_xref="taxon:1334104"
                     /organism="TRIP vector 
                     pPTK-Gal4-mPGK-Puro-IRES-eGFP-sNRP-pA-BC-Library"
     source          5616..5688
                     /mol_type="other DNA"
                     /db_xref="taxon:1334104"
                     /organism="TRIP vector 
                     pPTK-Gal4-mPGK-Puro-IRES-eGFP-sNRP-pA-BC-Library"
     source          5691..5708
                     /mol_type="other DNA"
                     /db_xref="taxon:1334104"
                     /organism="TRIP vector 
                     pPTK-Gal4-mPGK-Puro-IRES-eGFP-sNRP-pA-BC-Library"
     source          6319..6589
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /organism="synthetic DNA construct"
     CDS             complement(818..1675)
                     /codon_start=1
                     /label=Amp
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    1806..1809
                     /label=tata(1)
                     /note="tata;color: #cc0000"
     misc_feature    1806..1809
                     /label=tata
                     /note="tata;color: #cc0000"
     misc_feature    1810..1942
                     /label=5'-ITR
                     /note="5'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    1943..2081
                     /label=5LTRfix
     promoter        2103..2137
                     /label=EF1a Promoter
                     /label=EF1a\Promoter
     promoter        2138..3325
                     /label=EF1a Promoter from clontech promoter
                     /label=EF1a\Promoter from clontech
     misc_feature    3327..3332
                     /label=SalI
     misc_feature    3333..3338
                     /label=Kozak
                     /label=Kozaq
     misc_feature    3339..3963
                     /label=GFP
     misc_feature    3964..4273
                     /label=Minimal left repeat
     misc_feature    4282..4293
                     /label=MCS
     misc_feature    4294..4869
                     /locus_tag="CMV promoter"
                     /label=CMV promoter
     CDS             4876..5577
                     /codon_start=1
                     /locus_tag="TagBFP"
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     gap             5600..5615
                     /label=TRIP Barcode
                     /estimated_length=16
     misc_feature    5637..5658
                     /label=Capture sequence 1
     misc_feature    5659..5670
                     /label=iBC
     misc_feature    5691..5708
                     /label=polyA
                     /note="Homo sapiens soluble neuropilin-1 gene polyAsignal"
     misc_feature    5717..5954
                     /label=Minimal right repeat
     misc_feature    5955..6053
                     /label=GFP(1)
     misc_feature    6065..6080
                     /label=custom seq2
     misc_feature    6089..6310
                     /label=SV40 late polyA signal
                     /label=SV40
     misc_feature    6319..6360
                     /label=3'-proxITR
                     /note="3'-proxITR;modified region proximal to 3'-ITR -
                     contains CTCF binding sites;color: #cc0000"
     misc_feature    6361..6585
                     /label=3'-ITR
                     /note="3'-ITR;Sleeping Beauty inverted tandem repeat;color:
                     #cc00cc"
     misc_feature    6586..6589
                     /label=tata(2)
                     /note="tata;color: #cc0000"
ORIGIN
        1 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
       61 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      121 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      181 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      241 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      301 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      361 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      421 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      481 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      541 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      601 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      661 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      721 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      781 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      841 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      901 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
      961 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1021 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1081 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     1141 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1201 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1261 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1321 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1381 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1441 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1501 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1561 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1621 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1681 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1741 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1801 caccttatac agttgaagtc ggaagtttac atacacttaa gttggagtca ttaaaactcg
     1861 tttttcaact actccacaaa tttcttgtta acaaacaata gttttggcaa gtcagttagg
     1921 acatctactt tgtgcatgac acaagtcatt tttccaacaa ttgtttacag acagattatt
     1981 tcacttataa ttcactgtat cacaattcca gtgggtcaga agtttacata cactaagttc
     2041 gactcctctg cagaatgcgg cgatgtttcg gtaaggggtc cgcgatcgcc ctaaggacgc
     2101 gtccgctctc gtcatcactg aggtggagaa gagcatgcgt gaggctccgg tgcccgtcag
     2161 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     2221 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     2281 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     2341 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     2401 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac gcccctggct
     2461 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     2521 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcttg ggcgctgggg
     2581 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2641 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2701 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2761 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2821 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     2881 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     2941 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3001 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3061 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3121 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3181 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3241 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3301 ttttcttcca tttcaggtgt cgtgaagtcg acgccaccat ggtgagcaag ggcgaggagc
     3361 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3421 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3481 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3541 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3601 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3661 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3721 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3781 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     3841 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3901 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgcct
     3961 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
     4021 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     4081 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
     4141 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
     4201 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
     4261 gttatagata tcaggcgcgc caagcttgga tcctagtaat caattacggg gtcattagtt
     4321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4621 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4681 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4741 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4801 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg
     4861 aaccgtcaga ccggtatgag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg
     4921 gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac
     4981 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     5041 gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc
     5101 atccccgact tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca
     5161 tacgaggacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     5221 atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag
     5281 aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa
     5341 ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag
     5401 accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg
     5461 gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag
     5521 gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct taattgagag
     5581 ctctcgcttc gagtctagan nnnnnnnnnn nnnnnctcga gttgtggccg gcccttgctt
     5641 taaggccggt cctagcaaag ctggtcggaa gttgggaaaa ccctggcgtt aaataaaata
     5701 cgaaatgagc ggccgctaaa agttttgtta ctttatagaa gaaattttga gtttttgttt
     5761 ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat tagtatgtaa
     5821 gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc gatatacaga
     5881 ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt cacaatatga
     5941 ttatctttct agggttaagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6001 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taataagttt
     6061 aaactcagtg tccatagcga cgccggcgca gacatgataa gatacattga tgagtttgga
     6121 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     6181 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     6241 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     6301 aaatgtggta ttaattaagg accccttacc gaaacatcgc cgcattctgc agaggagtcg
     6361 agtgtatgta aacttctgac ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa
     6421 tcattctctc tactattatt ctgatatttc acattcttaa aataaagtgg tgatcctaac
     6481 tgacctaaga cagggaattt ttactaggat taaatgtcag gaattgtgaa aaagtgagtt
     6541 taaatgtatt tggctaaggt gtatgtaaac ttccgacttc aactgtata
//
