LOCUS       plasmid                 2899 bp    DNA     circular SYN 13-FEB-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(1229..2044)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             3..716
                     /note="pLannotate"
                     /label="Superfolder GFP"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2166..2755
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.2"
                     /fragment="False"
                     /other="rep_origin"
     terminator      1164..1211
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     terminator      1027..1073
                     /note="pLannotate"
                     /label="rrnB T1 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     promoter        2783..2801
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     ncRNA           908..1023
                     /note="pLannotate"
                     /label="5S rRNA"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="97.5"
                     /fragment="False"
                     /other="ncRNA"
     CDS             1107..1156
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
       61 gtgatgttaa tgggcacaaa ttttctgtcc gtggagaggg tgaaggtgat gctacaaacg
      121 gaaaactcac ccttaaattt atttgcacta ctggaaaact acctgttccg tggccaacac
      181 ttgtcactac tctgacctat ggtgttcaat gcttttcccg ttatccggat cacatgaaac
      241 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt
      301 tcaaagatga cgggacctac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg
      361 ttaatcgtat cgagttaaag ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      421 aactcgagta caactttaac tcacacaatg tatacatcac ggcagacaaa caaaagaatg
      481 gaatcaaagc taacttcaaa attcgccaca acgttgaaga tggttccgtt caactagcag
      541 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      601 acctgtcgac acaatctgtc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc
      661 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagctc tacaaataag
      721 gatctgaagc ttgggcccga acaaaaactc atctcagaag aggatctgaa tagcgccgtc
      781 gaccatcatc atcatcatca ttgagtttaa acggtctcca gcttggctgt tttggcggat
      841 gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
      901 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
      961 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     1021 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1081 ttgtcggtga actggatcgt cgaccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     1141 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     1201 ggggtttttt gctgaaagcc aattctgatt agaaaaactc atcgagcatc aaatgaaact
     1261 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     1321 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     1381 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     1441 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca
     1501 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     1561 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     1621 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     1681 aaacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     1741 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     1801 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     1861 caacgctacc tttgccatgt ttaagaaaca actctggcgc atcgggcttc ccatacaatc
     1921 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     1981 cagcatccat gttggaattt aatcgcggct tcgagcaaga cgtttcccgt tgaatatggc
     2041 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     2101 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtga gatcaaagga
     2161 tcttcttgag atcctttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     2221 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     2281 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     2341 ccacttcaag aactctgtag caccgcctac atacatcgct ctgctaatcc tgttaccagt
     2401 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     2461 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2521 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     2581 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     2641 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     2701 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2761 cagcaacgcg atcccgcgaa attaatacga ctcactatag ggggatacta tttaacgaag
     2821 agacataact ttgttgggtc ggactttaga acagaggaga taaagatgga cccaacaaag
     2881 aacctggcgg cagcgcaaa
//
