LOCUS       pPOM032_P4_tSynt        2792 bp DNA     circular SYN 25-AUG-2023
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   IOCB Prague
  TITLE     Direct Submission
  JOURNAL   Exported Aug 25, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     328..344
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    410..486
                     /label=tSynth30
     primer_bind     complement(571..587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    595..611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(619..649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    664..685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(973..1561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1732..2592)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2593..2697)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
       61 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
      121 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
      181 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      241 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      301 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattg gagatcggta
      361 cttcgcgaat gcgtcgagat ctagacatcc gtctcatcgg tctcaatcct aactcgagtt
      421 tttttttata tatatatata tatatatata aactcattta cttatgtagg aataaatttt
      481 ttcaaagctg tgagacctga gacgcatatg cacatcggat gccgggaccg acgagtgcag
      541 aggcgtgcaa gcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
      601 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
      661 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      721 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      781 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      841 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      901 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      961 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1021 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1081 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1141 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1201 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1261 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1321 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1381 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1441 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1501 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1561 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1621 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1681 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1741 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1801 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1861 aatgataccg cgagatccac gctcaccggc tccagattta tcagcaataa accagccagc
     1921 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1981 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2041 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2101 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2161 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2221 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2281 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2341 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2401 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2461 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2521 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2581 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2701 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2761 taaaaatagg cgtatcacga ggccctttcg tc
//
