LOCUS       Exported                9877 bp ds-DNA     circular SYN 06-FEB-2024
DEFINITION  p2106-pHAGE-Ef1aL-YAP5SA-UBC-GFP
            
            Claire Burgess, 02/19/2019
            
            Using pHAGE1 dual promoter backbone (Ef1aL-dsRed-UBC-GFP), YAP5SA 
            was PCR cloned out of pLVX-puro-NT3F-YAP2A-5SA courtesy of the 
            Varelas lab. Cut sites for NotI and BglII were added to either end, 
            and cloned into the NotI to BamhI sites in the backbone. The YAP5SA 
            sequence and ligation sites were sequenced.
ACCESSION   .
VERSION     .
KEYWORDS    p2706-pHAGE-Ef1aL-YAP5SA-UBC-GFP-W
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9877)
  AUTHORS   Kotton Lab 3
  TITLE     Direct Submission
  JOURNAL   Exported Feb 6, 2024 from SnapGene 2.7.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9877
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..636
                     /gene="LTR"
                     /note="LTR"
                     /note="Long Terminal Repeat"
     misc_feature    1..453
                     /note="HIV U3"
                     /note="Full Length HIV U3"
     primer_bind     complement(158..179)
                     /note="pHAGE-A0050-8050"
     primer_bind     401..421
                     /note="pHAGE-S501-1000"
     misc_feature    454..551
                     /note="HIV R"
                     /note="HIV Repeat Region"
     misc_feature    552..636
                     /note="HIV U5"
     misc_feature    636..658
                     /note="PBS-K"
                     /note="tRNA binding site for Lysine tRNA"
     misc_feature    637..1155
                     /gene="psi"
                     /note="PSI"
                     /note="PSI Packaging Sequence"
     primer_bind     complement(667..690)
                     /note="pHAGE-A0550-0050"
     misc_feature    697..806
                     /note="HIV-PSI"
     misc_feature    807..1144
                     /note="HR-ePSI"
     primer_bind     898..919
                     /note="pHAGE-S1001-1500"
     primer_bind     complement(1166..1189)
                     /note="pHAGE-A1050-0550"
     misc_feature    1303..1536
                     /gene="rre"
                     /note="RRE"
                     /note="Rev Responsive Element"
     primer_bind     1391..1417
                     /note="pHAGE-S1501-2000"
     primer_bind     complement(1650..1678)
                     /note="pHAGE-A1550-1050"
     primer_bind     1898..1922
                     /note="pHAGE-S2001-2500"
     misc_feature    2034..2211
                     /gene="cppt"
                     /note="HIV cpPu (Trip)"
                     /note="Central Poly Purine Track"
     primer_bind     complement(2084..2112)
                     /note="pHAGE-A2050-1550"
     misc_feature    2218..3402
                     /note="Human EF1a Promoter"
                     /note="Human Elongation Factor 1 alpha promoter"
     intron          2454..3045
                     /note="Human EF1a Intron"
     misc_feature    2531
                     /note="EF1a Mutation"
                     /note="Missing GCCC according to Genbank and Celera 
                     sequences"
     misc_feature    3411..3446
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    3480..5090
                     /note="YAP-5SA"
                     /note="portion cloned from Varelas lab 
                     pLVX-Puro-NT3F-YAP2A-5SA plasmid"
     CDS             3480..3545
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5092..5116)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5122..5532
                     /note="UBCp"
     CDS             5534..6253
                     /note="eGFP"
     misc_feature    6266..6857
                     /gene="WPRE"
                     /note="WPRE"
                     /note="Woodchuck Hepatitis Virus Posttranscriptional 
                     Regulatory Element"
     primer_bind     complement(6294..6328)
                     /note="pHAGE 3' CDS"
     primer_bind     complement(6372..6395)
                     /note="pHAGE-A3550-3050"
     primer_bind     6525..6545
                     /note="pHAGE-S4001-4500"
     primer_bind     complement(6853..6875)
                     /note="pHAGE-A4050-3550"
     misc_feature    6863..6951
                     /note="HIV nef"
     misc_feature    6928..7163
                     /gene="dU3 LTR"
                     /note="dU3 LTR"
                     /note="Deleted U3 Long-Terminal Repeat"
     misc_feature    6965..7080
                     /note="HIV R"
                     /note="HIV Repeat Region"
     modified_base   6976
                     /note="Difference from 5' LTR"
                     /note="This T is a C in the 5' LTR"
     misc_feature    7081..7163
                     /note="HIV U5"
     primer_bind     7161..7192
                     /note="pHAGE-S4501-5000"
     misc_feature    7164..7234
                     /note="3' Flank"
                     /note="Chromosomal 3' Flanking Sequence carried over from 
                     original HIV integration site"
     primer_bind     7267..7289
                     /note="pHAGE-S6001-6500"
     promoter        7438..7472
                     /note="P-Bla"
                     /note="Beta Lactamase Promoter"
     CDS             7491..8330
                     /gene="AMPr"
                     /note="AMPr"
                     /note="Ampicillin Resistance Gene"
     primer_bind     complement(7540..7563)
                     /note="pHAGE-A6050-5550"
     primer_bind     7740..7764
                     /note="pHAGE-S6501-7000"
     primer_bind     complement(8009..8032)
                     /note="pHAGE-A6550-6050"
     primer_bind     8260..8284
                     /note="pHAGE-S7001-7500"
     primer_bind     complement(8520..8541)
                     /note="pHAGE-A7050-6550"
     rep_origin      8559..9092
                     /gene="ORI"
                     /note="ORI"
                     /note="Bacterial Origin of Replication"
     primer_bind     8764..8788
                     /note="pHAGE-S7501-8000"
     primer_bind     complement(9031..9052)
                     /note="pHAGE-A7550-7050"
     primer_bind     9267..9286
                     /note="pHAGE-S8001-8500"
     misc_feature    9329..9452
                     /gene="SV40 ori"
                     /note="SV40 ori"
     primer_bind     complement(9507..9533)
                     /note="pHAGE-A8050-7550"
     primer_bind     9772..9800
                     /note="pHAGE-S1-500"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc aattctgcag
     3421 tcgacggtac cgcgggcccg ggatccgacc ctaggccgac gcgtctcgag ggtaccatgg
     3481 actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag gatgacgatg
     3541 acaagcttac aagtttgtac aaaaaagttg ccaccatgga tcccgggcag cagccgccgc
     3601 ctcaaccggc cccccagggc caagggcagc cgccttcgca gcccccgcag gggcagggcc
     3661 cgccgtccgg acccgggcaa ccggcacccg cggcgaccca ggcggcgccg caggcacccc
     3721 ccgccgggca tcagatcgtg cacgtccgcg gggacgcgga gaccgacctg gaggcgctct
     3781 tcaacgccgt catgaacccc aagacggcca acgtgcccca gaccgtgccc atgaggctcc
     3841 ggaagctgcc cgactccttc ttcaagccgc cggagcccaa atcccactcc cgacaggccg
     3901 ctactgatgc aggcactgca ggagccctga ctccacagca tgttcgagct catgcctctc
     3961 cagcttctct gcagttggga gctgtttctc ctgggacact gacccccact ggagtagtct
     4021 ctggcccagc agctacaccc acagctcagc atcttcgaca gtctgctttt gagatacctg
     4081 atgatgtacc tctgccagca ggttgggaga tggcaaagac atcttctggt cagagatact
     4141 tcttaaatca catcgatcag acaacaacat ggcaggaccc caggaaggcc atgctgtccc
     4201 agatgaacgt cacagccccc accagtccac cagtgcagca gaatatgatg aactcggctt
     4261 caggtcctct tcctgatgga tgggaacaag ccatgactca ggatggagaa atttactata
     4321 taaaccataa gaacaagacc acctcttggc tagacccaag gcttgaccct cgttttgcca
     4381 tgaaccagag aatcagtcag agtgctccag tgaaacagcc accacccctg gctccccaga
     4441 gcccacaggg aggcgtcatg ggtggcagca actccaacca gcagcaacag atgcgactgc
     4501 agcaactgca gatggagaag gagaggctgc ggctgaaaca gcaagaactg cttcggcagg
     4561 caatgcggaa tatcaatccc agcacagcaa attctccaaa atgtcaggag ttagccctgc
     4621 gtagccagtt accaacactg gagcaggatg gtgggactca aaatccagtg tcttctcccg
     4681 ggatgtctca ggaattgaga acaatgacga ccaatagctc agatcctttc cttaacagtg
     4741 gcacctatca ctctcgagat gaggctacag acagtggact aagcatgagc agctacagtg
     4801 tccctcgaac cccagatgac ttcctgaaca gtgtggatga gatggataca ggtgatacta
     4861 tcaaccaaag caccctgccc tcacagcaga accgtttccc agactacctt gaagccattc
     4921 ctgggacaaa tgtggacctt ggaacactgg aaggagatgg aatgaacata gaaggagagg
     4981 agctgatgcc aagtctgcag gaagctttga gttctgacat ccttaatgac atggagtctg
     5041 ttttggctgc caccaagcta gataaagaaa gctttcttac atggttataa tacccaactt
     5101 tcttgtacaa agtggtgtga agatccggcc tccgcgccgg gttttggcgc ctcccgcggg
     5161 cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga
     5221 tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc
     5281 ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt
     5341 ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg
     5401 atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct
     5461 agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca
     5521 cttggccgcc catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5581 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5641 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5701 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     5761 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     5821 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5881 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     5941 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     6001 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     6061 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     6121 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     6181 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     6241 gctgtacaag taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     6301 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6361 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6421 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6481 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6541 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6601 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6661 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6721 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6781 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6841 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     6901 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6961 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7141 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7201 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
     7261 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7321 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7381 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7441 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7501 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7561 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7621 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7681 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     7741 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7801 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7861 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7921 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7981 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8041 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8101 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8161 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8221 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8281 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8341 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8401 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8461 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8521 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8581 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8641 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8701 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8761 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8821 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8881 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8941 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9001 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9061 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9121 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9181 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9241 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9301 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
     9361 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9421 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
     9481 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     9541 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     9601 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
     9661 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     9721 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     9781 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     9841 attgtatttg ttaaatatgt actacaaact tagtagt
//
