LOCUS       Exported                8839 bp ds-DNA     circular SYN 06-FEB-2024
DEFINITION  p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP-W
            
            Claire Burgess, 8/28/2019
            
            tagBFP was PCR cloned from pHAGE-Ef1aL-tagBFP with added cut sites 
            NdeI and ClaI into the second gene site of 
            pHAGE-Ef1aL-dsRed-UBC-eGFP plasmid backbone. TagBFP insertion was 
            sequenced. 
            
            Note for this history of this plasmid in Snapgene: the backbone 
            plasmid we had in the freezer already contained Ef1aL promoter, but 
            we did not have a corresponding Snapgene file(the only one had the 
            short ef1a promoter, as original file shows in the history. Thus 
            Ef1aL promoter was added from another plasmid in snapgene to make 
            this file match the sequenced plasmid).
ACCESSION   .
VERSION     .
KEYWORDS    p2707-pHAGE EF1aL-DsRed-UBC-tagBFP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   DEREK LIBERTI
  TITLE     Direct Submission
  JOURNAL   Exported Feb 6, 2024 from SnapGene 2.7.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..636
                     /gene="LTR"
                     /note="LTR"
                     /note="Long Terminal Repeat"
     misc_feature    1..453
                     /note="HIV U3"
                     /note="Full Length HIV U3"
     primer_bind     complement(158..179)
                     /note="pHAGE-A0050-8050"
     primer_bind     401..421
                     /note="pHAGE-S501-1000"
     misc_feature    454..551
                     /note="HIV R"
                     /note="HIV Repeat Region"
     misc_feature    552..636
                     /note="HIV U5"
     misc_feature    636..658
                     /note="PBS-K"
                     /note="tRNA binding site for Lysine tRNA"
     misc_feature    637..1155
                     /gene="psi"
                     /note="PSI"
                     /note="PSI Packaging Sequence"
     primer_bind     complement(667..690)
                     /note="pHAGE-A0550-0050"
     misc_feature    697..806
                     /note="HIV-PSI"
     misc_feature    807..1144
                     /note="HR-ePSI"
     primer_bind     898..919
                     /note="pHAGE-S1001-1500"
     primer_bind     complement(1166..1189)
                     /note="pHAGE-A1050-0550"
     misc_feature    1303..1536
                     /gene="rre"
                     /note="RRE"
                     /note="Rev Responsive Element"
     primer_bind     1391..1417
                     /note="pHAGE-S1501-2000"
     primer_bind     complement(1650..1678)
                     /note="pHAGE-A1550-1050"
     primer_bind     1898..1922
                     /note="pHAGE-S2001-2500"
     misc_feature    2034..2211
                     /gene="cppt"
                     /note="HIV cpPu (Trip)"
                     /note="Central Poly Purine Track"
     primer_bind     complement(2084..2112)
                     /note="pHAGE-A2050-1550"
     misc_feature    2218..3402
                     /note="Human EF1a Promoter"
                     /note="Human Elongation Factor 1 alpha promoter"
     intron          2454..3045
                     /note="Human EF1a Intron"
     misc_feature    2531
                     /note="EF1a Mutation"
                     /note="Missing GCCC according to Genbank and Celera 
                     sequences"
     CDS             3411..4089
                     /note="dsRed express"
     misc_feature    4091..4501
                     /note="UBCp"
     CDS             4509..5210
                     /codon_start=1
                     /note="TagBFP"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    5228..5819
                     /gene="WPRE"
                     /note="WPRE"
                     /note="Woodchuck Hepatitis Virus Posttranscriptional 
                     Regulatory Element"
     primer_bind     complement(5256..5290)
                     /note="pHAGE 3' CDS"
     primer_bind     complement(5334..5357)
                     /note="pHAGE-A3550-3050"
     primer_bind     5487..5507
                     /note="pHAGE-S4001-4500"
     primer_bind     complement(5815..5837)
                     /note="pHAGE-A4050-3550"
     misc_feature    5825..5913
                     /note="HIV nef"
     misc_feature    5890..6125
                     /gene="dU3 LTR"
                     /note="dU3 LTR"
                     /note="Deleted U3 Long-Terminal Repeat"
     misc_feature    5927..6042
                     /note="HIV R"
                     /note="HIV Repeat Region"
     modified_base   5938
                     /note="Difference from 5' LTR"
                     /note="This T is a C in the 5' LTR"
     misc_feature    6043..6125
                     /note="HIV U5"
     primer_bind     6123..6154
                     /note="pHAGE-S4501-5000"
     misc_feature    6126..6196
                     /note="3' Flank"
                     /note="Chromosomal 3' Flanking Sequence carried over from 
                     original HIV integration site"
     primer_bind     6229..6251
                     /note="pHAGE-S6001-6500"
     promoter        6400..6434
                     /note="P-Bla"
                     /note="Beta Lactamase Promoter"
     CDS             6453..7292
                     /gene="AMPr"
                     /note="AMPr"
                     /note="Ampicillin Resistance Gene"
     primer_bind     complement(6502..6525)
                     /note="pHAGE-A6050-5550"
     primer_bind     6702..6726
                     /note="pHAGE-S6501-7000"
     primer_bind     complement(6971..6994)
                     /note="pHAGE-A6550-6050"
     primer_bind     7222..7246
                     /note="pHAGE-S7001-7500"
     primer_bind     complement(7482..7503)
                     /note="pHAGE-A7050-6550"
     rep_origin      7521..8054
                     /gene="ORI"
                     /note="ORI"
                     /note="Bacterial Origin of Replication"
     primer_bind     7726..7750
                     /note="pHAGE-S7501-8000"
     primer_bind     complement(7993..8014)
                     /note="pHAGE-A7550-7050"
     primer_bind     8229..8248
                     /note="pHAGE-S8001-8500"
     misc_feature    8291..8414
                     /gene="SV40 ori"
                     /note="SV40 ori"
     primer_bind     complement(8469..8495)
                     /note="pHAGE-A8050-7550"
     primer_bind     8734..8762
                     /note="pHAGE-S1-500"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc catggcctcc
     3421 tccgaggacg tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac
     3481 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     3541 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     3601 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
     3661 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     3721 gtggtgaccg tgacccagga ctcctccctg caggacggct ccttcatcta caaggtgaag
     3781 ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc agaagaagac tatgggctgg
     3841 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag
     3901 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc
     3961 aagaagcccg tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc
     4021 cacaacgagg actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg
     4081 ttcctgtagg gatccggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc
     4141 tcacggcgag cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     4201 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     4261 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     4321 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     4381 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     4441 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggccgccc
     4501 atatggccat gagcgagctg attaaggaga acatgcacat gaagctgtac atggagggca
     4561 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     4621 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     4681 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
     4741 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     4801 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     4861 acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac
     4921 tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa
     4981 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc aagaccacat
     5041 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
     5101 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
     5161 tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattaa aggatatcga
     5221 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     5281 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     5341 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     5401 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5461 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5521 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5581 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     5641 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5701 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5761 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     5821 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5881 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     5941 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6001 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6061 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6121 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6181 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     6241 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     6301 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6361 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6421 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6481 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6541 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6601 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6661 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6721 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6781 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6841 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6901 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6961 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     7021 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     7081 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     7141 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     7201 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     7261 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7321 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7381 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7441 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7501 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7561 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7621 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7681 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7741 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7801 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7861 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7921 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7981 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     8041 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     8101 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     8161 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     8221 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     8281 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8341 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8401 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     8461 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     8521 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     8581 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8641 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     8701 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     8761 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     8821 gtactacaaa cttagtagt
//
