LOCUS       Exported                9489 bp ds-DNA     circular SYN 08-DEC-2023
DEFINITION  p2711-PhAGE2-Ef1aL-TAZ4SA-tagBFP-loxP
            
            Claire Burgess, 08/21/2020
            
            TAZ4SA was PCR cloned out of pLVX-Tight-Puro-3F-TAZ4SA courtesy of 
            the Varelas lab. Cut sites for NotI and BamHI were added to either 
            end, and cloned into the NotI to BamhI sites in the 
            pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP backbone cut at NotI and BamHI. 
            Full coverage of insertion sites and TAZ4SA were sequenced.
ACCESSION   .
VERSION     .
KEYWORDS    p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   DEREK LIBERTI
  TITLE     Direct Submission
  JOURNAL   Exported Feb 6, 2024 from SnapGene 2.7.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..636
                     /gene="LTR"
                     /note="5' LTR"
                     /note="Long Terminal Repeat"
     misc_feature    1..454
                     /note="HIV U3"
                     /note="Full Length HIV U3"
     primer_bind     complement(158..179)
                     /note="pHAGE-A0050-8050"
     primer_bind     401..421
                     /note="pHAGE-S501-1000"
     misc_feature    455..551
                     /note="HIV R"
                     /note="HIV Repeat Region"
     polyA_site      550
                     /note="pA Site"
                     /note="Exact base to which Poly-A is added"
     5'UTR           551..635
                     /note="HIV 5' UTR"
     misc_feature    552..636
                     /note="HIV U5"
     polyA_signal    570..577
                     /note="G-T cluster"
                     /note="GT Cluster shown to be necessary for Poly-A site 
                     activity by Cullen in J Virol. 1989 January; 63(1):421-424"
     misc_feature    636..658
                     /note="PBS-K"
                     /note="tRNA binding site for Lysine tRNA"
     misc_feature    637..1155
                     /gene="psi"
                     /note="PSI"
                     /note="PSI Packaging Sequence"
     primer_bind     complement(667..690)
                     /note="pHAGE-A0550-0050"
     misc_feature    697..806
                     /note="HIV-PSI"
     stem_loop       697..731
                     /note="SL-1"
                     /note="PSI Stem loop 1"
     stem_loop       736..754
                     /note="SL-2"
                     /note="PSI Stem loop 2"
     misc_feature    744..745
                     /note="5' SD 289"
                     /note="Major 5' Splice Donor 289"
     stem_loop       766..779
                     /note="SL-3"
                     /note="PSI Stem Loop 3"
     CDS             790..1144
                     /note="HIV gag"
                     /note="HIV gag sequence from 336bp to 688bp (Clone to PvuII
                     site in HIV and filled in ClaI digest to take gag CDS out 
                     of frame)"
     stem_loop       793..806
                     /note="SL-4"
                     /note="PSI Stem Loop 4"
     misc_feature    807..1144
                     /note="HR-ePSI"
                     /note="HIV gag sequence 351bp to 688bp"
     misc_feature    833..834
                     /note="Fill-in"
                     /note="Filled in Cla Site from original HIV genome to 
                     disrupt gag polyprotein ORF"
     misc_feature    853..855
                     /note="Stop Codon"
                     /note="Stop codon in gag put in frame by ClaI fill in"
     primer_bind     898..919
                     /note="pHAGE-S1001-1500"
     CDS             1156..2013
                     /note="env"
                     /note="HIV env sequence from bp7168 to 8025 (contains RRE) 
                     Cloned from BglII to BamHI out of HIV"
     primer_bind     complement(1166..1189)
                     /note="pHAGE-A1050-0550"
     misc_feature    1245..1593
                     /note="RRE"
                     /note="Rev Responsive Element according to Belasco in 
                     Molecular Cell, Vol. 7, 603-614, March, 2001"
     misc_feature    1303..1536
                     /gene="rre"
                     /note="RRE"
                     /note="Rev Responsive Element"
     misc_feature    1357
                     /note="RRE Mutation"
                     /note="This G is a C in the WT HIV-1 genome"
     primer_bind     1391..1417
                     /note="pHAGE-S1501-2000"
     primer_bind     complement(1650..1678)
                     /note="pHAGE-A1550-1050"
     primer_bind     1898..1922
                     /note="pHAGE-S2001-2500"
     misc_feature    1911..1912
                     /note="SA 7925"
     misc_feature    2014..2027
                     /note="Junk"
                     /note="Linker Junk Sequence probably left over from 
                     cloning"
     misc_feature    2034..2211
                     /gene="cppt"
                     /note="HIV cpPu (Trip)"
                     /note="Central Poly Purine Track"
     primer_bind     complement(2084..2112)
                     /note="pHAGE-A2050-1550"
     misc_feature    2188..2189
                     /note="SA 4459"
     misc_feature    2218..3402
                     /note="Human EF1a Promoter"
     intron          2454..3045
     misc_feature    2531
                     /note="EF1a Mutation"
     misc_feature    3412..4713
                     /note="TAZ 4SA"
     CDS             3415..3480
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(4715..4739)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4745..5155
                     /note="UBCp"
     CDS             5163..5864
                     /codon_start=1
                     /note="TagBFP"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    5882..6473
                     /gene="WPRE"
                     /note="WPRE"
                     /note="Woodchuck Hepatitis Virus Posttranscriptional 
                     Regulatory Element"
     primer_bind     complement(5910..5944)
                     /note="pHAGE 3' CDS"
     primer_bind     complement(5988..6011)
                     /note="pHAGE-A3550-3050"
     primer_bind     6141..6161
                     /note="pHAGE-S4001-4500"
     misc_feature    6478..6579
                     /note="HIV nef"
     misc_feature    6528..6542
                     /note="HIV PPT"
                     /note="PolyPurine Tract"
     misc_feature    6545..6581
                     /note="dU3"
                     /note="1st Half of deleted U3"
     misc_binding    6601..6634
                     /note="loxp"
     misc_feature    6649..6667
                     /note="dU3"
                     /note="2nd Half of deleted U3"
     misc_feature    6668..6764
                     /note="HIV R"
     misc_feature    6765..6847
                     /note="HIV U5"
     primer_bind     6879..6901
                     /note="pHAGE-S6001-6500"
     promoter        7050..7084
                     /note="P-Bla"
                     /note="Beta Lactamase Promoter"
     CDS             7103..7942
                     /gene="AMPr"
                     /note="AMPr"
                     /note="Ampicillin Resistance Gene"
     primer_bind     complement(7152..7175)
                     /note="pHAGE-A6050-5550"
     primer_bind     7352..7376
                     /note="pHAGE-S6501-7000"
     primer_bind     complement(7621..7644)
                     /note="pHAGE-A6550-6050"
     primer_bind     7872..7896
                     /note="pHAGE-S7001-7500"
     primer_bind     complement(8132..8153)
                     /note="pHAGE-A7050-6550"
     rep_origin      8171..8704
                     /gene="ORI"
                     /note="ORI"
                     /note="Bacterial Origin of Replication"
     primer_bind     8376..8400
                     /note="pHAGE-S7501-8000"
     primer_bind     complement(8643..8664)
                     /note="pHAGE-A7550-7050"
     primer_bind     8879..8898
                     /note="pHAGE-S8001-8500"
     misc_feature    8941..9064
                     /gene="SV40 ori"
                     /note="SV40 ori"
     primer_bind     complement(9119..9145)
                     /note="pHAGE-A8050-7550"
     primer_bind     9384..9412
                     /note="pHAGE-S1-500"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc catggactac
     3421 aaagaccatg acggtgatta taaagatcat gacatcgatt acaaggatga cgatgacaag
     3481 cttacaagtt tgtacaaaaa agttgccacc atgaatccgg cctcggcgcc ccctccgctc
     3541 ccgccgcctg ggcagcaagt gatccacgtc acgcaggacc tagacacaga cctcgaagcc
     3601 ctcttcaact ctgtcatgaa tccgaagcct agctcgtggc ggaagaagat cctgccggag
     3661 tctttcttta aggagcctga ttcgggctcg cactcgcgcc agtccgccac cgactcgtcg
     3721 ggcggccacc cggggcctcg actggctggg ggtgcccagc atgtccgctc gcacgcgtcg
     3781 cccgcgtccc tgcagctggg caccggcgcg ggtgctgcgg gtagccccgc gcagcagcac
     3841 gcgcacctcc gccagcaggc ctacgacgtg accgacgagc tgccactgcc cccgggctgg
     3901 gagatgacct tcacggccac tggccagagg tacttcctca atcacataga aaaaatcacc
     3961 acatggcaag accctaggaa ggcgatgaat cagcctctga atcatatgaa cctccaccct
     4021 gccgtcagtt ccacaccagt gcctcagagg tccatggcag tatcccagcc aaatctcgtg
     4081 atgaatcacc aacaccagca gcagatggcc cccagtaccc tgagccagca gaaccacccc
     4141 actcagaacc cacccgcagg gctcatgagt atgcccaatg cgctgaccac tcagcagcag
     4201 cagcagcaga aactgcggct tcagagaatc cagatggaga gagaaaggat tcgaatgcgc
     4261 caagaggagc tcatgaggca ggaagctgcc ctctgtcgac agctccccat ggaagctgag
     4321 actcttgccc cagttcaggc tgctgtcaac ccacccacga tgaccccaga catgagatcc
     4381 atcactaata atagctcaga tcctttcctc aatggagggc catatcattc gagggagcag
     4441 gccactgaca gtggcctggg gttagggtgc tacagtgtcc ccacaactcc ggaggacttc
     4501 ctcagcaatg tggatgagat ggatacagga gaaaacgcag gacaaacacc catgaacatc
     4561 aatccccaac agacccgttt ccctgatttc cttgactgtc ttccaggaac aaacgttgac
     4621 ttaggaactt tggaatctga agacctgatc cccctcttca atgatgtaga gtctgctctg
     4681 aacaaaagtg agccctttct aacctggctg taatacccaa ctttcttgta caaagtggtg
     4741 tgaggatccg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg
     4801 cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc
     4861 tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat cagcagaagg
     4921 acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca gagagcggaa
     4981 caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg tggggcggtg
     5041 aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg
     5101 gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggcc gcccatatgg
     5161 ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg
     5221 acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga
     5281 ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta
     5341 ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct
     5401 tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg
     5461 gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca
     5521 agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct
     5581 gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca
     5641 tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat
     5701 ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg
     5761 aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca
     5821 gatactgcga cctccctagc aaactggggc acaagcttaa ttaaaggata tcgatagatc
     5881 ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5941 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6001 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6061 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6121 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6181 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6241 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6301 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6361 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6421 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggtacct
     6481 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6541 ggactggaag ggctaattca ctcccaacga agacaagatc acctgcagga caggcgcgcc
     6601 ataacttcgt atagcataca ttatacgaag ttatggtacc tgcgcgccct gctttttgct
     6661 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6721 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6781 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6841 tctagcaccc gggcgattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     6901 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6961 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7021 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7081 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7141 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7201 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7261 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7321 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7381 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7441 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7501 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7561 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7621 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7681 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7741 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7801 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7861 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7921 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7981 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8041 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8221 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8521 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8701 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8761 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8821 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8881 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8941 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9001 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     9061 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     9121 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9181 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     9241 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9301 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     9361 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     9421 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     9481 cttagtagt
//
