LOCUS       Exported                9401 bp DNA     circular SYN 07-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   TUDelft
  TITLE     Direct Submission
  JOURNAL   Exported Feb 7, 2024 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9401
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     terminator      6..52
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    53..246
                     /label=OriR194
     primer_bind     complement(310..326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    334..350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(358..388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    403..424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(712..1300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1471..2331)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2332..2436)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2910..2926
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2968..3161
                     /label=OriL191
     misc_feature    2968..2979
                     /label=minimal ORI
     misc_feature    3003..3025
                     /label=DBP binding site
     misc_feature    3168..3171
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        3189..3207
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             3239..3261
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3269..5752
                     /codon_start=1
                     /label=plsB
                     /translation="MTFCYPCRAFALLTRGFTSFMSGWPRIYYKLLNLPLSILVKSKSI
                     PADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVF
                     IHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPP
                     LRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQR
                     LAAVGPRLPARQDLFNKLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSY
                     EMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLY
                     HQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYS
                     VEYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKE
                     LRGATKEKESLPQMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVR
                     PAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLM
                     RNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAH
                     MLVLPSLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQ
                     RQGLITLQDDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESR
                     TVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELIT
                     SDVRLTIESATQGEG"
     terminator      5821..5868
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        5874..5892
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             5924..5946
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    5929..5937
                     /label=Epsilon
     misc_feature    5938..5944
                     /label=RBS
     CDS             5954..6688
                     /codon_start=1
                     /label=PlsC
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGRL
                     APLFGLKVECRKPTDAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIPFF
                     GQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFKTGA
                     FHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELAAHCR
                     SIMEQKIAELDKEVAEREAAGKV"
     misc_feature    6689..6691
                     /label=Stop Codon
     terminator      6760..6807
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        6813..6831
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6832..6856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6871..6893
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             6887..6892
                     /note="ribosome binding site"
     CDS             6901..6933
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /label=T7 tag
                     /translation="MASMTGGQQMG"
     CDS             6949..7806
                     /codon_start=1
                     /label=cdsA
                     /translation="LLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWGQ
                     LSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLLVL
                     FYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMILVWG
                     ADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPVTLLI
                     CSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFACLLLLV
                     FRTL"
     terminator      7856..7903
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        7909..7927
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7928..7952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7983..7988
                     /note="ribosome binding site"
     CDS             7997..9400
                     /codon_start=1
                     /label=pssA
                     /translation="MASMTGGQQMGRGSGCMLSKFKRNKHQQHLAQLPKISQSVDDVDF
                     FYAPADFRETLLEKIASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVD
                     WHRAQRGRIGAAASNTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDS
                     VLYSGASLNDVYLHQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRP
                     KSPEIKNDIRLFRQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQ
                     KLTICTPYFNLPAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLY
                     EINLRRFLSRLQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLD
                     LENAILIHDPQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIR
                     IDRLISRIL"
     CDS             7997..8029
                     /codon_start=1
                     /product="epitope tag from a T7 major capsid protein"
                     /label=T7 tag
                     /translation="MASMTGGQQMG"
ORIGIN
        1 tcgagtagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgcctcctat
       61 gattggttgt cttattacct tacttctatt atagtataac atgttaaacg atagtttgtc
      121 tacccttttc gacaaattga tgataataaa tagtataggt atatagtcgt gatttagttg
      181 ttagattctt gtcgaagata gtcggtcaat ggggaaatgg tgtatgttgt cgctgtaccc
      241 tactttattg gatcggatcc cgggcccgtc gactgcagag gcctgcatgc aagctttggc
      301 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
      361 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      421 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      481 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      541 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      601 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      661 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      721 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      781 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      841 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      901 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      961 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1021 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1081 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1141 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1201 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1261 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1321 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1381 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1441 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1501 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1561 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1621 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1681 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1741 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1801 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1861 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1921 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1981 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2041 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2101 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     2161 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2221 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     2281 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2341 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2401 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2461 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     2521 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     2581 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     2641 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     2701 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     2761 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     2821 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     2881 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg
     2941 tacctcgcga atgcatctag atccaataaa gtaagccccc accctcacat gataccattc
     3001 tcctaatatc gacataatcc gtcgatcctc ggcataccat gatcagggag ggaaactact
     3061 acttaatata tcaatctata gacctactag ataggtttgt caatgaacaa cataaaacga
     3121 cacagaatcc cacgttttag cgcttcgtct gtgtcgcatg tccatggcta gaacgtctca
     3181 atctctatta atacgactca ctatagggtc accacaacgg tttccctcta gaaataattt
     3241 tgtttaactt taagaaggag atatacatat gactttctgc tatccttgcc gcgcatttgc
     3301 attattaacc agaggcttta catcgtttat gtccggctgg ccacgaattt actacaaatt
     3361 actgaattta ccattaagca tcctggtaaa aagcaagtct attccggcag atcctgcccc
     3421 ggaactgggg ctggatacct ctcgtccaat tatgtacgtt ttaccgtaca actcgaaagc
     3481 agatttgctg acgttacgcg cccagtgtct ggctcatgac ttgcctgacc cgttagagcc
     3541 gctggaaatc gacggcacgc tactgccgcg ctatgtgttc attcacggcg ggccgcgtgt
     3601 attcacctat tacacgccga aagaagagtc tattaagctg ttccacgact atctcgattt
     3661 gcaccgtagc aacccaaatc tggatgtgca gatggtgcca gtatcggtga tgtttggtcg
     3721 cgcgccgggg cgtgaaaaag gcgaagtaaa cccgccgctg cgtatgctta acggcgtaca
     3781 gaaatttttc gccgtactgt ggctcggtcg cgacagtttt gtgcgtttct cgccgtcagt
     3841 ttcgctgcgc cgtatggcgg atgaacacgg cacggataaa actatcgcac agaaactggc
     3901 gcgcgtggcg cgtatgcact ttgcccgtca acgtctggct gctgtgggcc cacgtctgcc
     3961 tgctcgtcag gatctgttta ataagctgct cgcctcccgc gccattgcca aagcggtaga
     4021 agatgaagcg cgcagcaaaa aaatctccca tgaaaaagcg cagcagaacg cgattgcgct
     4081 gatggaagag attgcggcga atttctctta cgagatgatt cgcctgactg accgtattct
     4141 gggcttcacc tggaaccgcc tttaccaggg catcaacgtc cataacgccg agcgcgttcg
     4201 ccagctggcc cacgacggtc atgagctggt atatgtgcct tgccaccgca gccacatgga
     4261 ctacctgctg ctttcttacg tgctgtatca ccaggggctg gtgccgccgc atatcgccgc
     4321 tgggatcaac ctgaacttct ggccggccgg gccgattttc cgccgtctgg gggcgttctt
     4381 tattcgccgt acgttcaaag gcaataaact ttattccacc gttttccgtg agtatctcgg
     4441 cgaactgttc agccgtggtt attccgtcga gtacttcgtg gaaggcggtc gttcccgcac
     4501 ggggcgtttg ctggatccga aaaccggtac gctgtcgatg accattcagg cgatgctgcg
     4561 tggcggcacg cgtccgatta cgctgattcc gatctacatc ggttatgagc acgtcatgga
     4621 agtgggtact tacgccaaag aactgcgcgg cgcgacgaaa gagaaagaga gcctgccgca
     4681 gatgctgcgc ggtttaagca agctgcgtaa tctcggtcag ggttacgtca acttcggtga
     4741 accaatgccg ttgatgacct accttaacca gcacgtacca gactggcgtg aatctatcga
     4801 tcccatcgaa gcggtgcgtc cggcatggtt aacgccgacg gtcaataata ttgctgccga
     4861 tctgatggta cgcattaaca acgcaggcgc ggcaaacgcc atgaacctgt gctgtactgc
     4921 gcttctggcg tcacgtcagc gctcactcac ccgcgagcag ttaaccgagc aactcaactg
     4981 ctacctggat ctgatgcgca acgtacctta ctccacggac tctaccgttc cttcagccag
     5041 cgccagcgag cttatcgatc acgcgctgca aatgaacaag tttgaagtcg agaaagacac
     5101 aatcggcgac atcatcattc tgccgcgcga gcaagcggtg ctgatgacct actatcgcaa
     5161 caacattgcg catatgttgg tgctgccttc gctgatggcg gcaatcgtca cccagcatcg
     5221 ccacatctcc cgcgacgtat tgatggagca cgtcaatgtg ctttacccaa tgctgaaagc
     5281 ggagctgttc ctgcgctggg atcgcgacga gttgccggac gttattgatg cgctggcaaa
     5341 tgagatgcaa cgtcaggggc tgattaccct gcaagatgat gagttgcata tcaacccggc
     5401 gcattctcgc acgctacagc tgctggccgc aggcgcgcgc gaaacgctgc aacgttatgc
     5461 catcaccttc tggttgttga gtgccaaccc gtcgatcaac cgcggtacgc tggagaaaga
     5521 gagccgcacc gtcgcgcaac gtctctccgt gctgcacggc atcaacgcgc cggagttctt
     5581 cgacaaggcg gtgttcagtt ctctggtgct gacactgcgt gatgaagggt atatcagcga
     5641 tagcggcgat gccgaaccgg cagaaacgat gaaggtttat cagttgctgg cggagttgat
     5701 tacatcagac gtgcgtttga cgattgagag tgcgacgcag ggcgaagggt aagcttggat
     5761 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     5821 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgcg caataatacg
     5881 actcactata gggtcaccac aacggtttcc ctctagaaat aattttgttt aactttaaga
     5941 aggagatata catatgctat atatctttcg tcttattatt accgtgattt acagcatctt
     6001 agtctgtgta ttcggctcca tttactgcct tttcagcccg cgtaacccga aacatgtggc
     6061 cacctttggg catatgtttg gccgtcttgc gccgctgttt ggcctgaaag ttgagtgccg
     6121 taaacctaca gacgctgaaa gctacggcaa tgctatctat atcgctaacc accagaacaa
     6181 ctatgacatg gtgacagcat cgaacatcgt gcaaccgccg acggtgacgg taggtaaaaa
     6241 gagcttgctg tggatcccct tcttcgggca gttgtactgg ttaaccggca acttattgat
     6301 cgacagaaac aatcgcacta aagctcacgg caccattgcg gaagtagtga atcacttcaa
     6361 aaaacgccgt atttccatct ggatgttccc ggaaggaacc cgcagccgtg gtcgcggcct
     6421 gctaccgttc aagactggag catttcacgc ggcaattgcg gcgggcgtcc cgattattcc
     6481 cgtgtgcgtc tctacaactt cgaataagat taatcttaat cgactgcaca acggtctggt
     6541 gattgtcgaa atgctgccgc caattgacgt cagtcagtat ggcaaagatc aggttcgtga
     6601 gctggctgcc cattgtcgtt cgataatgga acaaaaaatc gccgagctcg ataaagaagt
     6661 cgcagaacgc gaagccgccg gaaaagttta agcttggatc cggctgctaa caaagcccga
     6721 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     6781 tctaaacggg tcttgagggg ttttttgcac tataatacga ctcactatag gggaattgtg
     6841 agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat
     6901 atggctagca tgactggtgg acagcaaatg ggtcgcggat ccggctgctt gctgaagtat
     6961 cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg
     7021 ccggtggggt tcgccattgt aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga
     7081 cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta
     7141 ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca tcaaccgctg
     7201 gttgaaatct cactttgggc ttcgctgggt tggtggattg tcgcgctatt gctggtgctg
     7261 ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct tatttttggc
     7321 gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg gcactatgac
     7381 gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt atggggcgct
     7441 gactccggcg catatatgtt tggcaaattg tttggtaaac ataagctggc accgaaggtt
     7501 tctccgggta aaacctggca aggctttatc ggtggactcg ctactgcagc ggtaatctca
     7561 tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact catttgctct
     7621 attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt taagcgtgaa
     7681 gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt agatcgtatt
     7741 gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt attcaggacg
     7801 ctttaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc
     7861 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgcttacta atacgactca
     7921 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta
     7981 agaaggagat atacatatgg ctagcatgac tggtggacag caaatgggtc gcggatccgg
     8041 ctgcatgttg tcaaaattta agcgtaataa acatcaacaa caccttgccc aactacccaa
     8101 gatttctcaa tcagttgatg atgtcgattt cttttacgct cccgccgact tccgggagac
     8161 gctgctggaa aaaatagcca gcgcgaagca gcgcatttgc attgtcgccc tgtatctcga
     8221 acaggatgac ggtggcaaag gcattctgaa cgcgttgtat gaggctaaaa ggcagcgtcc
     8281 ggaactggat gtgcgggtgc tggtcgactg gcatcgtgca caacgtggac gcattggcgc
     8341 tgcggcatct aacactaacg ctgactggta ctgccgcatg gcgcaggaaa atccgggcgt
     8401 agatgttccg gtttatggcg ttccaatcaa tactcgtgaa gcccttggtg ttctgcactt
     8461 taaaggcttt atcatcgacg atagcgtact ttatagcggt gccagcctga acgatgttta
     8521 cctgcatcag cacgataaat atcgctacga ccgttatcat ctgatccgta accgtaagat
     8581 gtcagacatt atgtttgaat gggttacaca gaatattatg aatggccgcg gcgttaatcg
     8641 tctggatgat gttaatcggc caaaaagccc ggaaatcaag aacgatattc gtctgttccg
     8701 ccaggagctg cgtgatgccg cttatcattt ccagggcgat gccgacaacg atcagctttc
     8761 tgtaacgccg ctagtggggc tggggaaatc gagtctgttg aacaagacca ttttccatct
     8821 tatgccttgt gccgagcaga aactaaccat ctgtacgcca tacttcaacc tgccagcaat
     8881 ccttgtgcgc aatattatcc agttgctgcg cgaagggaaa aaggtcgaaa ttattgttgg
     8941 tgataaaacc gcgaatgact tctacattcc ggaagatgaa cctttcaaga taattggcgc
     9001 attgccttat ctctatgaga tcaatctgcg tcgtttcctg agccgtttgc agtattacgt
     9061 caatactgac cagctagtgg ttcggttatg gaaagatgac gacaacacct atcacctgaa
     9121 agggatgtgg gttgatgata agtggatgtt gatcaccggt aataacctga acccgcgcgc
     9181 ctggcgtctg gatctggaaa acgccatttt gatccacgat ccgcaacttg agctggcgcc
     9241 acagcgagag aaagaactgg agctgatccg cgagcatacc accatcgtta agcactatcg
     9301 cgatctgcaa agtattgccg attatccggt gaaggttcgt aaactcatcc gccgtttgcg
     9361 ccgtatccgc atcgaccgat taattagccg catcctgtaa c
//
