LOCUS       pcDNA3.1-HaloCKAR2.2-T2A-EGFP        7646 bp ds-DNA     circular SYN 02-FEB-2024
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    pcDNA3.1(+)-ExRai-AKAR.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Mehta S, Zhang Y, Roth RH, Zhang JF, Mo A, Tenner B, Huganir RL and
            Zhang J
  TITLE     Single-fluorophore biosensors for sensitive and multiplexed
            detection of signalling activities.
  JOURNAL   Nat Cell Biol. 2018 Sep 24. pii: 10.1038/s41556-018-0200-6. doi:
            10.1038/s41556-018-0200-6.
  PUBMED    30250062
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     primer_bind     complement(44..63)
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /label="pRS-marker"
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /label="CMV promoter"
     primer_bind     769..789
                     /note="Human CMV immediate early promoter, forward primer"
                     /label="CMV-F"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     863..882
                     /note="T7 promoter, forward primer"
                     /label="T7"
     CDS             929..934
                     /created_by="Michelle"
                     /modified_by="Michelle"
                     /label="kozak"
     CDS             935..979
                     /created_by="Michelle"
                     /modified_by="Michelle"
                     /label="PKC substrate"
     CDS             980..>1006
                     /created_by="Michelle"
                     /modified_by="Michelle"
                     /label="Linker"
     misc_feature    <1007..>1015
                     /Source="referenceToDocument: urn:local:.:1bx-etqcyxv"
                     /label="cpHaloTag extraction_G (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     CDS             1007..1015
                     /created_by="Michelle"
                     /label="linker"
     CDS             1016..1948
                     /created_by="Michelle"
                     /label="cpHaloTag-V2"
     misc_feature    <1949..>1957
                     /Source="referenceToDocument: urn:local:.:1bx-etqcyxv"
                     /label="cpHaloTag extraction_G (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    <1949..>1957
                     /Source="referenceToDocument: urn:local:.:1bm-etqcyqx"
                     /label="cpHaloTag extraction_H (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     CDS             1949..1957
                     /created_by="Michelle"
                     /label="linker"
     CDS             1958..2380
                     /created_by="Michelle"
                     /label="FHA1"
     motif           2381..2395
                     /created_by="Michelle"
                     /modified_by="Michelle"
                     /label="LInker"
     CDS             2396..2449
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding
                     separatepolypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             2450..3163
                     /codon_start=1
                     /product="enhanced GFP"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     primer_bind     complement(3237..3254)
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /label="BGH-rev"
     polyA_signal    3243..3467
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3513..3941
                     /direction="RIGHT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     complement(3600..3619)
                     /note="F1 origin, reverse primer"
                     /label="F1ori-R"
     primer_bind     3810..3831
                     /note="F1 origin, forward primer"
                     /label="F1ori-F"
     primer_bind     complement(3950..3970)
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /label="pBABE 3'"
     promoter        3955..4284
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     rep_origin      4135..4270
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     4197..4216
                     /note="SV40 promoter/origin, forward primer"
                     /label="SV40pro-F"
     CDS             4351..5145
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418
                      (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /label="NeoR/KanR"
     primer_bind     complement(4405..4424)
                     /note="Neomycin resistance gene, reverse primer"
                     /label="Neo-R"
     primer_bind     5015..5034
                     /note="Neomycin resistance gene, forward primer"
                     /label="Neo-F"
     polyA_signal    5319..5440
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5356..5375)
                     /note="SV40 polyA, reverse primer"
                     /label="SV40pA-R"
     primer_bind     5410..5429
                     /note="SV40 polyA terminator, reverse primer"
                     /label="EBV-rev"
     primer_bind     complement(5489..5505)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /label="M13 rev"
     primer_bind     complement(5489..5505)
                     /note="In lacZ gene. Also called M13-rev"
                     /label="M13 Reverse"
     primer_bind     complement(5502..5524)
                     /note="In lacZ gene"
                     /label="M13/pUC Reverse"
     protein_bind    5513..5529
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5537..5567)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5582..5603
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     primer_bind     complement(5720..5737)
                     /note="L4440 vector, forward primer"
                     /label="L4440"
     rep_origin      complement(5891..6479)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(5971..5990)
                     /note="pBR322 origin, forward primer"
                     /label="pBR322ori-F"
     CDS             complement(6650..7510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     primer_bind     6671..6691
                     /Sequence="GGCACCTATCTCAGCGATCTG"
                     /Tm="60.3"
                     /%GC="57.1"
                     /Hairpin_Tm="41.7"
                     /Self_Dimer_Tm="None"
                     /created_by="Michelle"
                     /label="AMP_MFpr20"
     primer_bind     6747..6772
                     /Sequence="CGGGAGGGCTTACCATCTGGCCCCAG"
                     /Tm="72.0"
                     /%GC="69.2"
                     /Hairpin_Tm="58.8"
                     /Self_Dimer_Tm="16.9"
                     /created_by="Michelle"
                     /label="MFpr224"
     primer_bind     complement(6747..6772)
                     /Sequence="CTGGGGCCAGATGGTAAGCCCTCCCG"
                     /Tm="72.0"
                     /%GC="69.2"
                     /Hairpin_Tm="58.8"
                     /Self_Dimer_Tm="17.8"
                     /created_by="Michelle"
                     /label="MFpr225"
     primer_bind     6748..6767
                     /Sequence="GGGAGGGCTTACCATCTGGC"
                     /Tm="62.3"
                     /%GC="65.0"
                     /Hairpin_Tm="46.3"
                     /Self_Dimer_Tm="None"
                     /created_by="Michelle"
                     /label="MFpr350"
     primer_bind     complement(6749..6769)
                     /Sequence="GGGCCAGATGGTAAGCCCTCC"
                     /Tm="64.6"
                     /%GC="66.7"
                     /Hairpin_Tm="57.2"
                     /Self_Dimer_Tm="11.7"
                     /created_by="Michelle"
                     /label="Mfpr0211"
     primer_bind     7273..7292
                     /note="Ampicillin resistance gene, reverse primer"
                     /label="Amp-R"
     promoter        complement(7511..7615)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcgg ccgccaccgg atccatgcga ttcagaagat tccagacgtt
      961 gaaggacaag gctaaggctg gcggcaccgg cggcagcgag ctcagctggc ggcgggagac
     1021 cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt
     1081 tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg agatggacca
     1141 ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct tcccaaacga
     1201 gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat acatggactg
     1261 gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg ttctgatccc
     1321 accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg tggacatcgg
     1381 cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg agatcgcgcg
     1441 ctggctgtcg acgctcgaga tttccggcga gccaaccact ggaggcagcg gaggcacagg
     1501 aggcagcgga ggcacaggag gcagcatggc agaaatcggt actggctttc cattcgaccc
     1561 ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg
     1621 cacccctgtg ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc gcaacatcat
     1681 cccgcatgtt gcaccgaccc atcgctgcat tgctccagac ctgatcggta tgggcaaatc
     1741 cgacaaacca gacctgggtt atttcttcga cgaccacgtc cgcttcatgg atgccttcat
     1801 cgaagccctg ggtctggaag aggtcgtcct ggtcattcac gactggggct ccgctctggg
     1861 tttccactgg gccaagcgca atccagagcg cgtcaaaggt attgcattta tggagttcat
     1921 ccgccctatc ccgacctggg acgaatggac catcagcaag ttttctcaag aacagatcgg
     1981 cgaaaacatt gtgtgcaggg tcatttgtac cacgggtcaa attcccatcc gagatttgtc
     2041 agctgatatt tcacaagtgc ttaaggaaaa acgatccata aagaaagttt ggacatttgg
     2101 tagaaaccca gcctgtgact atcatttagg aaacatttca agactgtcaa ataagcattt
     2161 ccaaatacta ctaggagaag acggtaacct tttattgaat gacatttcca ctaatgggac
     2221 ctggttaaat gggcaaaaag tcgagaagaa cagcaatcag ttactgtctc aaggtgatga
     2281 aataaccgtt ggtgtaggcg tggaatcaga tattttatct ctggtcattt tcataaacga
     2341 caaatttaag cagtgcctcg agcagaacaa agttgatcgc ggccgcggca gtggagaggg
     2401 cagaggaagt ctgctaacat gcggtgacgt cgaggagaat cctggcccag tgagcaaggg
     2461 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2521 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2581 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     2641 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     2701 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2761 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2821 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2881 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2941 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     3001 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     3061 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3121 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagaat tctgcagata
     3181 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     3241 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3301 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3361 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3421 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     3481 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     3541 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3601 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3661 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3721 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3781 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3841 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3901 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     3961 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4021 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4081 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4141 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4201 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     4261 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     4321 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     4381 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     4441 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     4501 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     4561 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     4621 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     4681 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     4741 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     4801 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     4861 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     4921 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     4981 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     5041 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     5101 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     5161 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     5221 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5281 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     5341 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5401 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     5461 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5521 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5581 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5641 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5701 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5761 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5821 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5881 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5941 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     6001 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     6061 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6121 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6181 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6241 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6301 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6361 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6421 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6481 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6541 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6601 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6661 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6721 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6781 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6841 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6901 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6961 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7021 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7081 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7141 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7201 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7261 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7321 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7381 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7441 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7501 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7561 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7621 ttccccgaaa agtgccacct gacgtc
//
