LOCUS       pcDNA3_Key-14L-TurboID7 6449 bp ds-DNA     circular     30-SEP-2022
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pcDNA3-AktAR.gb
FEATURES             Location/Qualifiers
     misc_feature    232..819
                     /label="CMV prom"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     primer_bind     863..882
                     /label="T7"
                     /ApEinfo_revcolor="#008b8b"
                     /ApEinfo_fwdcolor="#008b8b"
     misc_feature    877..896
                     /label="JZZ_96"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     Primer          complement(877..896)
                     /label="JZZ_60"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    877..1274
                     /label="JZZ_93"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    897..902
                     /label="kozak"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             903..2018
                     /label="Translation 903-2018"
                     /translation="MGSSHHHHHHSSEQKLISEEDLGGSEQKLISEEDLGGSEQKLISEEDLGGSMDPDEARKAIARVKRESKRIVEDAERLSREAAAASEKSSREAERLSREAAAASEKISREGGSGGTTGSGGGSGGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK*"
     misc_feature    915..932
                     /label="His tag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    939..1055
                     /label="3x Myc Tag"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1056..1232
                     /label="Key"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1056..2015
                     /label="Key protein (essential)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1151..1170
                     /label="JZZ_107"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1255..1292
                     /label="JZZ_98"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1259..1274)
                     /label="JZZ_97"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1275..2015
                     /label="TurboID(74-C)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(1995..2038)
                     /label="JZZ_95"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2019..2059
                     /label="JZZ_109"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     terminator      2042..2269
                     /label="bGH PA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2938..3015
                     /label="SV40 ORI"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             3157..3948
                     /label="Kan/neoR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4204..4234
                     /label="JZZ_62"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(4204..4234)
                     /label="JZZ_63"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4288..4308)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#008b8b"
                     /ApEinfo_fwdcolor="#008b8b"
     misc_binding    complement(4314..4336)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Promoter        complement(4341..4370)
                     /label="lac"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      complement(4676..5304)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             complement(5456..6115)
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     Promoter        complement(6355..6383)
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCgcca
      901 ccATGGGGTC TAGCCATCAC CATCATCACC ATTCTAGCGA GCAGAAACTC ATCTCTGAAG
      961 AGGATCTCGG TGGTAGTGAA CAAAAGTTGA TATCCGAGGA GGATTTGGGT GGGAGCGAGC
     1021 AGAAACTTAT CAGTGAAGAA GATTTGGGGG GCTCCATGGA TCCTGACGAG GCAAGGAAAG
     1081 CCATAGCGAG GGTTAAAAGG GAAAGCAAAC GAATAGTAGA AGACGCGGAA AGGCTTagtC
     1141 GAGAAGCAGC GGCGGCAAGT GAGAAAtccT CCCGAGAGGC GGAGAGGCTC tccCGGGAGG
     1201 CCGCTGCTGC GTCCGAGAAA ATTTCCCGGG AGGGCGGTAG TGGTGGAACC ACCGGGAGCG
     1261 GTGGTGGTTC CGGAGGACAG CTGGATGGGG GTTCCGTAGC TGTGTTGCCG GTTGTCGACA
     1321 GTACAAACCA GTACCTTCTG GATCGGATCG GTGAGCTGAA GTCCGGCGAC GCCTGCATAG
     1381 CGGAGTATCA GCAAGCGGGG AGGGGTAGCA GAGGGAGAAA GTGGTTCTCC CCTTTTGGGG
     1441 CCAACCTCTA CCTTTCAATG TTTTGGCGAC TCAAAAGGGG ACCCGCAGCA ATCGGACTGG
     1501 GCCCGGTGAT TGGCATCGTG ATGGCCGAGG CACTGCGGAA ATTGGGGGCG GACAAGGTGC
     1561 GCGTAAAGTG GCCGAACGAC TTGTATCTCC AAGACAGAAA ACTCGCTGGT ATCCTCGTTG
     1621 AACTGGCAGG TATCACAGGA GACGCAGCAC AAATCGTAAT CGGAGCTGGA ATCAATGTAG
     1681 CAATGAGACG CGTGGAAGAG AGTGTTGTCA ATCAAGGTTG GATTACTCTG CAGGAAGCAG
     1741 GTATCAATCT CGATCGGAAC ACGCTGGCCG CGACTCTCAT TAGAGAGCTG CGAGCGGCAC
     1801 TGGAGTTGTT CGAACAAGAG GGACTTGCGC CCTACCTTCC CCGATGGGAG AAACTGGATA
     1861 ACTTCATAAA TAGGCCCGTT AAGTTGATAA TTGGCGATAA AGAGATTTTC GGAATTAGTA
     1921 GGGGTATCGA CAAACAAGGT GCCTTGCTCC TTGAGCAAGA CGGCGTGATA AAGCCCTGGA
     1981 TGGGTGGTGA AATCAGTCTC CGAAGTGCAG AGAAAtagGA ATTCTGCAGA TATCCATCcc
     2041 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     2101 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     2161 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     2221 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     2281 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     2341 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2401 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2461 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2521 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2581 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2641 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     2701 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     2761 gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg
     2821 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     2881 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     2941 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3001 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     3061 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     3121 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     3181 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     3241 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     3301 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     3361 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     3421 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     3481 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     3541 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     3601 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     3661 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     3721 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     3781 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     3841 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     3901 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     3961 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     4021 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     4081 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     4141 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     4201 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     4261 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4321 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4381 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     4441 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4501 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4861 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     4921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5101 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     5161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5461 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5521 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5581 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5641 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5701 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5761 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5821 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5881 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5941 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6001 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6061 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6121 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6181 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6241 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6301 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6361 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6421 catttccccg aaaagtgcca cctgacgtc
//