LOCUS       pcDNA3_Lyn-D73          6479 bp ds-DNA     circular     04-NOV-2021
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pcDNA3-AktAR.gb
FEATURES             Location/Qualifiers
     misc_feature    232..819
                     /label="CMV prom"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     primer_bind     863..882
                     /label="T7"
                     /ApEinfo_revcolor="#008b8b"
                     /ApEinfo_fwdcolor="#008b8b"
     misc_feature    877..896
                     /label="JZZ_96"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     Primer          complement(877..896)
                     /label="JZZ_60"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    877..1304
                     /label="JZZ_93"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    897..902
                     /label="kozak"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             903..2048
                     /label="Translation 903-2048"
                     /translation="MGCIKSKRKDKGSSHHHHHHSSEQKLISEEDLGGSEQKLISEEDLGGSEQKLISEEDLGGSMDPDEARKAIARVKRESKRIVEDAERLSREAAAASEKSSREAERLSREAAAASEKISREGGSGGTTGSGGGSGGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK*"
     misc_feature    906..935
                     /label="Lyn"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    945..962
                     /label="His tag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    969..1085
                     /label="3x Myc Tag"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1086..1262
                     /label="Key"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1181..1200
                     /label="JZZ_107"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1285..1322
                     /label="JZZ_98"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1289..1304)
                     /label="JZZ_97"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1305..2045
                     /label="TurboID(74-C)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2025..2068)
                     /label="JZZ_95"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2049..2089
                     /label="JZZ_109"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     terminator      2072..2299
                     /label="bGH PA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2968..3045
                     /label="SV40 ORI"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             3187..3978
                     /label="Kan/neoR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4234..4264
                     /label="JZZ_62"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(4234..4264)
                     /label="JZZ_63"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4318..4338)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#008b8b"
                     /ApEinfo_fwdcolor="#008b8b"
     misc_binding    complement(4344..4366)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Promoter        complement(4371..4400)
                     /label="lac"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      complement(4706..5334)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             complement(5486..6145)
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     Promoter        complement(6385..6413)
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCgcca
      901 ccATGGGCTG CATCAAGAGC AAGAGGAAGG ACAAAGGGTC TAGCCATCAC CATCATCACC
      961 ATTCTAGCGA GCAGAAACTC ATCTCTGAAG AGGATCTCGG TGGTAGTGAA CAAAAGTTGA
     1021 TATCCGAGGA GGATTTGGGT GGGAGCGAGC AGAAACTTAT CAGTGAAGAA GATTTGGGGG
     1081 GCTCCATGGA TCCTGACGAG GCAAGGAAAG CCATAGCGAG GGTTAAAAGG GAAAGCAAAC
     1141 GAATAGTAGA AGACGCGGAA AGGCTTagtC GAGAAGCAGC GGCGGCAAGT GAGAAAtccT
     1201 CCCGAGAGGC GGAGAGGCTC tccCGGGAGG CCGCTGCTGC GTCCGAGAAA ATTTCCCGGG
     1261 AGGGCGGTAG TGGTGGAACC ACCGGGAGCG GTGGTGGTTC CGGAGGACAG CTGGATGGGG
     1321 GTTCCGTAGC TGTGTTGCCG GTTGTCGACA GTACAAACCA GTACCTTCTG GATCGGATCG
     1381 GTGAGCTGAA GTCCGGCGAC GCCTGCATAG CGGAGTATCA GCAAGCGGGG AGGGGTAGCA
     1441 GAGGGAGAAA GTGGTTCTCC CCTTTTGGGG CCAACCTCTA CCTTTCAATG TTTTGGCGAC
     1501 TCAAAAGGGG ACCCGCAGCA ATCGGACTGG GCCCGGTGAT TGGCATCGTG ATGGCCGAGG
     1561 CACTGCGGAA ATTGGGGGCG GACAAGGTGC GCGTAAAGTG GCCGAACGAC TTGTATCTCC
     1621 AAGACAGAAA ACTCGCTGGT ATCCTCGTTG AACTGGCAGG TATCACAGGA GACGCAGCAC
     1681 AAATCGTAAT CGGAGCTGGA ATCAATGTAG CAATGAGACG CGTGGAAGAG AGTGTTGTCA
     1741 ATCAAGGTTG GATTACTCTG CAGGAAGCAG GTATCAATCT CGATCGGAAC ACGCTGGCCG
     1801 CGACTCTCAT TAGAGAGCTG CGAGCGGCAC TGGAGTTGTT CGAACAAGAG GGACTTGCGC
     1861 CCTACCTTCC CCGATGGGAG AAACTGGATA ACTTCATAAA TAGGCCCGTT AAGTTGATAA
     1921 TTGGCGATAA AGAGATTTTC GGAATTAGTA GGGGTATCGA CAAACAAGGT GCCTTGCTCC
     1981 TTGAGCAAGA CGGCGTGATA AAGCCCTGGA TGGGTGGTGA AATCAGTCTC CGAAGTGCAG
     2041 AGAAAtagGA ATTCTGCAGA TATCCATCcc tcgactgtgc cttctagttg ccagccatct
     2101 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2161 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2221 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2281 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     2341 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     2401 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2461 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg gcatcccttt agggttccga
     2521 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2581 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2641 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2701 ttataaggga ttttggggat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2761 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     2821 ccccaggcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     2881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     2941 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3001 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     3061 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3121 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     3181 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     3241 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3301 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     3361 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     3421 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     3481 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     3541 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     3601 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     3661 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     3721 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     3781 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     3841 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     3901 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     3961 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     4021 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4081 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4141 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4201 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4261 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     4321 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4381 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4441 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     4921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5161 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//