LOCUS       pcDNA3_CYP450-D73       6527 bp ds-DNA     circular     04-NOV-2021
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pcDNA3-AktAR.gb
FEATURES             Location/Qualifiers
     misc_feature    232..819
                     /label="CMV prom"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
     primer_bind     863..882
                     /label="T7"
                     /ApEinfo_revcolor="#008b8b"
                     /ApEinfo_fwdcolor="#008b8b"
     misc_feature    877..896
                     /label="JZZ_96"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     Primer          complement(877..896)
                     /label="JZZ_60"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    877..1352
                     /label="JZZ_93"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    897..902
                     /label="kozak"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             903..2096
                     /label="Translation 903-2096"
                     /translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGGSSHHHHHHSSEQKLISEEDLGGSEQKLISEEDLGGSEQKLISEEDLGGSMDPDEARKAIARVKRESKRIVEDAERLSREAAAASEKSSREAERLSREAAAASEKISREGGSGGTTGSGGGSGGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK*"
     misc_feature    906..983
                     /label="CYP450"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    993..1010
                     /label="His tag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1017..1133
                     /label="3x Myc Tag"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1134..1310
                     /label="Key"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1229..1248
                     /label="JZZ_107"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1333..1370
                     /label="JZZ_98"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1337..1352)
                     /label="JZZ_97"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1353..2093
                     /label="TurboID(74-C)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2073..2116)
                     /label="JZZ_95"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2097..2137
                     /label="JZZ_109"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     terminator      2120..2347
                     /label="bGH PA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3016..3093
                     /label="SV40 ORI"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             3235..4026
                     /label="Kan/neoR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    4282..4312
                     /label="JZZ_62"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(4282..4312)
                     /label="JZZ_63"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     complement(4366..4386)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#008b8b"
                     /ApEinfo_fwdcolor="#008b8b"
     misc_binding    complement(4392..4414)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     Promoter        complement(4419..4448)
                     /label="lac"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     rep_origin      complement(4754..5382)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
     CDS             complement(5534..6193)
                     /label="AmpR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     Promoter        complement(6433..6461)
                     /label="Amp prom"
                     /ApEinfo_revcolor="#4682b4"
                     /ApEinfo_fwdcolor="#4682b4"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCgcca
      901 ccATGGATCC CGTGGTGGTA TTGGGCTTGT GTCTCTCATG TCTGCTTCTC TTGTCATTGT
      961 GGAAACAATC CTACGGAGGT GGCGGGTCTA GCCATCACCA TCATCACCAT TCTAGCGAGC
     1021 AGAAACTCAT CTCTGAAGAG GATCTCGGTG GTAGTGAACA AAAGTTGATA TCCGAGGAGG
     1081 ATTTGGGTGG GAGCGAGCAG AAACTTATCA GTGAAGAAGA TTTGGGGGGC TCCATGGATC
     1141 CTGACGAGGC AAGGAAAGCC ATAGCGAGGG TTAAAAGGGA AAGCAAACGA ATAGTAGAAG
     1201 ACGCGGAAAG GCTTagtCGA GAAGCAGCGG CGGCAAGTGA GAAAtccTCC CGAGAGGCGG
     1261 AGAGGCTCtc cCGGGAGGCC GCTGCTGCGT CCGAGAAAAT TTCCCGGGAG GGCGGTAGTG
     1321 GTGGAACCAC CGGGAGCGGT GGTGGTTCCG GAGGACAGCT GGATGGGGGT TCCGTAGCTG
     1381 TGTTGCCGGT TGTCGACAGT ACAAACCAGT ACCTTCTGGA TCGGATCGGT GAGCTGAAGT
     1441 CCGGCGACGC CTGCATAGCG GAGTATCAGC AAGCGGGGAG GGGTAGCAGA GGGAGAAAGT
     1501 GGTTCTCCCC TTTTGGGGCC AACCTCTACC TTTCAATGTT TTGGCGACTC AAAAGGGGAC
     1561 CCGCAGCAAT CGGACTGGGC CCGGTGATTG GCATCGTGAT GGCCGAGGCA CTGCGGAAAT
     1621 TGGGGGCGGA CAAGGTGCGC GTAAAGTGGC CGAACGACTT GTATCTCCAA GACAGAAAAC
     1681 TCGCTGGTAT CCTCGTTGAA CTGGCAGGTA TCACAGGAGA CGCAGCACAA ATCGTAATCG
     1741 GAGCTGGAAT CAATGTAGCA ATGAGACGCG TGGAAGAGAG TGTTGTCAAT CAAGGTTGGA
     1801 TTACTCTGCA GGAAGCAGGT ATCAATCTCG ATCGGAACAC GCTGGCCGCG ACTCTCATTA
     1861 GAGAGCTGCG AGCGGCACTG GAGTTGTTCG AACAAGAGGG ACTTGCGCCC TACCTTCCCC
     1921 GATGGGAGAA ACTGGATAAC TTCATAAATA GGCCCGTTAA GTTGATAATT GGCGATAAAG
     1981 AGATTTTCGG AATTAGTAGG GGTATCGACA AACAAGGTGC CTTGCTCCTT GAGCAAGACG
     2041 GCGTGATAAA GCCCTGGATG GGTGGTGAAA TCAGTCTCCG AAGTGCAGAG AAAtagGAAT
     2101 TCTGCAGATA TCCATCcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2161 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2221 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2281 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2341 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2401 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2461 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2521 ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt tagtgcttta
     2581 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     2641 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     2701 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     2761 ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     2821 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc
     2881 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2941 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     3001 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3061 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     3121 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     3181 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     3241 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3301 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3361 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     3421 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3481 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3541 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3601 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3661 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3721 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     3781 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3841 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3901 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3961 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4021 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4081 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4141 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     4201 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4261 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4321 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     4381 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4441 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4501 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4561 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4621 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     4981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     5221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5401 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5581 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5821 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5941 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6121 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6481 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//