LOCUS       pCfB2188-PrARG8-        7757 bp DNA     circular SYN 22-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7757)
  AUTHORS   RNAcious laboratory
  TITLE     Direct Submission
  JOURNAL   Exported Feb 22, 2024 from SnapGene 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      12..201
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     protein_bind    249..282
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1734..1767
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(1811..1829)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1839..1855)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2448..2481)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(2951..2967)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(3237..3825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(3949..4740,4741..4809))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4810..4901)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    5194..5227
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(5837..6002)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        6041..7040
                     /label=ARG8 promtoer
     CDS             7041..7757
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKTDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 cgcgtgcatt catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
       61 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
      121 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
      181 aaggttttgg gacgctcgaa gatcgcgtca gctgaagctt cgtacgctgc aggtcgacaa
      241 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtcta gagatcccaa
      301 tacaacagat cacgtgatct ttttgtaaga tgaagttgaa gtagtgttgc accgtgccaa
      361 tgcaggtggc tattagatta aatatgtgat ttgttctatt aagtttcctg tataattaat
      421 ggggagcgct gattctcttt tggtacgctt cccatccagc atttctgtat ctttcacctt
      481 caaccttagg atctctaccc ttggcgaaaa gtcctctgcc aacaatgatg atatctgatc
      541 caccacttac aacttcgtcg acggttctgt actgctgacc caatgcatcg cctttgtcgt
      601 ctaaacctac acctggggtc atgattagcc aatcaaaccc ttcttctctt cctcccatat
      661 cgttctgagc aatgaaccca ataacgaaat ctttatcact ctttgcaata tcaacggtac
      721 ccttagtata ttcaccgtgt gctagagaac ccttggaaga taattcagca agcatcaata
      781 atccccttgg ttctttggtg acctcttgcg caccttgttt caagccagca acaataccag
      841 caccagtaac cccgtgggcg ttggtgatat cagaccattc tgcgatacgg taaacgcccg
      901 atgtatattg taatttgact gtgttaccga tatcggcgaa ttttctgtcc tcaaatatca
      961 agaacttgta tttctctgcc aatgctttca atggaacgac agtaccctca taactgaaat
     1021 catccaagat atcaacgtgt gttttcaaaa ggcaaatgta tggacccaac gtttcaacaa
     1081 gtttcaatag ctcatcagtc gaacgaacgt caagagaagc acacaaattg gttttctttt
     1141 catccattaa acgtaaaagt ttcgatgcaa ccggacttgc atgagtctca gctctactgg
     1201 tatatgattt tgtggacatg gtgcaactaa ttgacgggag tgtattgacg ctggcgtact
     1261 ggctttcaca aaatggccca atcacaacca catcttagat agttgaaatg actttagata
     1321 acatcaattg agatgagctt aatcatgtca aagctaaaag tgtcaccatg aacgacaatt
     1381 cttaagcaaa tcacgtgata tagatccacg aataaccacc atttgatgct cgaggcaagt
     1441 aatgtgtgta aaaaaatgcg ttaccaccat ccaatgcaga ccgatcttct acccagaatc
     1501 acatatattt atgtaccgag tacctttttt ctatcttcca attgcttctc ccatatgatt
     1561 gtctccgtaa gctcgaaatt tctaagttgg attttaatct tcacgcagga tgacagttcg
     1621 atgagcttct gaggagtgtt tagaacataa tcatttatcc atggtctatc tcttcttgtc
     1681 gctttttctc ctcgatagaa cctaaataaa acgagctctc gagaaccctt aatataactt
     1741 cgtataatgt atgctatacg aagttattag gtgatatcag atccactagt ggcctatgca
     1801 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     1861 cgtgactggg aaaaccctgg cgttacccct gcaggactag tgctgaggca ttaatcctgc
     1921 ataatcggcc tcacagaggg atcccgttac ccatctatgc tgaagattta tcatactatt
     1981 cctccgctcg tttctttttt cagtgaggtg tgtcgtgaaa gaaaacccac aattaaactt
     2041 tcaacaaccg ggcgactagg aagagagtag tgggcgcgga tgacgaaggc taaggtcact
     2101 tcttcgtttc ctttattggg gtttccgtgt agccttcccc tgaatagtgt gggacgtttt
     2161 atgagaagcc gtaagaaata ggcaaattga gttatgacaa gtagacatga tgccgcagcc
     2221 ttgcctgact ttacgtctcc ttcatgaata agtttttcta tcgagttctt ttcctttttt
     2281 cgccttaatt agctcaatta agcctgtcct cactactttt ctttttctta tcggctttgt
     2341 gccacaccta accttcgaat gctgttttat tccgttctta catgggatgg taatgccttg
     2401 gcgagattaa gacctcagcg cggccgcaaa tttaaataaa atgaagtgaa gttcctatac
     2461 tttctagaga ataggaactt ctatagtgag tcgaataagg gcgacacaaa atttattcta
     2521 aatgcataat aaatactgat aacatcttat agtttgtatt atattttgta ttatcgttga
     2581 catgtataat tttgatatca aaaactgatt ttccctttat tattttcgag atttattttc
     2641 ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa taatagatga
     2701 atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt aaagtgcgtt
     2761 gcttttttct catttataag gttaaataat tctcatatat caagcaaagt gacaggcgcc
     2821 cttaaatatt ctgacaaatg ctctttccct aaactccccc cataaaaaaa cccgccgaag
     2881 cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc ccagggggcc
     2941 cgagcttaag actggccgtc gttttacaac acagaaagag tttgtagaaa cgcaaaaagg
     3001 ccatccgtca ggggccttct gcttagtttg atgcctggca gttccctact ctcgccttcc
     3061 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtgggctaac
     3661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3841 ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt taagggattt
     3901 tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgctttt accaatgctt
     3961 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4021 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gcgctgcgat
     4081 gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc agccagccgg
     4141 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4201 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4261 cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4321 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4381 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4441 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4501 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4561 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4621 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4681 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4741 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4801 aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4861 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca gtgttacaac
     4921 caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg gctcataaca
     4981 ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag
     5041 aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga gtagggaact
     5101 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg cccgggctaa
     5161 ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta gaaagtatag
     5221 gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcgt ctatgaggag
     5281 actgttagtt ggatatcagt aatgagacga aaaagctcga aatgaatgga tatattcttt
     5341 ttgctactgg caactgttga atatttaatg ttaaaacaaa ctaactgagg tatattcgta
     5401 tctgtatgta cacatatact atatacagga aaagataagc aagagagagg atatcaacta
     5461 cgagagcgat cgattatata tcaaaagctg tccgctttgc cacccataat cggcgcttag
     5521 tttcggagtt caatcataat tctaccacct tacactcaac ttactcttta actcctatag
     5581 tataatatcg ccactgaccc catattaaaa aatttttttg ctcgatcttc tatcctcttt
     5641 aggttaattg tcgctgttat tgtctagatt ttttctcgga gatggcgcat ctatttgccg
     5701 tcaaaagatc ctctcatacc atattaagta aattgcctcc atttcttttt cctcgggcag
     5761 agaaactcgc aggcaacttg ctctcgaagt ggtcacgtat taagtcctca gcgagctcgc
     5821 atggaatgcg tgcgatgagc gacctcatgc tatacctgag aaagcaacct gacctacagg
     5881 aaagagttac tcaagaataa gaattttcgt tttaaaacct aagagtcact ttaaaatttg
     5941 tatacactta ttttttttat aacttattta ataataaaaa tcataaatca taagaaattc
     6001 gcttatttag aagtgtcaac aacgtatcta caacggaatg tcctgtagca agaatgtgtc
     6061 acacttttac aactatttct agaaataacc agctactgct tattggaggt agaaaggcac
     6121 cacaccaagg tctctctgat aattggatat ttgatatgaa aacaagagag tggtcgatga
     6181 tcaagagttt atcacacacg agattcagac atagtgcatg tagcttaccg gatggaaacg
     6241 tcctgatcct tggtggggtt acagaaggac ctgccatgct gctatacaat gtcaccgaag
     6301 aaatcttcaa agacgtaact ccaaaagatg aattcttcca aaattcgcta gtatcagcgg
     6361 gtctagaatt tgatccggtt tccaaacaag gcataatatt gggtggtgga tttatggatc
     6421 aaacaactgt ctcggacaag gcaattattt tcaaatatga tgcggaaaat gctactgaac
     6481 caattacggt gattaagaag ctccagcacc ccttatttca acgttatgga tcccagataa
     6541 aatacattac tccaagaaag ctattaatag tcggtggaac aagtccttcc gggctatttg
     6601 accggaccaa ctctataatc agccttgatc cgctgagtga gacgctaaca tcaattccca
     6661 tatcaagacg catatgggaa gatcactcac taatgcttgc tggatttagt ttggtgtcca
     6721 cttctatggg caccatacat atcattggag gcggtgccac ttgctacggc tttggatcag
     6781 tgactaatgt aggtcttaag ctcatagcaa tcgcgaaata atgctcctat cgggattcga
     6841 atggttgcca gctcgctatg tgactcactt aaagtacatg atgcgtcatt gtgcactttt
     6901 ttacgaaata atggcctttt tccgctctat ttaaaagcgt gaaaaaaaaa ttgcaacatg
     6961 aagaaaattc gactcttaag tgcaagtgac tgcgaacatt tttttcgttt gttagaataa
     7021 ttcaagaatc gctaccaatc atgtctaaag gtgaagaatt attcactggt gttgtcccaa
     7081 ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg
     7141 aaggtgatgc tacttacggt aaattgacct taaaatttat ttgtactact ggtaaattgc
     7201 cagttccatg gccaacctta gtcactactt tcggttatgg tgttcaatgt tttgcgagat
     7261 acccagatca tatgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc
     7321 aagaaagaac tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt
     7381 ttgaaggtga taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaagatg
     7441 gtaacatttt aggtcacaaa ttggaataca actataactc tcacaatgtt tacatcatgg
     7501 ctgacaaaca aaagaatggt atcaaagtta acttcaaaat tagacacaac attgaagatg
     7561 gttctgttca attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct
     7621 tgttaccaga caaccattac ttatccactc aatctgcctt atccaaagat ccaaacgaaa
     7681 agacagacca catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg
     7741 atgaattgta caaataa
//
