LOCUS       Exported                3110 bp ds-DNA    circular SYN 12-FEB-2024
DEFINITION  Universal Test Construct 7.0.
ACCESSION   pOP01
VERSION     .
KEYWORDS    pOP01
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 12, 2024 from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    16..429
                     /standard_name="5C2LF"
                     /note="5C2LF"
     misc_feature    complement(16..72)
                     /standard_name="L3S2P22"
                     /note="L3S2P22"
                     /note="Geneious type: terminator"
     misc_feature    73..372
                     /standard_name="Spacer002"
                     /note="Spacer002"
     misc_feature    373..429
                     /standard_name="L3S2P56"
                     /note="L3S2P56"
                     /note="Geneious type: terminator"
     misc_feature    434..451
                     /standard_name="T7 promoter"
                     /note="T7 promoter"
                     /note="Geneious type: promoter"
     5'UTR           456..515
                     /standard_name="gene10 5'UTR T7"
                     /note="gene10 5'UTR T7"
     CDS             517..1035
                     /standard_name="NanoLuc"
                     /note="NanoLuc"
     3'UTR           1036..1225
                     /standard_name="126kDa 3'UTR TMV"
                     /note="126kDa 3'UTR TMV"
     misc_feature    complement(1253..1349)
                     /standard_name="AmpR Terminator"
                     /note="AmpR Terminator"
                     /note="Geneious type: terminator"
     CDS             complement(1350..2207)
                     /standard_name="AmpR"
                     /note="AmpR"
     misc_feature    complement(2208..2338)
                     /standard_name="AmpR Promoter"
                     /note="AmpR Promoter"
                     /note="Geneious type: promoter"
     rep_origin      2415..3003
                     /direction=RIGHT
ORIGIN
        1 aacacgtctc gtactggacc aaaacgaaaa aaggccgctt tcgcggcctc ttttctggaa
       61 tttggtaccg agtcgacacc ctaccgtatc gctgtggcag gcacaggatc taaccggtcc
      121 tggaccggcg taagttgctg tacagccaaa tcttccgtac taattctaca ggatttgtca
      181 actgcaaacg aatttctagt cggaacggat cacgtgcacc ggggtatgcc gtggttgaat
      241 atcggcacat gaggggctcg tgggacactt ccagaactgt ctggcgtgcg aagtctctgc
      301 taaatgcaaa acagtcagga ctcgttacag ctcgtgtatg ggagctccgg gtaggccgat
      361 cgtcaattgc ggctcggtac caaattttcg aaaaaagacg ctgaaaagcg tcttttttcg
      421 ttttggtccg gagtaatacg actcactata gtactagacc acaacggttt ccctctagaa
      481 ataattttgt ttaactttaa gaaggagata tacataatgg ttttcacttt agaagacttc
      541 gtaggtgact ggcgtcaaac agcaggttat aatttagacc aagttttaga acaaggtggt
      601 gtatcaagtt tattccaaaa tttaggtgtt tctgttactc caattcaacg tatcgtatta
      661 agtggtgaaa atggtcttaa aattgacatc catgttatta ttccttatga aggtctttca
      721 ggtgaccaaa tgggtcaaat tgaaaaaatt tttaaagtag tttatcctgt agatgaccat
      781 cactttaaag taattttaca ctatggtact ttagtaattg atggcgttac acctaatatg
      841 attgactact ttggtcgtcc ttatgaaggt attgctgttt ttgatggtaa aaaaatcaca
      901 gttacaggta cattatggaa tggtaataaa attattgacg aacgtttaat caatcctgat
      961 ggttcattat tattccgtgt tacaattaat ggtgttacag gttggcgtct ttgtgaacgt
     1021 atccttgctg cttaaataat aaataacgga ttgtgtccgt aatcacacgt ggtgcgtacg
     1081 ataacgcata gtgtttttcc ctccacttaa atcgaagggt tgtgtcttgg atcgcgcggg
     1141 tcaaatgtat atggttcata tacatccgca ggcacgtaat aaagcgaggg gttcgaatcc
     1201 ccccgttacc cccggtaggg gcccacgctt actggagacg agctgcggcc gcgattatca
     1261 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1321 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1381 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1441 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggga cccacgctca
     1501 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1561 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1621 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1681 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1741 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1801 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1861 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1921 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1981 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2041 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2101 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2161 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2221 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2281 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgtc
     2341 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2401 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2461 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2521 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2581 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2641 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2701 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2761 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2821 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2881 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2941 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3001 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3061 atgttctttc ctgcgttatc ccctgattct gtggataacc gtgcggccgc
//
