LOCUS       pJWK19-119_(hSYN1::Cal- 5906 bp ds-DNA     circular     02-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..130
                     /label="L-ITR"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    40..45
                     /label="SmaI"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    139..144
                     /label="MluI"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    145..150
                     /label="BsrGI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     exon            151..635
                     /label="Human Synapsin I promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    151..641
                     /label="fp"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    151..641
                     /label="oligo fp"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          543..560
                     /label="AAV Cal-ID Seq 1"
                     /note="sequence: accatctgcgctgcggcg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    636..641
                     /label="BamHI"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    672..707
                     /label="AviTag* (T-2)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             672..707
                     /label="Translation 672-707"
                     /translation="NDIFEAQRIEWH"
     misc_feature    708..770
                     /label="M13 (chicken smooth muscle)"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             708..770
                     /label="Translation 708-770"
                     /translation="SSRRKWNKTGHAVRAIGRLSS"
     misc_feature    771..794
                     /label="Xpress"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             771..794
                     /label="Translation 771-794"
                     /translation="DLYDDDDK"
     CDS             795..800
                     /label="Translation 795-800"
                     /translation="LE"
     misc_feature    801..1532
                     /label="cpTurbo2_C"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             897..899
                     /label="Translation 897-899"
                     /translation="Y"
     CDS             900..920
                     /label="Translation 900-920"
                     /translation="QQAGRGS"
     CDS             918..920
                     /label="Translation 918-920"
                     /translation="S"
     CDS             921..941
                     /label="Translation 921-941"
                     /translation="RGRKWFS"
     misc_feature    1146..1148
                     /label="cutPoint(N)"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1149..1151
                     /label="cutPoint(C)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1235..1254
                     /label="AAV Cal-ID Seq 2"
                     /note="sequence: cacactgcaggaagcaggga"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1533..1550
                     /label="Linker"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             1533..1550
                     /label="Translation 1533-1550"
                     /translation="GGTGGS"
     misc_feature    1551..1781
                     /label="cpTurbo2_N"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1782..1787
                     /label="LP linker"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1788..2228
                     /label="CaM (3-148)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          2070..2089
                     /label="AAV Cal-ID Seq 3"
                     /note="sequence: GGCAATGGCTACATCAGTGC"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             2229..2270
                     /label="V5 tag"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2274..2279
                     /label="EcoRI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2288..2896
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2904..2909
                     /label="XhoI"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    2910..3141
                     /label="bGH polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3142..3148
                     /label="BstEII"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3154..3160
                     /label="RsrII"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3170..3310
                     /label="R-ITR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      3401..3707
                     /label="f1 Origin"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4226..5083
                     /label="AmpR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      5234..5901
                     /label="pUC ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtTGTACA gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cGGGCTGCAG GTCGACTCTA
      661 GAGCCACCAT GAACGACATC TTCGAGGCTC AGCGTATCGA ATGGCACTCA TCACGTCGTA
      721 AGTGGAATAA GACAGGTCAC GCAGTCAGAG CTATAGGTCG GCTGAGCTCA GATCTGTACG
      781 ACGATGACGA TAAGCTCGAG gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc
      841 agtacctgct ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc
      901 agcaggcagg gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt
      961 acctgagtat gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca
     1021 tcggaattgt catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat
     1081 ggcccaatga cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg
     1141 gaataacagg cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc
     1201 gcgtggagga aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc
     1261 tggacaggaa tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt
     1321 tcgagcagga aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca
     1381 atagacccgt gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg
     1441 ataaacaggg agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag
     1501 aaatcagtct gcggtctgcc gaaaagctgc agGGCGGTAC CGGAGGGAGC aaagacaata
     1561 ctgtgcctct gaagctgatc gctctcctgg ctaatggcga gttccatagt ggcgaacagc
     1621 tgggagaaac cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg
     1681 actggggcgt ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc
     1741 cgctgctgaa cgctaaacag attctgggac agctggacgg cCTGCCGGAC CAACTGACTG
     1801 AAGAGCAGAT CGCAGAATTT AAAGAGGCTT TCTCCCTATT TGACAAGGAC GGGGATGGGA
     1861 CAATAACAAC CAAGGAGCTG GGGACGGTGA TGCGGTCTCT GGGGCAGAAC CCCACAGAAG
     1921 CAGAGCTGCA GGACATGATC AATGAAGTAG ATGCCGACGG TGACGGCACA ATCGACTTCC
     1981 CTGAGTTCCT GACAATGATG GCAAGAAAAA TGAAATACAG GGACACGGAA GAAGAAATTA
     2041 GAGAAGCGTT CGGTGTGTTT GATAAGGATG GCAATGGCTA CATCAGTGCA GCAGAGCTTC
     2101 GCCACGTGAT GACAAACCTT GGAGAGAAGT TAACAGATGA AGAGGTTGAT GAAATGATCA
     2161 GGGAAGCAGA CATCGATGGG GATGGTCAGG TAAACTACGA AGAGTTTGTA CAAATGATGA
     2221 CAGCGAAGgg caagcccatc cccaaccccc tgctgggcct ggacagcacc TGAgaattcg
     2281 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2341 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2401 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2461 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2521 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2581 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2641 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2701 cctttccttg gctgctcgcc tAtgttgcca cctggattct gcgcgggacg tccttctgct
     2761 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2821 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2881 ccccgcatcg ataccgagcg ctgctcgagC TAGAGCTCGC TGATCAGCCT CGACTGTGCC
     2941 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG
     3001 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG
     3061 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA
     3121 CAATAGCAGG CATGCTGGGG Aggtaaccac gtgcggaccg agcggccgca ggaaccccta
     3181 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     3241 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     3301 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tattcacacc
     3361 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3421 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3481 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3541 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
     3601 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3661 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3721 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3781 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
     3841 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     3901 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     3961 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4021 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4081 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4141 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4201 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4261 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4321 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4381 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4441 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4501 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4561 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4621 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4681 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4741 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4801 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4861 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4921 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4981 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5041 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5101 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5161 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5221 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5281 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5341 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5401 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5461 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5521 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5581 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5641 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5701 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5761 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5821 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5881 cttttgctgg ccttttgctc acatgt
//