LOCUS       pYB-Dual(mNG(yeast-opt))      7643 bp    DNA     circular UNA 
FEATURES             Location/Qualifiers
     CDS             424..1518
                     /label="LEU2"
     misc_feature    complement(2367..2673)
                     /feature_type="Misc."
                     /region_type="Alpha-helix"
                     /label="F1 origin"
     terminator      complement(2908..3102)
                     /label="tADH1"
     promoter        3121..3656
                     /feature_type="Bulge"
                     /label="pGAL1"
     promoter        3666..3684
                     /label="T7 RNAP promotor"
     misc_feature    3691..3704
                     /feature_type="Misc."
                     /label="SD (bacteria)"
     misc_feature    3703..3710
                     /feature_type="Misc."
                     /label="Yeast translation increase"
     CDS             3711..4421
                     /label="mNeonGreen(yeast-optimized)"
     terminator      4441..4611
                     /label="tCYC1"
     CDS             complement(5830..6690)
                     /label="AmpR"
ORIGIN
        1 CACCATTATG GGAAATGGTT CAAGAAGGTA TTGACTTAAA CTCCATCAAA TGGTCAGGTC
       61 ATTGAGTGTT TTTTATTTGT TGTATTTTTT TTTTTTTAGA GAAAATCCTC CAATATCAAA
      121 TTAGGAATCG TAGTTTCATG ATTTTCTGTT ACACCTAACT TTTTGTGTGG TGCCCTCCTC
      181 CTTGTCAATA TTAATGTTAA AGTGCAATTC TTTTTCCTTA TCACGTTGAG CCATTAGTAT
      241 CAATTTGCTT ACCTGTATTC CTTTACTATC CTCCTTTTTC TCCTTCTTGA TAAATGTATG
      301 TAGATTGCGT ATATAGTTTC GTCTACCCTA TGAACATATT CCATTTTGTA ATTTCGTGTC
      361 GTTTCTATTA TGAATTTCAT TTATAAAGTT TATGTACAAA TATCATAAAA AAAGAGAATC
      421 TTTTTAAGCA AGGATTTTCT TAACTTCTTC GGCGACAGCA TCACCGACTT CGGTGGTACT
      481 GTTGGAACCA CCTAAATCAC CAGTTCTGAT ACCTGCATCC AAAACCTTTT TAACTGCATC
      541 TTCAATGGCC TTACCTTCTT CAGGCAAGTT CAATGACAAT TTCAACATCA TTGCAGCAGA
      601 CAAGATAGTG GCGATAGGGT CAACCTTATT CTTTGGCAAA TCTGGAGCAG AACCGTGGCA
      661 TGGTTCGTAC AAACCAAATG CGGTGTTCTT GTCTGGCAAA GAGGCCAAGG ACGCAGATGG
      721 CAACAAACCC AAGGAACCTG GGATAACGGA GGCTTCATCG GAGATGATAT CACCAAACAT
      781 GTTGCTGGTG ATTATAATAC CATTTAGGTG GGTTGGGTTC TTAACTAGGA TCATGGCGGC
      841 AGAATCAATC AATTGATGTT GAACCTTCAA TGTAGGGAAT TCGTTCTTGA TGGTTTCCTC
      901 CACAGTTTTT CTCCATAATC TTGAAGAGGC CAAAACATTA GCTTTATCCA AGGACCAAAT
      961 AGGCAATGGT GGCTCATGTT GTAGGGCCAT GAAAGCGGCC ATTCTTGTGA TTCTTTGCAC
     1021 TTCTGGAACG GTGTATTGTT CACTATCCCA AGCGACACCA TCACCATCGT CTTCCTTTCT
     1081 CTTACCAAAG TAAATACCTC CCACTAATTC TCTGACAACA ACGAAGTCAG TACCTTTAGC
     1141 AAATTGTGGC TTGATTGGAG ATAAGTCTAA AAGAGAGTCG GATGCAAAGT TACATGGTCT
     1201 TAAGTTGGCG TACAATTGAA GTTCTTTACG GATTTTTAGT AAACCTTGTT CAGGTCTAAC
     1261 ACTACCGGTA CCCCATTTAG GACCACCCAC AGCACCTAAC AAAACGGCAT CAACCTTCTT
     1321 GGAGGCTTCC AGCGCCTCAT CTGGAAGTGG GACACCTGTA GCATCGATAG CAGCACCACC
     1381 AATTAAATGA TTTTCGAAAT CGAACTTGAC ATTGGAACGA ACATCAGAAA TAGCTTTAAG
     1441 AACCTTAATG GCTTCGGCTG TGATTTCTTG ACCAACGTGG TCACCTGGCA AAACGACGAT
     1501 CTTCTTAGGG GCAGACATAG GGGCAGACAT TAGAATGGTA TATCCTTGAA ATATATATAT
     1561 ATATTGCTGA AATGTAAAAG GTAAGAAAAG TTAGAAAGTA AGACGATTGC TAACCACCTA
     1621 TTGGAAAAAA CAATAGGTCC TTAAATAATA TTGTCAACTT CAAGTATTGT GATGCAAGCA
     1681 TTTAGTCATG AACGCTTCTC TATTCTATAT GAAAAGCCGG TTCCGGCCTC TCACCTTTCC
     1741 TTTTTCTCCC AATTTTTCAG TTGAAAAAGG TATATGCGTC AGGCGACCTC TGAAATTAAC
     1801 AAAAAATTTC CAGTCATCGA ATTTGATTCT GTGCGATAGC GCCCCTGTGT GTTCTCGTTA
     1861 TGTTGAGGAA AAAAATAATG GTTGCTAAGA GATTCGAACT CTTGCATCTT ACGATACCTG
     1921 AGTATTCCCA CAGTTAACTG CGGTCAAGAT ATTTCTTGAA TCAGGCGCCT TAGACCGCTC
     1981 GGCCAAACAA CCAATTACTT GTTGAGAAAT AGAGTATAAT TATCCTATAA ATATAACGTT
     2041 TTTGAACACA CATGAACAAG GAAGTACAGG ACAATTGATT TTGAAGAGAA TGTGGATTTT
     2101 GATGTAATTG TTGGGATTCC ATTTTTAATA AGGCAATAAT ATTAGGTATG TGGATATACT
     2161 AGAAGTTCTC CTCGACCGTC GATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA
     2221 AATACCGCAT CAGGAAATTG TAAACGTTAA TATTTTGTTA AAATTCGCGT TAAATTTTTG
     2281 TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC AAAATCCCTT ATAAATCAAA
     2341 AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC CACTATTAAA
     2401 GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT CAGGGCGATG GCCCACTACG
     2461 TGAACCATCA CCCTAATCAA GTTTTTTGGG GTCGAGGTGC CGTAAAGCAC TAAATCGGAA
     2521 CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA
     2581 GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT
     2641 GCGCGTAACC ACCACACCCG CCGCGCTTAA TGCGCCGCTA CAGGGCGCGT CGCGCCATTC
     2701 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG
     2761 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC
     2821 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGAGCGCGCG TAATACGACT CACTATAGGG
     2881 CGAATTGGGT ACCGGGCCCC CCCTCGAGAG CGACCTCATG CTATACCTGA GAAAGCAACC
     2941 TGACCTACAG GAAAGAGTTA CTCAAGAATA AGAATTTTCG TTTTAAAACC TAAGAGTCAC
     3001 TTTAAAATTT GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC
     3061 ATAAGAAATT CGCTTATTTA GAAGTGTCAA CAACGTATCT ACCAACGGAA TGCGTGCGAT
     3121 GAGCCCCATT ATCTTAGCCT AAAAAAACCT TCTCTTTGGA ACTTTCAGTA ATACGCTTAA
     3181 CTGCTCATTG CTATATTGAA GTACGGATTA GAAGCCGCCG AGCGGGCGAC AGCCCTCCGA
     3241 CGGAAGACTC TCCTCCGTGC GTCCTGGTCT TCACCGGTCG CGTTCCTGAA ACGCAGATGT
     3301 GCCTCGCGCC GCACTGCTCC GAACAATAAA GATTCTACAA TACTAGCTTT TATGGTTATG
     3361 AAGAGGAAAA ATTGGCAGTA ACCTGGCCCC ACAAACCTTC AAATCAACGA ATCAAATTAA
     3421 CAACCATAGG ATAATAATGC GATTAGTTTT TTAGCCTTAT TTCTGGGGTA ATTAATCAGC
     3481 GAAGCGATGA TTTTTGATCT ATTAACAGAT ATATAAATGC AAAAGCTGCA TAACCACTTT
     3541 AACTAATACT TTCAACATTT TCGGTTTGTA TTACTTCTTA TTCAAATGTC ATAAAAGTAT
     3601 CAACAAAAAA TTGTTAATAT ACCTCTATAC TTTAACGTCA AGGAGAAAAA ACTATAATCT
     3661 GTCATTAATA CGACTCACTA TAGGTCTAGA GAAAGAGGGG AGAATTAAAA ATGGTGTCTA
     3721 AAGGTGAAGA GGACAACATG GCTTCTTTGC CAGCTACTCA TGAATTGCAT ATTTTCGGTT
     3781 CCATCAACGG TGTTGATTTC GATATGGTTG GTCAAGGTAC TGGTAATCCA AATGATGGTT
     3841 ACGAAGAGTT GAACCTGAAA TCTACAAAAG GTGACTTGCA ATTCTCCCCA TGGATTTTGG
     3901 TTCCACATAT TGGTTACGGT TTCCACCAAT ATTTGCCATA TCCAGATGGT ATGTCTCCAT
     3961 TTCAAGCTGC TATGGTTGAT GGTTCTGGTT ACCAAGTTCA TAGAACCATG CAATTTGAAG
     4021 ATGGTGCTTC TTTGACCGTC AACTACAGAT ATACTTACGA AGGTTCCCAT ATTAAGGGTG
     4081 AAGCTCAAGT AAAAGGTACA GGTTTTCCAG CTGATGGTCC AGTTATGACT AATTCTTTGA
     4141 CTGCTGCTGA TTGGTGCAGA TCCAAAAAGA CTTACCCAAA CGATAAGACC ATCATCTCTA
     4201 CTTTCAAGTG GTCTTACACT ACTGGTAACG GTAAGAGATA TAGATCTACT GCTAGAACTA
     4261 CTTACACCTT CGCTAAACCT ATGGCTGCTA ACTACTTGAA GAATCAACCT ATGTACGTGT
     4321 TCAGAAAGAC CGAATTGAAA CACTCCAAAA CCGAGTTGAA CTTCAAAGAA TGGCAAAAGG
     4381 CTTTCACCGA TGTTATGGGT ATGGATGAAT TATACAAATA AGGATCCATC GCGTGCATTC
     4441 ATCCGCTCTA ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT
     4501 TTTTATAGTT ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT
     4561 GTACAGACGC GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGAGCTCCAG
     4621 CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT
     4681 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATAGGAGCCG GAAGCATAAA
     4741 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG GTAACTCACA TTAATTGCGT TGCGCTCACT
     4801 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC
     4861 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG
     4921 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC
     4981 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
     5041 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA
     5101 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA
     5161 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG
     5221 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG
     5281 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
     5341 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA
     5401 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG
     5461 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT
     5521 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC
     5581 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
     5641 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG
     5701 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA
     5761 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG
     5821 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG
     5881 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
     5941 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC
     6001 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC
     6061 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG
     6121 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT
     6181 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG
     6241 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT
     6301 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG
     6361 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG
     6421 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT
     6481 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT
     6541 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC
     6601 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT
     6661 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT
     6721 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA
     6781 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GGGTCCTTTT CATCACGTGC
     6841 TATAAAAATA ATTATAATTT AAATTTTTTA ATATAAATAT ATAAATTAAA AATAGAAAGT
     6901 AAAAAAAGAA ATTAAAGAAA AAATAGTTTT TGTTTTCCGA AGATGTAAAA GACTCTAGGG
     6961 GGATCGCCAA CAAATACTAC CTTTTATCTT GCTCTTCCTG CTCTCAGGTA TTAATGCCGA
     7021 ATTGTTTCAT CTTGTCTGTG TAGAAGACCA CACACGAAAA TCCTGTGATT TTACATTTTA
     7081 CTTATCGTTA ATCGAATGTA TATCTATTTA ATCTGCTTTT CTTGTCTAAT AAATATATAT
     7141 GTAAAGTACG CTTTTTGTTG AAATTTTTTA AACCTTTGTT TATTTTTTTT TCTTCATTCC
     7201 GTAACTCTTC TACCTTCTTT ATTTACTTTC TAAAATCCAA ATACAAAACA TAAAAATAAA
     7261 TAAACACAGA GTAAATTCCC AAATTATTCC ATCATTAAAA GATACGAGGC GCGTGTAAGT
     7321 TACAGGCAAG CGATCCGTCC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA
     7381 GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA AACCTCTGAC
     7441 ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG AGCAGACAAG
     7501 CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC TATGCGGCAT
     7561 CAGAGCAGAT TGTACTGAGA GTGCACCATA TCGACTACGT CGTAAGGCCG TTTCTGACAG
     7621 AGTAAAATTC TTGAGGGAAC TTT
//
