LOCUS       mCherry-LgBiT-tP       13158 bp DNA     circular SYN 15-FEB-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Feb 15, 2024 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13158
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      1..589
                     /label=ori
                     /note="direction: RIGHT note: high-copy-number 
                     ColE1/pMB1/pBR322/pUC origin of replication"
     protein_bind    877..898
                     /label=CAP binding site
                     /note="bound_moiety: E. coli catabolite activator protein 
                     note: CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        913..918
                     /label=lac promoter
                     /note="note: promoter for the E. coli lac operon"
     promoter        919..936
                     /label=lac promoter
                     /note="note: promoter for the E. coli lac operon"
     promoter        937..943
                     /label=lac promoter
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /note="bound_moiety: lac repressor encoded by lacI note: 
                     The lac repressor binds to the lac operator to inhibit 
                     transcription in E. coli. This inhibition can be relieved 
                     by adding lactose or isopropyl-beta-D-thiogalactopyranoside
                     (IPTG)."
     primer_bind     975..991
                     /label=M13 rev
                     /note="note: common sequencing primer, one of multiple 
                     similar variants"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="note: promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1074..1386
                     /label=ePiggyBac left (5') inverted repeat
     misc_feature    1250
                     /label=ePB-C136T
     misc_feature    1333
                     /label=ePB-T53C
     polyA_signal    1400..1521
                     /label=SV40 poly(A) signal
                     /note="note: SV40 polyadenylation signal"
     CDS             complement(1568..2167)
                     /label=PuroR
                     /note="codon_start: 1 gene: pac from Streptomyces alboniger
                     note: confers resistance to puromycin product: puromycin 
                     N-acetyltransferase transl_table: 1 translation: 
                     MAEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGL
                     DIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRP
                     KEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFT
                     VTADVEVPEGPRTWCMTRKPGA*"
     promoter        complement(2186..2233)
                     /label=EM7 promoter
                     /note="note: synthetic bacterial promoter "
     promoter        complement(2242..3451)
                     /label=UbC promoter
                     /note="note: human ubiquitin C promoter"
     misc_feature    3470..5188
                     /label=CAG promoter
     enhancer        3470..3852
                     /label=CMV enhancer
                     /note="note: human cytomegalovirus immediate early 
                     enhancer"
     promoter        3854..4129
                     /label=chicken beta-actin promoter
     intron          4130..5140
                     /label=chimeric intron
     misc_feature    4871..5216
                     /label=seq conf 230407
     misc_feature    5210..5215
                     /label=HindIII
     CDS             5223..5930
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             6426..6482
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6492..6545
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6546..7259
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             7314..10820
                     /codon_start=1
                     /label=Vinculin gallus
                     /translation="MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVS
                     AVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVRAAQMLQADPYSVPA
                     RDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKN
                     LGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNTKSQG
                     IEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALALIDSKMNQAK
                     GWLRDPNAPPGDAGEQAIRQILDEAGKAGELCAGKERREILGTCKTLGQMTDQLADLRA
                     RGQGATPMAMQKAQQVSQGLDLLTAKVENAARKLEAMTNSKQAIAKKIDAAQNWLADPN
                     GGSEGEEHIRGIMSEARKVAELCEEPKERDDILRSLGEISALTAKLSDLRRHGKGDSPE
                     ARALAKQIATSLQNLQSKTNRAVANTRPVKAAVHLEGKIEQAQRWIDNPTVDDRGVGQA
                     AIRGLVAEGRRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGESPQARAIAAQL
                     QDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPSDTPNREEVFEERAANFEN
                     HAARLGATAEKAAAVGTANKTTVEGIQATVKSARELTPQVVSAARILLRNPGNQAAYEH
                     FETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANMQPQMLVAGA
                     TSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPG
                     LQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEHLHLTDELAPPKPPLPEGEVP
                     PPRPPPPEEKDEEFPEQKAGEAINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLM
                     AEMSRLVRGGSGNKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIP
                     TISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKI
                     RTDAGFTLRWVRKTPWYQEFMETLHITKTMKNIEVPETKTASFECEVSHFNVPSMWLKN
                     GVEIEMSEKFKIVVQGKLHQLIIMNTSTEDSAEYTFVCGVSGWRLFKKISNDQVSATLT
                     VT"
     CDS             10518..10816
                     /codon_start=1
                     /label=STReTCh
                     /translation="METLHITKTMKNIEVPETKTASFECEVSHFNVPSMWLKNGVEIEM
                     SEKFKIVVQGKLHQLIIMNTSTEDSAEYTFVCGVSGWRLFKKISNDQVSATLTV"
     CDS             10752..10784
                     /codon_start=1
                     /label=SmBiT_HiAff
                     /translation="VSGWRLFKKIS"
     misc_feature    10830..10837
                     /label=NotI
     polyA_signal    10951..11072
                     /label=SV40 poly(A) signal
                     /note="note: SV40 polyadenylation signal"
     misc_feature    11083..11324
                     /label=ePiggyBac right (3') inverted repeat
     promoter        complement(11356..11374)
                     /label=T7 promoter
                     /note="note: promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11384..11400)
                     /label=M13 fwd
                     /note="note: common sequencing primer, one of multiple 
                     similar variants"
     rep_origin      11542..11997
                     /label=f1 ori
                     /note="direction: RIGHT note: f1 bacteriophage origin of 
                     replication; arrow indicates direction of (+) strand 
                     synthesis"
     promoter        12023..12127
                     /label=AmpR promoter
                     /note="gene: bla"
     CDS             12128..12196
                     /label=AmpR
                     /note="codon_start: 1 gene: bla note: confers resistance to
                     ampicillin, carbenicillin, and related antibiotics product:
                     beta-lactamase transl_table: 1 translation: 
                     MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESF
                     RPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTV
                     RELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDER
                     DTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGA
                     GERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             12197..12988
                     /label=AmpR
                     /note="codon_start: 1 gene: bla note: confers resistance to
                     ampicillin, carbenicillin, and related antibiotics product:
                     beta-lactamase transl_table: 1 translation: 
                     MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESF
                     RPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTV
                     RELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDER
                     DTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGA
                     GERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctcca ccgcggggcg cgccgcagct agattaaccc
     1081 tagaaagata gtctgcgtaa aattgacgca tgcattcttg aaatattgct ctctctttct
     1141 aaatagcgcg aatccgtcgc tgtgcattta ggacatctca gtcgccgctt ggagctcccg
     1201 tgaggcgtgc ttgtcaatgc ggtaagtgtc actgattttg aactataaca accgcgtgag
     1261 tcaaaatgac gcatgattat cttttacgtg acttttaaga tttaactcat acgataatta
     1321 tattgttatt tcgtgttcta cttacgtgat aacttattat atatatattt tcttgttata
     1381 gatgggaatt cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1441 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1501 ataagctgca ataaacaagt ttcgaggtcg agtgtcagtc ctgctcctcg gccacgaagt
     1561 gcttagctca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     1621 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     1681 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     1741 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     1801 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     1861 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     1921 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     1981 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     2041 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     2101 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     2161 cggccatggt ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg
     2221 attaattgtc aacacggtcc ggtctaacaa aaaagccaaa aacggccaga atttagcgga
     2281 caatttacta gtctaacact gaaaattaca tattgaccca aatgattaca tttcaaaagg
     2341 tgcctaaaaa acttcacaaa acacactcgc caaccccgag cgcatagttc aaaaccggag
     2401 cttcagctac ttaagaagat aggtacataa aaccgaccaa agaaactgac gcctcactta
     2461 tccctcccct caccagaggt ccggcgcctg tcgattcagg agagcctacc ctaggcccga
     2521 accctgcgtc ctgcgacgga gaaaagccta ccgcacacct accggcaggt ggccccaccc
     2581 tgcattataa gccaacagaa cgggtgacgt cacgacacga cgagggcgcg cgctcccaaa
     2641 ggtacgggtg cactgcccaa cggcaccgcc ataactgccg cccccgcaac agacgacaaa
     2701 ccgagttctc cagtcagtga caaacttcac gtcagggtcc ccagatggtg ccccagccca
     2761 tctcacccga ataagagctt tcccgcatta gcgaaggcct caagaccttg ggttcttgcc
     2821 gcccaccatg ccccccacct tgtttcaacg acctcacagc ccgcctcaca agcgtcttcc
     2881 attcaagact cgggaacagc cgccattttg ctgcgctccc cccaaccccc agttcagggc
     2941 aaccttgctc gcggacccag actacagccc ttggcggtct ctccacacgc ttccgtccca
     3001 ccgagcggcc cggcggccac gaaagccccg gccagcccag cagcccgcta ctcaccaagt
     3061 gacgatcaca gcgatccaca aacaagaacc gcgacccaaa tcccggctgc gacggaacta
     3121 gctgtgccac acccggcgcg tccttatata atcatcggcg ttcaccgccc cacggagatc
     3181 cctccgcaga atcgccgaga agggactact tttcctcgcc tgttccgctc tctggaaaga
     3241 aaaccagtgc cctagagtca cccaagtccc gtcctaaaat gtccttctgc tgatactggg
     3301 gttctaaggc cgagtcttat gagcagcggg ccgctgtcct gagcgtccgg gcggaaggat
     3361 caggacgctc gtgcgccctt cgtctgacgt ggcagcgctc gccgtgagga gggggcgccc
     3421 gcggggggcg ccaaaacccg gcgcggaggc cagatctccc gatctcgagg tcgacattga
     3481 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     3541 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     3601 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3661 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3721 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3781 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3841 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     3901 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     3961 gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag
     4021 gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc
     4081 gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc
     4141 gttgccttcg ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact
     4201 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     4261 gcgcttggtt taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct
     4321 ccgggagggc cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     4381 tggggagcgc cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     4441 ggggctttgt gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg
     4501 tgcggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag
     4561 cagggggtgt gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt
     4621 gctgagcacg gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc
     4681 gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc
     4741 ggggagggct cgggggaggg gcgcggcggc cccggagcgc cggcggctgt cgaggcgcgg
     4801 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt
     4861 cccaaatctg gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg
     4921 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     4981 gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct
     5041 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     5101 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     5161 tattgtgctg tctcatcatt ttggcaaaga attcctcgac ggtatcgata agcttggcca
     5221 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     5281 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     5341 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     5401 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     5461 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     5521 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     5581 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     5641 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     5701 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     5761 ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     5821 tcaacatcaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     5881 agcgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggaagcggag
     5941 tcttcactct ggaagatttc gtaggtgact gggagcaaac agcagcatac aatcttgatc
     6001 aagtgttgga gcaagggggg gtaagctctc tcctccaaaa cttggctgtc tcagttactc
     6061 caattcaacg aattgtacgg tcaggggaaa atgcactcaa gattgacatt catgtcatca
     6121 ttccttatga gggtctgagt gccgatcaga tggcccagat cgaggaggtt tttaaggtag
     6181 tgtatcccgt ggatgatcat cactttaagg taattttgcc ctacggaact ctggtaattg
     6241 acggagtgac ccccaatatg ctcaattatt ttggtaggcc ctatgaaggt attgctgtct
     6301 tcgacggaaa aaaaataaca gttactggga cactctggaa tggtaataag attatagacg
     6361 aacgcttgat cactcctgac ggcagtatgt tgttccgcgt taccataaat gggtctggaa
     6421 gcggagccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc
     6481 ctggaagcgg agagggcagg ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg
     6541 gccccgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6601 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     6661 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6721 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     6781 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     6841 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     6901 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     6961 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     7021 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     7081 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     7141 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     7201 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     7261 ccggactcgg aggaagcgga ggtggaggaa gcggaggtgg aggaagcgga ggtatgcccg
     7321 tcttccacac gcgcaccatc gagagcatct tggagcccgt ggctcagcag atctcccacc
     7381 tggtcatcat gcacgaggag ggggaggtag acggcaaggc catcccggac ctcaccgccc
     7441 ccgtgtcggc cgtgcaggcc gctgtcagca acctggtgcg ggttggaaaa gaaactgtgc
     7501 agacaacaga agaccagatc ttgaaaaggg atatgccacc agcattcatc aaagtagaga
     7561 atgcctgcac caagctcgtt cgagcagccc agatgctgca agcagatcct tattcagtac
     7621 cagctcgtga ctacctaatt gatggatcaa gaggcatcct ttctggaaca tcagacttac
     7681 ttctgacatt tgatgaagcg gaggtccgta aaatcatccg tgtctgcaaa ggaatattgg
     7741 aatatctgac tgtggcagaa gtagtagaga ctatggagga tttggtgaca tatacaaaga
     7801 atctagggcc aggaatgaca aagatggcga aaatgatcga tgagagacaa caggaattaa
     7861 ctcatcagga acatagggtt atgctggtga actccatgaa tactgtgaag gagctattgc
     7921 ctgtacttat ttcagctatg aagatctttg taaccacaaa aaacactaaa agccagggaa
     7981 tagaagaggc cttgaaaaat cgcaatttca cagtagagaa aatgagtgct gagataaatg
     8041 aaataatccg tgtattacaa ctcacttcct gggatgaaga tgcctgggcc agcaaggaca
     8101 ctgaagccat gaaaagagct ttagccctaa tagattcaaa gatgaaccag gcaaaaggct
     8161 ggctgagaga tccaaacgca cctccagggg atgctggtga gcaagcaata aggcagatcc
     8221 ttgatgaagc tggaaaagca ggagaattgt gtgcaggcaa agaacgcagg gagattctgg
     8281 gaacatgcaa aactttgggc caaatgactg atcaacttgc tgatctccga gctagaggac
     8341 agggtgctac accgatggca atgcagaagg cacagcaggt gtcacaaggc ctggatttgc
     8401 tcactgcaaa agtggagaat gcagcccgga aattggaggc catgacaaac tctaagcagg
     8461 ctattgcaaa gaagattgat gctgctcaga actggcttgc ggatcccaac ggcggaagtg
     8521 aaggagaaga acacattcga ggaattatgt ctgaagcaag gaaagttgca gaattgtgtg
     8581 aggagcctaa agaaagagat gatatccttc gctccttggg ggaaatctct gctctgacag
     8641 ctaagctgtc agatctgcga cgacatggga aaggcgactc tcctgaggcc cgtgcattgg
     8701 ccaagcaaat agctacatca cttcagaact tacagtccaa aacaaacaga gctgtagcaa
     8761 atactaggcc agttaaagct gctgtccatt tggagggcaa gattgagcaa gcccagaggt
     8821 ggatagataa tcctacagtt gatgatcggg gagtaggcca ggcagcaatt cggggtttgg
     8881 ttgcagaagg tcgtcgtcta gccaatgtca tgatgggacc ttatcgtcag gacctgcttg
     8941 ccaaatgtga ccgtgtagac cagctggctg ctcagcttgc tgaccttgca gcaagagggg
     9001 aaggagagtc tcctcaggct agggcaattg ctgctcagct tcaggactcc ctgaaggatc
     9061 tcaaagcacg gatgcaagaa gcaatgaccc aggaggtgtc tgatgttttc agtgacacta
     9121 caacccctat taagttgtta gcagtagcag ccactgctcc ttctgatact cccaatagag
     9181 aagaggtgtt tgaagaaaga gcagcaaatt ttgaaaacca tgctgctaga ctgggagcaa
     9241 cagcagaaaa agcagctgca gttggaactg ctaataaaac tactgtggaa ggcattcagg
     9301 caacagtcaa atctgcaagg gagcttacac cacaggtagt atcggctgct cgaatcctcc
     9361 tgagaaatcc tggaaatcaa gctgcttatg agcattttga gacaatgaaa aaccaatgga
     9421 ttgataatgt agaaaagatg acagggctgg tggatgaggc catcgatacc aagtctctgt
     9481 tggatgcatc agaagaggct attaagaagg atcttgataa atgcaaagtt gcaatggcca
     9541 atatgcaacc tcagatgctg gtagctggag ccaccagcat tgctagacga gcgaaccgca
     9601 tcctgcttgt ggcaaaacgg gaggttgaaa attcagaaga ccctaaattc agggaggctg
     9661 ttaaagcagc ttctgatgag ctgagcaaaa ccatatcacc gatggtaatg gatgctaaag
     9721 ctgtagcagg aaatatctct gatcctggtt tgcagaagag tttcttggat tctggataca
     9781 ggattctggg agctgtggcc aaagtcagag aagcctttca gcctcaggag ccagactttc
     9841 cccctcctcc tcctgaccta gagcatctcc atctgactga tgagcttgct cctccaaaac
     9901 caccacttcc agaaggtgag gttcccccac ccagaccacc accccctgaa gaaaaagatg
     9961 aggagttccc agagcagaaa gcaggagaag ctattaatca gcccatgatg atggctgcta
    10021 ggcagttgca tgacgaggct cggaaatggt ctagcaaggg taacgacatc attgctgctg
    10081 ctaaacgaat ggcgctgctc atggctgaga tgtcacgcct ggtgcgagga ggcagcggaa
    10141 acaagcgtgc cctcatccag tgtgcaaaag atattgctaa ggcatcggat gaagtcactc
    10201 gattggccaa agaggtggcc aagcaatgta ctgataaacg cattagaaca aacctcttac
    10261 aggtctgtga gcgaatccca accatcagca cgcagctcaa aattctttcc acagtgaaag
    10321 ctaccatgct gggcaggact aacatcagcg atgaagaatc agaacaggca actgagatgt
    10381 tggttcataa cgcccagaac ctcatgcagt ctgtgaagga aactgtgaga gaagctgaag
    10441 cagcatccat taagataaga acagatgccg gattcactct gcgctgggtc agaaagaccc
    10501 catggtatca ggaattcatg gaaactctgc atattaccaa aaccatgaaa aacattgaag
    10561 tgccggaaac caaaaccgcg agctttgaat gcgaagtgag ccattttaac gtgccgagca
    10621 tgtggctgaa aaacggcgtg gaaattgaaa tgagcgaaaa atttaaaatt gtggtgcagg
    10681 gcaaactgca tcagctgatt attatgaaca ccagcaccga agatagcgcg gaatatacct
    10741 ttgtgtgcgg cgttagtggc tggcgtctgt ttaagaaaat tagcaacgat caggtgagcg
    10801 cgaccctgac cgtgacttag tttaattaag cggccgcgac tctagatcat aatcagccat
    10861 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
    10921 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
    10981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    11041 tgtggtttgt ccaaactcat caatgtatct taaggaattc gataaaagtt ttgttacttt
    11101 atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt
    11161 ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa
    11221 ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat
    11281 tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagc tgcggcgcgc
    11341 cggtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
    11401 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    11461 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    11521 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    11581 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    11641 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    11701 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    11761 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    11821 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    11881 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
    11941 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
    12001 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    12061 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
    12121 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
    12181 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    12241 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    12301 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    12361 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    12421 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    12481 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    12541 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    12601 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    12661 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    12721 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    12781 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    12841 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    12901 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    12961 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    13021 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    13081 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    13141 aaagatcaaa ggatcttc
//
