LOCUS       1296.pENTR-myc-t        8732 bp    DNA     linear   UNA 30-JUL-2015
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 13, 2025 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    569..667
                     /label=attL1
     misc_feature    join(652..686,6856..6866)
                     /label=transferred sequence
     misc_feature    652..686
                     /label=Trabsferred sequences
     misc_feature    652..686
                     /label=New Feature
     misc_feature    652..668
                     /label=transferred sequence from attB1
     misc_feature    680..684
                     /label=TOPO recognition site 1
     misc_feature    680..683
                     /label=Topo recognition site 1
     misc_feature    685..686
                     /label=overhang
     misc_feature    689..6847
                     /label=Trip12
     misc_feature    689..6847
                     /label=Trip12
                     /label=Trip12(1)
     misc_feature    6856..6866
                     /label=Trabsferred sequences
                     /label=Trabsferred sequences(1)
     misc_feature    6856..6865
                     /label=New Feature
                     /label=New Feature(1)
     misc_feature    6857..6956
                     /label=attL2
     misc_feature    6857..6866
                     /label=transferred sequence from attB2
     misc_feature    7126..7935
                     /label=Kan
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccat ggaacaaaaa ctcatctcag aagaggatct
      721 ggcagcatcc aaccggccta ataacaatcc aggggggtca ctgcgacgtt cacagaggaa
      781 cactgccggg gcccaaccac aagacgactc aataggagga aggtcacatt tagggcaggc
      841 aaaacataag ggatatagcc ctcctgagag tagaaaatct aattctaagg cacccaaagt
      901 gcagtctaat actacttctg aactgtcaag gggacacctt tctaaaagaa gctgcagttc
      961 atcatctgct gtgatagttc cacaaccaga ggatccagac agagccaata cttcagaaag
     1021 acaaaaaacg gggcaggtgc ctaagaaaga caattctcga ggagtgaagc gcagtgctag
     1081 tccagactac aacaggacca attctcctag ctctgcaaaa aaaccaaaag cacttcagca
     1141 tactgaatct ccctcagaaa caaataagcc acatagtaag tcaaagaaga gacatttaga
     1201 ccaggagcaa caactgaaat ctgcacaatc accatcaaca agcaaggctc ataccaggaa
     1261 gagtggggcc actggcggtt cacggagtca gaaaagaaaa aggacagaga gttcttgtgt
     1321 aaagagtggc tccgggtctg aatcaactgg tgcagaagag agatctgcga aacctaccaa
     1381 gctggcttca aaatcagcca cctcagccaa agctgggtgt agcaccatca ctgattcttc
     1441 ttctgctgcc tctacttcct cctcgtcttc tgctgtagcc tcggcctcct ccactgtacc
     1501 accaggtgcc agagtgaaac aaggaaaaga tcagaacaag gccaggcgtt cccgttcagc
     1561 gtccagtccc agccccagaa gaagtagcag ggaaaaggaa cagagtaaaa ctggtggctc
     1621 ttcaaaattt gattgggctg ctcgtttcag ccctaaagtt agccttccta aaacaaaact
     1681 gtctcttcca gggtcttcta agtcagagac atcaaaacct ggaccttctg gattacaggc
     1741 caaattagca agtttaagaa aatctacgaa gaaacgcagt gagtctccac ctgctgagct
     1801 ccccagtttg aggcggagca cacgccaaaa gaccacgggc tcctgtgcta gtaccagtcg
     1861 gcgaggctct ggcctgggca aaagaggagc agctgaagct cgtcgacagg agaaaatggc
     1921 agaccctgaa agcaaccagg aggcagtaaa ttcttcagct gctcggacag atgaagctcc
     1981 ccaaggagct gcagcctcta gttctgttgc aggggctgtt ggcatgacca cctctgggga
     2041 gagtgaatca gatgattccg agatgggacg tttgcaagct ttgttagagg caaggggtct
     2101 tccccctcac ctatttggtc ctcttggtcc tcggatgtca cagcttttcc atagaacaat
     2161 tggaagtgga gctagttcta aggcccagca gctactacaa ggattgcaag ccagtgatga
     2221 aagtcaacag cttcaggcag ttattgagat gtgtcagtta ctggtcatgg gaaatgagga
     2281 gacactggga gggtttcctg tcaagagtgt tgttccagct ttgattacgt tacttcagat
     2341 ggagcacaat tttgatatta tgaaccatgc ttgtcgagcc ttaacataca tgatggaagc
     2401 acttcctcga tcttctgctg ttgtagtaga tgctattcct gtctttttag aaaagctgca
     2461 agttattcag tgtattgatg tggcagagca ggccttgact gccttggaga tgttgtcacg
     2521 gagacatagt aaagccattc tacaggcggg tggtttggca gactgcttgc tgtacctaga
     2581 attcttcagc ataaatgccc aaagaaatgc attagcaatt gcagctaatt gctgccagag
     2641 tatcacgcca gatgaatttc attttgtggc agattcactc ccattgctaa cccaaaggct
     2701 aacacatcag gataaaaagt cagtagaaag cacttgcctt tgttttgcac gcctagtgga
     2761 caacttccag catgaggaga atttactcca gcaggttgct tccaaagatc tgcttacaaa
     2821 tgttcaacag ctgttggtag tgactccacc cattttaagt tctgggatgt ttataatggt
     2881 ggttcgcatg ttttctctga tgtgttccaa ctgtccaact ttagctgttc aacttatgaa
     2941 acaaaacatt gcagaaacgc ttcactttct cctgtgtggt gcctccaatg gaagttgtca
     3001 ggaacagatt gatcttgttc cacgaagccc tcaagagttg tatgaactga catctctgat
     3061 ttgtgaactt atgccatgtt taccaaaaga aggcattttt gcagttgata ccatgttgaa
     3121 gaagggaaat gcacagaaca cagatggtgc gatatggcag tggcgtgatg atcggggcct
     3181 ctggcatcca tataacagga ttgacagccg gatcattgag caaatcaatg aggacacggg
     3241 aacagcacgt gccattcaga gaaaacctaa cccgttagcc aatagtaaca ctagtggata
     3301 ttcagagtca aagaaggatg atgctcgagc acagcttatg aaagaggatc cggaactggc
     3361 taagtctttt attaagacat tatttggtgt tctttatgaa gtgtatagtt cctcagcagg
     3421 acctgcggtc agacataagt gccttagagc aattcttagg ataatttatt ttgcggatgc
     3481 tgaacttctg aaggatgttc tgaaaaatca tgctgtttca agtcacattg cttccatgct
     3541 gtcaagccaa gacctgaaga tagtagtggg agcacttcag atggcagaaa ttttaatgca
     3601 gaagttacct gatattttta gtgtttactt cagaagagaa ggtgtaatgc atcaagtaaa
     3661 acacttagca gaatcagagt ctttgttgac aagtccacca aaggcatgta cgaatggatc
     3721 gggatcaatg ggatccacaa cttcagtcag cagtgggaca gccacagctg ccactcatgc
     3781 tgcagctgac ttgggatcac ccagcttgca gcacagcagg gatgattctt tagatctcag
     3841 ccctcaaggt cgattaagtg atgttctaaa gagaaaacga ctgccaaaac gagggccaag
     3901 aaggccaaag tactcacctc caagagatga tgacaaagta gacaatcaag ctaaaagccc
     3961 caccactact cagtcaccta aatcttcttt cctggcaagc ttgaatccaa aaacatgggg
     4021 aaggttaagt acacagtcca acagcaacaa cattgagcca gcacggactg cgggaggtag
     4081 tggccttgcc agggctgcct caaaggatac catctccaat aatagagaaa aaattaaagg
     4141 ttggattaag gagcaggcac ataaatttgt agaacgttat ttcagttctg agaatatgga
     4201 tggaagcaac cctgcattga atgtccttca gagactttgt gctgcaaccg aacaactcaa
     4261 cctccaggtg gatggtggag ctgagtgcct tgtagaaatc cgtagcatag tctcagagtc
     4321 agatgtttca tcatttgaaa tccaacatag tggatttgtg aagcagctgt tgctttattt
     4381 gacatctaaa agtgaaaagg atgctgtgag cagagagatc agattaaagc gatttcttca
     4441 tgtatttttt tcttctccac ttcctggaga agagcccatt ggaagagtgg aaccagtggg
     4501 taatgcacct ttgttggcat tagttcacaa gatgaacaac tgcctcagcc agatggaaca
     4561 atttccagtc aaagtacatg atttccctag tggaaatggg acaggaggca gcttttctct
     4621 caacagagga tcacaggctt taaaattttt caacacacat caattaaaat gccagttaca
     4681 aaggcatcca gactgtgcaa atgtgaagca gtggaagggt ggacctgtca agattgaccc
     4741 tctggctttg gtacaagcca tcgagagata ccttgtagtt agagggtatg gaagagtaag
     4801 agaagatgat gaagacagcg atgacgatgg atcagatgag gaaatagatg agtctctggc
     4861 tgctcagttc ctaaattcag gaaatgtaag acacaggctg cagttttata ttggagaaca
     4921 tttgctgccg tataacatga ctgtgtatca ggcagtacgg cagtttagta tacaggctga
     4981 agatgaaaga gaatccacag atgatgagag caatcctcta ggcagagctg gtatttggac
     5041 aaagactcat acaatatggt ataaacctgt gagagaggat gaagaaagta ataaagattg
     5101 tgttggtggt aaaagaggaa gagcccaaac agctccaacg aaaacttccc ctagaaatgc
     5161 aaaaaagcat gatgagttat ggcacgatgg agtgtgccca tcagtatcaa atcctttaga
     5221 agtttacctc attcccacac cacctgaaaa tataacattt gaagacccgt cattagatgt
     5281 gatccttctt ttaagagttt tacatgctat cagtcgatac tggtattact tgtatgataa
     5341 tgcaatgtgc aaggaaatta ttccaactag tgaatttatt aacagtaagt taacagcaaa
     5401 agcaaatagg caacttcaag atcctttagt aatcatgaca ggaaacatcc caacatggct
     5461 tactgagcta ggaaaaacct gcccattttt ctttcctttt gatacccggc aaatgctttt
     5521 ttatgtaact gcatttgatc gggaccgagc aatgcaaaga ttacttgata ccaacccaga
     5581 aatcaaccag tctgattctc aagatagcag agttgcacct agattggata gaaaaaaacg
     5641 tactgtgaac cgagaggagc tgctgaaaca ggcggagtct gtgatgcagg acctcggcag
     5701 ctcacgggcc atgttagaaa tccagtatga aaatgaggtt ggtacaggtc ttgggcctac
     5761 actggagttt tatgcgcttg tatctcagga actacagaga gctgacttgg gtctttggag
     5821 aggtgaagaa gtaactctta gcaatccaaa agggagccaa gaagggacca agtatattca
     5881 aaacctccag ggcctgtttg cgcttccctt tggtaggaca gcaaagccag ctcatatcgc
     5941 aaaggttaag atgaagtttc gcttcttagg aaaattaatg gccaaggcta tcatggattt
     6001 cagattggtg gaccttcccc ttggcttacc cttttataaa tggatgctac ggcaagaaac
     6061 ttcactgaca tcacacgatt tgtttgacat cgacccagtt gtagccagat cagtttatca
     6121 cctagaagac attgtcagac agaagaaaag acttgaacaa gataaatccc agaccaaaga
     6181 gagtctacag tatgcattag aaaccttgac tatgaatggc tgctcagttg aagatctagg
     6241 actggatttc actctgccag ggtttcccaa tatcgaactg aagaaaggag ggaaggatat
     6301 accagtcact atccacaatt tagaggagta tctaagactg gttatattct gggcactaaa
     6361 tgaaggcgtt tctaggcaat ttgattcgtt cagagatgga tttgaatcag tcttcccact
     6421 cagtcatctt cagtacttct acccggagga actggatcag ctcctttgtg gcagtaaagc
     6481 agacacttgg gatgcaaaga cactgatgga atgctgtagg cctgatcatg gttatactca
     6541 tgacagtcgg gctgtgaagt ttttgtttga gattctcagt agttttgata atgagcagca
     6601 gaggttattt ctccagtttg tgactggtag cccaagattg cctgttggag gattccggag
     6661 tttgaatcca cctttgacaa ttgtccgaaa gacgtttgaa tcaacagaaa acccagatga
     6721 cttcttgccc tctgtaatga cttgtgtgaa ctatcttaag ttgccggact attcaagcat
     6781 tgagataatg cgtgaaaaac tgttgatagc agcaagagaa gggcagcagt cgttccatct
     6841 ttcctgaggc gcgccgaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     6901 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     6961 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     7021 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     7081 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     7141 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     7201 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     7261 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     7321 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     7381 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     7441 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     7501 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     7561 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     7621 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     7681 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     7741 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     7801 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     7861 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     7921 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     7981 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     8041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     8101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8221 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8461 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8701 ctggcctttt gctggccttt tgctcacatg tt
//
