LOCUS       1301.pLVX-cmv-My        9458 bp    DNA     linear   UNA 03-MAR-2016
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 13, 2025 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        2201..2727
                     /label=CMV promoter
     misc_feature    2816..2864
                     /label=AttA
     misc_feature    2869..2907
                     /label=Myc-tag seq
     misc_feature    2869..2872
                     /label=NcoI site
     misc_feature    2908..4080
                     /label=HECT domain
     misc_feature    4081..4088
                     /label=AscI site
     misc_feature    4089..4133
                     /label=AttB
     misc_feature    4159..4766
                     /label=IRES
     misc_feature    4767..5366
                     /label=Puro
     primer_bind     complement(6932..6952)
                     /label=M13-rev
     misc_binding    complement(6958..6980)
                     /label=LacO
     rep_origin      complement(7320..8002)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(8100..8759)
                     /label=AmpR
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactaga
     2821 tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat ggaacaaaaa
     2881 ctcatctcag aagaggatct ggcagcagag tctgtgatgc aggacctcgg cagctcacgg
     2941 gccatgttag aaatccagta tgaaaatgag gttggtacag gtcttgggcc tacactggag
     3001 ttttatgcgc ttgtatctca ggaactacag agagctgact tgggtctttg gagaggtgaa
     3061 gaagtaactc ttagcaatcc aaaagggagc caagaaggga ccaagtatat tcaaaacctc
     3121 cagggcctgt ttgcgcttcc ctttggtagg acagcaaagc cagctcatat cgcaaaggtt
     3181 aagatgaagt ttcgcttctt aggaaaatta atggccaagg ctatcatgga tttcagattg
     3241 gtggaccttc cccttggctt acccttttat aaatggatgc tacggcaaga aacttcactg
     3301 acatcacacg atttgtttga catcgaccca gttgtagcca gatcagttta tcacctagaa
     3361 gacattgtca gacagaagaa aagacttgaa caagataaat cccagaccaa agagagtcta
     3421 cagtatgcat tagaaacctt gactatgaat ggctgctcag ttgaagatct aggactggat
     3481 ttcactctgc cagggtttcc caatatcgaa ctgaagaaag gagggaagga tataccagtc
     3541 actatccaca atttagagga gtatctaaga ctggttatat tctgggcact aaatgaaggc
     3601 gtttctaggc aatttgattc gttcagagat ggatttgaat cagtcttccc actcagtcat
     3661 cttcagtact tctacccgga ggaactggat cagctccttt gtggcagtaa agcagacact
     3721 tgggatgcaa agacactgat ggaatgctgt aggcctgatc atggttatac tcatgacagt
     3781 cgggctgtga agtttttgtt tgagattctc agtagttttg ataatgagca gcagaggtta
     3841 tttctccagt ttgtgactgg tagcccaaga ttgcctgttg gaggattccg gagtttgaat
     3901 ccacctttga caattgtccg aaagacgttt gaatcaacag aaaacccaga tgacttcttg
     3961 ccctctgtaa tgacttgtgt gaactatctt aagttgccgg actattcaag cattgagata
     4021 atgcgtgaaa aactgttgat agcagcaaga gaagggcagc agtcgttcca tctttcctga
     4081 ggcgcgccaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt gatctagttc
     4141 tagagcggcc gcggatcccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     4201 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     4261 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     4321 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     4381 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     4441 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     4501 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     4561 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     4621 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     4681 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     4741 cacaacccac aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca
     4801 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     4861 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     4921 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     4981 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     5041 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     5101 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     5161 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     5221 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     5281 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     5341 gcatgacccg caagcccggt gcctagacgc gtctggaaca atcaacctct ggattacaaa
     5401 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5461 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5521 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5581 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5641 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5701 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5761 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     5821 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5881 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5941 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     6001 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     6061 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     6121 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagagg ggactggaag
     6181 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     6241 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     6301 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     6361 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     6421 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     6481 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     6541 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     6601 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6661 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6721 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6781 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     6841 ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacattg ctagcgttta
     6901 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6961 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7021 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7081 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7141 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7201 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7261 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7321 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7381 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7441 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7501 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7561 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7621 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7681 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7741 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7801 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7861 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7921 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7981 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8041 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     8101 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8161 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     8221 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     8281 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8341 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8401 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8461 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8521 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8581 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8641 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8701 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8761 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8821 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8881 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8941 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9001 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9061 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
     9121 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     9181 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     9241 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     9301 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9361 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga
     9421 aattgtattt gttaaatatg tactacaaac ttagtagt
//
