LOCUS       1304.pLVX-cmv-HE        9837 bp    DNA     circular SYN 03-MAR-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 13, 2025 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2201..2727
                     /label=CMV promoter
     misc_feature    2816..2864
                     /label=AttA
     misc_feature    2869..2907
                     /label=Myc-tag seq
     misc_feature    2869..2872
                     /label=NcoI site
     misc_feature    2908..4080
                     /label=HECT domain
     misc_feature    4081..4088
                     /label=AscI site
     misc_feature    4089..4133
                     /label=AttB
     misc_feature    4758..5792
                     /label=Hyg
     primer_bind     complement(7358..7378)
                     /label=M13-rev
     misc_binding    complement(7384..7406)
                     /label=LacO
     rep_origin      complement(7746..8428)
                     /direction=LEFT
                     /label=ColE1 origin
     CDS             complement(8526..9185)
                     /label=AmpR
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactaga
     2821 tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat ggaacaaaaa
     2881 ctcatctcag aagaggatct ggcagcagag tctgtgatgc aggacctcgg cagctcacgg
     2941 gccatgttag aaatccagta tgaaaatgag gttggtacag gtcttgggcc tacactggag
     3001 ttttatgcgc ttgtatctca ggaactacag agagctgact tgggtctttg gagaggtgaa
     3061 gaagtaactc ttagcaatcc aaaagggagc caagaaggga ccaagtatat tcaaaacctc
     3121 cagggcctgt ttgcgcttcc ctttggtagg acagcaaagc cagctcatat cgcaaaggtt
     3181 aagatgaagt ttcgcttctt aggaaaatta atggccaagg ctatcatgga tttcagattg
     3241 gtggaccttc cccttggctt acccttttat aaatggatgc tacggcaaga aacttcactg
     3301 acatcacacg atttgtttga catcgaccca gttgtagcca gatcagttta tcacctagaa
     3361 gacattgtca gacagaagaa aagacttgaa caagataaat cccagaccaa agagagtcta
     3421 cagtatgcat tagaaacctt gactatgaat ggctgctcag ttgaagatct aggactggat
     3481 ttcactctgc cagggtttcc caatatcgaa ctgaagaaag gagggaagga tataccagtc
     3541 actatccaca atttagagga gtatctaaga ctggttatat tctgggcact aaatgaaggc
     3601 gtttctaggc aatttgattc gttcagagat ggatttgaat cagtcttccc actcagtcat
     3661 cttcagtact tctacccgga ggaactggat cagctccttt gtggcagtaa agcagacact
     3721 tgggatgcaa agacactgat ggaatgctgt aggcctgatc atggttatac tcatgacagt
     3781 cgggctgtga agtttttgtt tgagattctc agtagttttg ataatgagca gcagaggtta
     3841 tttctccagt ttgtgactgg tagcccaaga ttgcctgttg gaggattccg gagtttgaat
     3901 ccacctttga caattgtccg aaagacgttt gaatcaacag aaaacccaga tgacttcttg
     3961 ccctctgtaa tgacttgtgt gaactatctt aagttgccgg actattcaag cattgagata
     4021 atgcgtgaaa aactgttgat agcagcaaga gaagggcagc agtcgttcca tctttcctga
     4081 ggcgcgccaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt gatctagttc
     4141 tagagcggcc gcggatcccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     4201 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     4261 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     4321 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     4381 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     4441 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     4501 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     4561 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     4621 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     4681 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     4741 cacaacccgt accaaagatg gatagatccg gaaagcctga actcaccgcg acgtctgtcg
     4801 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     4861 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     4921 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     4981 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     5041 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     5101 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     5161 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     5221 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     5281 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     5341 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     5401 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     5461 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     5521 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     5581 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     5641 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     5701 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     5761 gccccagcac tcgtccgagg gcaaaggaat agacgcgtct ggaacaatca acctctggat
     5821 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5881 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5941 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6001 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6061 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     6121 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6181 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     6241 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6301 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6361 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     6421 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     6481 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     6541 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     6601 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     6661 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     6721 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     6781 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     6841 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     6901 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     6961 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     7021 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     7081 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7141 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7201 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7261 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     7321 cgtttaccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     7381 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     7441 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     7501 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     7561 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     7621 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     7681 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     7741 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     7801 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     7861 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     7921 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     7981 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     8041 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     8101 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     8161 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     8221 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     8281 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     8341 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     8401 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     8461 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     8521 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     8581 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     8641 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     8701 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     8761 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     8821 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     8881 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     8941 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     9001 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     9061 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     9121 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     9181 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     9241 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     9301 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     9361 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     9421 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     9481 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcaactt
     9541 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     9601 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     9661 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     9721 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     9781 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
